Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection
The aim of this study is to establish an ion chromatography method for simultaneous determination of glucose, fructose, arabinose, galactose, mannose and ribose in beef hydrolysates. The pretreatment and chromatography conditions were optimized. Finally, the protein was precipitated by 1% trichloroa...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070122 |
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| author | Xiuhong CHEN Peng JI Guoliang HE Ran XIA Zuming LI Jia LIU |
| author_facet | Xiuhong CHEN Peng JI Guoliang HE Ran XIA Zuming LI Jia LIU |
| author_sort | Xiuhong CHEN |
| collection | DOAJ |
| description | The aim of this study is to establish an ion chromatography method for simultaneous determination of glucose, fructose, arabinose, galactose, mannose and ribose in beef hydrolysates. The pretreatment and chromatography conditions were optimized. Finally, the protein was precipitated by 1% trichloroacetic acid and polysaccharide was hydrolyzed by 4 mol/L sulfuric acid. The CarboPacTM PA20 column was selected for separation. 5 mmol/L sodium hydroxide solution was used as the eluent for gradient elution, with the detection of pulse amperometric detector(PAD). The results showed that the six sugars could be separated completely under the above conditions, the linear relationship of glucose and other sugars within the range of 0.25~2.50 μg/mL and 0.025~0.25 μg/mL, respectively, was good. And the correlation coefficient was more than 0.998. The RSD of method was within 2.3%~5.5%, and the recovery was within 79.6%~116.5%. The method has the advantages of simple operation, high sensitivity and good resolution, and can be used to determine the content of sugars in beef hydrolysates accurately and rapidly. |
| first_indexed | 2024-04-11T06:49:19Z |
| format | Article |
| id | doaj.art-88937af8d6804392aed85f4707c4b49f |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:49:19Z |
| publishDate | 2022-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-88937af8d6804392aed85f4707c4b49f2022-12-22T04:39:15ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-06-01431126727510.13386/j.issn1002-0306.20210701222021070122-11Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric DetectionXiuhong CHEN0Peng JI1Guoliang HE2Ran XIA3Zuming LI4Jia LIU5Internal Trade Food Science Research Institute Co., Ltd., National Non-staple Food Quality Supervision and Inspection Center, Beijing 102209, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaInternal Trade Food Science Research Institute Co., Ltd., National Non-staple Food Quality Supervision and Inspection Center, Beijing 102209, ChinaInternal Trade Food Science Research Institute Co., Ltd., National Non-staple Food Quality Supervision and Inspection Center, Beijing 102209, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaInternal Trade Food Science Research Institute Co., Ltd., National Non-staple Food Quality Supervision and Inspection Center, Beijing 102209, ChinaThe aim of this study is to establish an ion chromatography method for simultaneous determination of glucose, fructose, arabinose, galactose, mannose and ribose in beef hydrolysates. The pretreatment and chromatography conditions were optimized. Finally, the protein was precipitated by 1% trichloroacetic acid and polysaccharide was hydrolyzed by 4 mol/L sulfuric acid. The CarboPacTM PA20 column was selected for separation. 5 mmol/L sodium hydroxide solution was used as the eluent for gradient elution, with the detection of pulse amperometric detector(PAD). The results showed that the six sugars could be separated completely under the above conditions, the linear relationship of glucose and other sugars within the range of 0.25~2.50 μg/mL and 0.025~0.25 μg/mL, respectively, was good. And the correlation coefficient was more than 0.998. The RSD of method was within 2.3%~5.5%, and the recovery was within 79.6%~116.5%. The method has the advantages of simple operation, high sensitivity and good resolution, and can be used to determine the content of sugars in beef hydrolysates accurately and rapidly.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070122ion chromatographypulsed amperometric detectionbeefhydrolysatesmonosaccharides |
| spellingShingle | Xiuhong CHEN Peng JI Guoliang HE Ran XIA Zuming LI Jia LIU Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection Shipin gongye ke-ji ion chromatography pulsed amperometric detection beef hydrolysates monosaccharides |
| title | Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection |
| title_full | Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection |
| title_fullStr | Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection |
| title_full_unstemmed | Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection |
| title_short | Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection |
| title_sort | simultaneous determination of six sugars in beef hydrolysates by ion chromatography with pulsed amperometric detection |
| topic | ion chromatography pulsed amperometric detection beef hydrolysates monosaccharides |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070122 |
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