Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail.
Different reproductive strategies and the transition to asexuality can be associated with microbial symbionts. Whether such a link exists within mollusks has never been evaluated. We took the first steps towards addressing this possibility by performing pyrosequencing of bacterial 16S rRNA genes ass...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5001651?pdf=render |
| _version_ | 1811309869437812736 |
|---|---|
| author | Cristina Takacs-Vesbach Kayla King David Van Horn Katelyn Larkin Maurine Neiman |
| author_facet | Cristina Takacs-Vesbach Kayla King David Van Horn Katelyn Larkin Maurine Neiman |
| author_sort | Cristina Takacs-Vesbach |
| collection | DOAJ |
| description | Different reproductive strategies and the transition to asexuality can be associated with microbial symbionts. Whether such a link exists within mollusks has never been evaluated. We took the first steps towards addressing this possibility by performing pyrosequencing of bacterial 16S rRNA genes associated with Potamopyrgus antipodarum, a New Zealand freshwater snail. A diverse set of 60 tissue collections from P. antipodarum that were genetically and geographically distinct and either obligately sexual or asexual were included, which allowed us to evaluate whether reproductive mode was associated with a particular bacterial community. 2624 unique operational taxonomic units (OTU, 97% DNA similarity) were detected, which were distributed across ~30 phyla. While alpha diversity metrics varied little among individual samples, significant differences in bacterial community composition and structure were detected between sexual and asexual snails, as well as among snails from different lakes and genetic backgrounds. The mean dissimilarity of the bacterial communities between the sexual and asexual P. antipodarum was 90%, largely driven by the presence of Rickettsiales in sexual snails and Rhodobacter in asexual snails. Our study suggests that there might be a link between reproductive mode and the bacterial microbiome of P. antipodarum, though a causal connection requires additional study. |
| first_indexed | 2024-04-13T09:48:38Z |
| format | Article |
| id | doaj.art-889aa5ded4514b0fa3534c473656e92d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T09:48:38Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-889aa5ded4514b0fa3534c473656e92d2022-12-22T02:51:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016105010.1371/journal.pone.0161050Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail.Cristina Takacs-VesbachKayla KingDavid Van HornKatelyn LarkinMaurine NeimanDifferent reproductive strategies and the transition to asexuality can be associated with microbial symbionts. Whether such a link exists within mollusks has never been evaluated. We took the first steps towards addressing this possibility by performing pyrosequencing of bacterial 16S rRNA genes associated with Potamopyrgus antipodarum, a New Zealand freshwater snail. A diverse set of 60 tissue collections from P. antipodarum that were genetically and geographically distinct and either obligately sexual or asexual were included, which allowed us to evaluate whether reproductive mode was associated with a particular bacterial community. 2624 unique operational taxonomic units (OTU, 97% DNA similarity) were detected, which were distributed across ~30 phyla. While alpha diversity metrics varied little among individual samples, significant differences in bacterial community composition and structure were detected between sexual and asexual snails, as well as among snails from different lakes and genetic backgrounds. The mean dissimilarity of the bacterial communities between the sexual and asexual P. antipodarum was 90%, largely driven by the presence of Rickettsiales in sexual snails and Rhodobacter in asexual snails. Our study suggests that there might be a link between reproductive mode and the bacterial microbiome of P. antipodarum, though a causal connection requires additional study.http://europepmc.org/articles/PMC5001651?pdf=render |
| spellingShingle | Cristina Takacs-Vesbach Kayla King David Van Horn Katelyn Larkin Maurine Neiman Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. PLoS ONE |
| title | Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. |
| title_full | Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. |
| title_fullStr | Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. |
| title_full_unstemmed | Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. |
| title_short | Distinct Bacterial Microbiomes in Sexual and Asexual Potamopyrgus antipodarum, a New Zealand Freshwater Snail. |
| title_sort | distinct bacterial microbiomes in sexual and asexual potamopyrgus antipodarum a new zealand freshwater snail |
| url | http://europepmc.org/articles/PMC5001651?pdf=render |
| work_keys_str_mv | AT cristinatakacsvesbach distinctbacterialmicrobiomesinsexualandasexualpotamopyrgusantipodarumanewzealandfreshwatersnail AT kaylaking distinctbacterialmicrobiomesinsexualandasexualpotamopyrgusantipodarumanewzealandfreshwatersnail AT davidvanhorn distinctbacterialmicrobiomesinsexualandasexualpotamopyrgusantipodarumanewzealandfreshwatersnail AT katelynlarkin distinctbacterialmicrobiomesinsexualandasexualpotamopyrgusantipodarumanewzealandfreshwatersnail AT maurineneiman distinctbacterialmicrobiomesinsexualandasexualpotamopyrgusantipodarumanewzealandfreshwatersnail |