Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles
A notable diagnostic for the detection of hemolytic diseases is bilirubin, a by-product of haemoglobin breakdown. The concentration of bilirubin ranges from 0.3 to 1.9 mg in 100 mL of blood. Low blood bilirubin levels are associated with a greater risk of coronary heart disease and anaemia. Hyperbil...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/11/1845 |
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| author | Nigar Anzar Shariq Suleman Rocky Kumar Rachna Rawal Chandra Shekhar Pundir Roberto Pilloton Jagriti Narang |
| author_facet | Nigar Anzar Shariq Suleman Rocky Kumar Rachna Rawal Chandra Shekhar Pundir Roberto Pilloton Jagriti Narang |
| author_sort | Nigar Anzar |
| collection | DOAJ |
| description | A notable diagnostic for the detection of hemolytic diseases is bilirubin, a by-product of haemoglobin breakdown. The concentration of bilirubin ranges from 0.3 to 1.9 mg in 100 mL of blood. Low blood bilirubin levels are associated with a greater risk of coronary heart disease and anaemia. Hyperbilirubinemia results from a serum bilirubin level of more than 2.5 mg/100 mL. Therefore, it is very crucial to check the serum bilirubin level. Analytical equipment for point-of-care testing must be portable, small, and affordable. A unique method is used to detect bilirubin selectively using paper-based screen-printed carbon electrodes that were covalently linked with nanoparticles, that serves as a key biomarker for jaundice. In order to create an electrochemical biosensor, bilirubin oxidase was immobilised on electrodes modified with AgNPs. The morphology of Ag nanoparticles in terms of size and shape was determined using both UV- Vis Spectroscopy and transmission electron microscopy (TEM). The biosensor’s analytical response was assessed using potentiostat (Cyclic voltammetry (CV) and linear sweep voltammetry (LSV)). The developed paper-based sensor provided optimum feedback and a broad linear range of 1 to 9 µg/mL for bilirubin, with a lower LOD of 1 µg/mL. Through tests of bilirubin in artificial blood serum, the viability is confirmed. The method that is being used makes it possible to create and use an inexpensive, miniature electrochemical sensor. |
| first_indexed | 2024-03-09T18:50:03Z |
| format | Article |
| id | doaj.art-88a128c7303c4ce18e23a7bf41868755 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T18:50:03Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-88a128c7303c4ce18e23a7bf418687552023-11-24T05:54:06ZengMDPI AGMicromachines2072-666X2022-10-011311184510.3390/mi13111845Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver NanoparticlesNigar Anzar0Shariq Suleman1Rocky Kumar2Rachna Rawal3Chandra Shekhar Pundir4Roberto Pilloton5Jagriti Narang6Department of Biotechnology, Jamia Hamdard University, New Delhi 110062, IndiaDepartment of Biotechnology, Jamia Hamdard University, New Delhi 110062, IndiaDepartment of Biotechnology, Jamia Hamdard University, New Delhi 110062, IndiaDepartment of Physics and Astrophysics, University of Delhi, Delhi 110007, IndiaDepartment of Biochemistry, Maharshi Dayanand University, Rohtak 124001, IndiaInstitute of Crystallography of National Research council (IC-CNR), Monterotondo, I-00015 Rome, ItalyDepartment of Biotechnology, Jamia Hamdard University, New Delhi 110062, IndiaA notable diagnostic for the detection of hemolytic diseases is bilirubin, a by-product of haemoglobin breakdown. The concentration of bilirubin ranges from 0.3 to 1.9 mg in 100 mL of blood. Low blood bilirubin levels are associated with a greater risk of coronary heart disease and anaemia. Hyperbilirubinemia results from a serum bilirubin level of more than 2.5 mg/100 mL. Therefore, it is very crucial to check the serum bilirubin level. Analytical equipment for point-of-care testing must be portable, small, and affordable. A unique method is used to detect bilirubin selectively using paper-based screen-printed carbon electrodes that were covalently linked with nanoparticles, that serves as a key biomarker for jaundice. In order to create an electrochemical biosensor, bilirubin oxidase was immobilised on electrodes modified with AgNPs. The morphology of Ag nanoparticles in terms of size and shape was determined using both UV- Vis Spectroscopy and transmission electron microscopy (TEM). The biosensor’s analytical response was assessed using potentiostat (Cyclic voltammetry (CV) and linear sweep voltammetry (LSV)). The developed paper-based sensor provided optimum feedback and a broad linear range of 1 to 9 µg/mL for bilirubin, with a lower LOD of 1 µg/mL. Through tests of bilirubin in artificial blood serum, the viability is confirmed. The method that is being used makes it possible to create and use an inexpensive, miniature electrochemical sensor.https://www.mdpi.com/2072-666X/13/11/1845bilirubinelectrochemicalserumnanoparticlesjaundice |
| spellingShingle | Nigar Anzar Shariq Suleman Rocky Kumar Rachna Rawal Chandra Shekhar Pundir Roberto Pilloton Jagriti Narang Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles Micromachines bilirubin electrochemical serum nanoparticles jaundice |
| title | Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles |
| title_full | Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles |
| title_fullStr | Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles |
| title_full_unstemmed | Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles |
| title_short | Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles |
| title_sort | electrochemical sensor for bilirubin detection using paper based screen printed electrodes functionalized with silver nanoparticles |
| topic | bilirubin electrochemical serum nanoparticles jaundice |
| url | https://www.mdpi.com/2072-666X/13/11/1845 |
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