Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat
The present study aimed to develop an isotope protocol to achieve equilibrium of 13CO2 in breath of cats during carbon oxidation studies using L-[1-13C]-Phenylalanine (L-[1-13C]-Phe), provided orally in repeated meals. One adult male cat was used in two experiments. In each experiment, three isotope...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679023000447/type/journal_article |
| _version_ | 1797817361696292864 |
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| author | Julia Guazzelli Pezzali Jocelyn G. Lambie Stuart M. Phillips Anna K. Shoveller |
| author_facet | Julia Guazzelli Pezzali Jocelyn G. Lambie Stuart M. Phillips Anna K. Shoveller |
| author_sort | Julia Guazzelli Pezzali |
| collection | DOAJ |
| description | The present study aimed to develop an isotope protocol to achieve equilibrium of 13CO2 in breath of cats during carbon oxidation studies using L-[1-13C]-Phenylalanine (L-[1-13C]-Phe), provided orally in repeated meals. One adult male cat was used in two experiments. In each experiment, three isotope protocols were tested in triplicate using the same cat. During carbon oxidation study days, the cat was offered thirteen small meals to achieve and maintain a physiological fed state. In experiment 1, the isotope protocols tested (A, B and C) had a similar priming dose of NaH13CO3 (0⋅176 mg/kg; offered in meal 6), but different priming [4⋅8 mg/kg (A) or 9⋅4 mg/kg (B and C); provided in meal 6] and constant [1⋅04 mg/kg (A and B) or 2⋅4 mg/kg (C); offered in meals 6–13] doses of L-[1-13C]-Phe. In experiment 2, the isotope protocols tested (D, E and F) had similar priming (4⋅8 mg/kg; provided in meal 5) and constant (1⋅04 mg/kg; provided in meals 5–13) doses of L-[1-13C]-Phe, but increasing priming doses of NaH13CO3 (D: 0⋅264, E: 0⋅352, F: 0⋅44 mg/kg; provided in meal 4). Breath samples were collected using respiration chambers (25-min intervals) and CO2 trapping to determine 13CO2:12CO2. Isotopic steady state was defined as the enrichment of 13CO2, above background samples, remaining constant in at least the last three samples. Treatment F resulted in the earliest achievement of 13CO2 steady state in the cat's breath. This feeding and isotope protocol can be used in future studies aiming to study amino acid metabolism in cats. |
| first_indexed | 2024-03-13T08:52:21Z |
| format | Article |
| id | doaj.art-88a1b011059f49f19516dd7e88799228 |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-03-13T08:52:21Z |
| publishDate | 2023-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-88a1b011059f49f19516dd7e887992282023-05-29T09:04:50ZengCambridge University PressJournal of Nutritional Science2048-67902023-01-011210.1017/jns.2023.44Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic catJulia Guazzelli Pezzali0Jocelyn G. Lambie1Stuart M. Phillips2Anna K. Shoveller3https://orcid.org/0000-0002-2584-4774Department of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, ON, Canada Department of Animal Science, Iowa State University, Ames, IA, United StatesDepartment of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, ON, CanadaDepartment of Kinesiology, McMaster University, Hamilton, ON, CanadaDepartment of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, ON, CanadaThe present study aimed to develop an isotope protocol to achieve equilibrium of 13CO2 in breath of cats during carbon oxidation studies using L-[1-13C]-Phenylalanine (L-[1-13C]-Phe), provided orally in repeated meals. One adult male cat was used in two experiments. In each experiment, three isotope protocols were tested in triplicate using the same cat. During carbon oxidation study days, the cat was offered thirteen small meals to achieve and maintain a physiological fed state. In experiment 1, the isotope protocols tested (A, B and C) had a similar priming dose of NaH13CO3 (0⋅176 mg/kg; offered in meal 6), but different priming [4⋅8 mg/kg (A) or 9⋅4 mg/kg (B and C); provided in meal 6] and constant [1⋅04 mg/kg (A and B) or 2⋅4 mg/kg (C); offered in meals 6–13] doses of L-[1-13C]-Phe. In experiment 2, the isotope protocols tested (D, E and F) had similar priming (4⋅8 mg/kg; provided in meal 5) and constant (1⋅04 mg/kg; provided in meals 5–13) doses of L-[1-13C]-Phe, but increasing priming doses of NaH13CO3 (D: 0⋅264, E: 0⋅352, F: 0⋅44 mg/kg; provided in meal 4). Breath samples were collected using respiration chambers (25-min intervals) and CO2 trapping to determine 13CO2:12CO2. Isotopic steady state was defined as the enrichment of 13CO2, above background samples, remaining constant in at least the last three samples. Treatment F resulted in the earliest achievement of 13CO2 steady state in the cat's breath. This feeding and isotope protocol can be used in future studies aiming to study amino acid metabolism in cats.https://www.cambridge.org/core/product/identifier/S2048679023000447/type/journal_articleAmino acid metabolismAmino acid oxidation techniqueBicarbonate metabolismCarnivore |
| spellingShingle | Julia Guazzelli Pezzali Jocelyn G. Lambie Stuart M. Phillips Anna K. Shoveller Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat Journal of Nutritional Science Amino acid metabolism Amino acid oxidation technique Bicarbonate metabolism Carnivore |
| title | Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| title_full | Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| title_fullStr | Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| title_full_unstemmed | Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| title_short | Determination of a steady-state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| title_sort | determination of a steady state isotope dilution protocol for carbon oxidation studies in the domestic cat |
| topic | Amino acid metabolism Amino acid oxidation technique Bicarbonate metabolism Carnivore |
| url | https://www.cambridge.org/core/product/identifier/S2048679023000447/type/journal_article |
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