A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors
<p>Abstract</p> <p>Background</p> <p>Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalproceduresonline.com/content/14/1/8 |
| _version_ | 1811285181358669824 |
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| author | Uhde-Stone Claudia Huang Joseph Lu Biao |
| author_facet | Uhde-Stone Claudia Huang Joseph Lu Biao |
| author_sort | Uhde-Stone Claudia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence-specific perturbation and offers broad applications in genetic and epigenetic studies. Although the efficient construction of TALEs has been established, robust functional tools to assess their functions remain lacking.</p> <p>Results</p> <p>We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs. We validated both sensitivity and specificity of this dual-reporter system in mammalian cells, and demonstrated that this dual reporter system is robust and potentially amenable to high throughput (HTP) applications.</p> <p>Conclusion</p> <p>We have designed, constructed and validated a novel dual reporter system for assessing TALE mediated gene regulations. This system offers a robust and easy-to- use tool for real-time monitoring and quantifying gene expression in mammalian cells.</p> |
| first_indexed | 2024-04-13T02:40:33Z |
| format | Article |
| id | doaj.art-88a25127cc0345c9843c2b683266fc92 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-04-13T02:40:33Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-88a25127cc0345c9843c2b683266fc922022-12-22T03:06:13ZengBMCBiological Procedures Online1480-92222012-08-01141810.1186/1480-9222-14-8A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectorsUhde-Stone ClaudiaHuang JosephLu Biao<p>Abstract</p> <p>Background</p> <p>Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence-specific perturbation and offers broad applications in genetic and epigenetic studies. Although the efficient construction of TALEs has been established, robust functional tools to assess their functions remain lacking.</p> <p>Results</p> <p>We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs. We validated both sensitivity and specificity of this dual-reporter system in mammalian cells, and demonstrated that this dual reporter system is robust and potentially amenable to high throughput (HTP) applications.</p> <p>Conclusion</p> <p>We have designed, constructed and validated a novel dual reporter system for assessing TALE mediated gene regulations. This system offers a robust and easy-to- use tool for real-time monitoring and quantifying gene expression in mammalian cells.</p>http://www.biologicalproceduresonline.com/content/14/1/8Dual reporterGene editingTranscription activator-like effectorGreen fluorescent proteinFirefly luciferase |
| spellingShingle | Uhde-Stone Claudia Huang Joseph Lu Biao A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors Biological Procedures Online Dual reporter Gene editing Transcription activator-like effector Green fluorescent protein Firefly luciferase |
| title | A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors |
| title_full | A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors |
| title_fullStr | A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors |
| title_full_unstemmed | A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors |
| title_short | A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors |
| title_sort | robust dual reporter system to visualize and quantify gene expression mediated by transcription activator like effectors |
| topic | Dual reporter Gene editing Transcription activator-like effector Green fluorescent protein Firefly luciferase |
| url | http://www.biologicalproceduresonline.com/content/14/1/8 |
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