Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells
The erythropoietin receptor (EPOR) is a transmembrane type I receptor with an essential role in the proliferation and differentiation of erythroid progenitors. Besides its function during erythropoiesis, EPOR is expressed and has protective effect in various non-hematopoietic tissues, including tumo...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8482 |
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| author | Zuzana Tóthová Martina Šemeláková Katarína Bhide Mangesh Bhide Andrej Kováč Petra Majerová Monika Kvaková Jana Štofilová Zuzana Solárová Peter Solár |
| author_facet | Zuzana Tóthová Martina Šemeláková Katarína Bhide Mangesh Bhide Andrej Kováč Petra Majerová Monika Kvaková Jana Štofilová Zuzana Solárová Peter Solár |
| author_sort | Zuzana Tóthová |
| collection | DOAJ |
| description | The erythropoietin receptor (EPOR) is a transmembrane type I receptor with an essential role in the proliferation and differentiation of erythroid progenitors. Besides its function during erythropoiesis, EPOR is expressed and has protective effect in various non-hematopoietic tissues, including tumors. Currently, the advantageous aspect of EPOR related to different cellular events is still under scientific investigation. Besides its well-known effect on cell proliferation, apoptosis and differentiation, our integrative functional study revealed its possible associations with metabolic processes, transport of small molecules, signal transduction and tumorigenesis. Comparative transcriptome analysis (RNA-seq) identified 233 differentially expressed genes (DEGs) in EPOR overexpressed RAMA 37-28 cells compared to parental RAMA 37 cells, whereas 145 genes were downregulated and 88 upregulated. Of these, for example, <i>GPC4</i>, <i>RAP2C</i>, <i>STK26</i>, <i>ZFP955A</i>, <i>KIT</i>, <i>GAS6</i>, <i>PTPRF</i> and <i>CXCR4</i> were downregulated and <i>CDH13</i>, <i>NR0B1</i>, <i>OCM2</i>, <i>GPM6B</i>, <i>TM7SF3</i>, <i>PARVB</i>, <i>VEGFD</i> and <i>STAT5A</i> were upregulated. Surprisingly, two ephrin receptors, <i>EPHA4</i> and <i>EPHB3</i>, and <i>EFNB1</i> ligand were found to be upregulated as well. Our study is the first demonstrating robust differentially expressed genes evoked by simple EPOR overexpression without the addition of erythropoietin ligand in a manner which remains to be elucidated. |
| first_indexed | 2024-03-11T03:40:40Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:40:40Z |
| publishDate | 2023-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-88a435e5e0c34f05bc1fa1cda85257d32023-11-18T01:36:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410848210.3390/ijms24108482Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 CellsZuzana Tóthová0Martina Šemeláková1Katarína Bhide2Mangesh Bhide3Andrej Kováč4Petra Majerová5Monika Kvaková6Jana Štofilová7Zuzana Solárová8Peter Solár9Department of Medical Biology, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaDepartment of Medical Biology, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaLaboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, 04001 Košice, SlovakiaLaboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, 04001 Košice, SlovakiaInstitute of Neuroimmunology, Slovak Academy of Sciences, 84510 Bratislava, SlovakiaInstitute of Neuroimmunology, Slovak Academy of Sciences, 84510 Bratislava, SlovakiaDepartment of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaDepartment of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaDepartment of Pharmacology, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaDepartment of Medical Biology, Faculty of Medicine, P.J. Šafárik University in Košice, 04001 Košice, SlovakiaThe erythropoietin receptor (EPOR) is a transmembrane type I receptor with an essential role in the proliferation and differentiation of erythroid progenitors. Besides its function during erythropoiesis, EPOR is expressed and has protective effect in various non-hematopoietic tissues, including tumors. Currently, the advantageous aspect of EPOR related to different cellular events is still under scientific investigation. Besides its well-known effect on cell proliferation, apoptosis and differentiation, our integrative functional study revealed its possible associations with metabolic processes, transport of small molecules, signal transduction and tumorigenesis. Comparative transcriptome analysis (RNA-seq) identified 233 differentially expressed genes (DEGs) in EPOR overexpressed RAMA 37-28 cells compared to parental RAMA 37 cells, whereas 145 genes were downregulated and 88 upregulated. Of these, for example, <i>GPC4</i>, <i>RAP2C</i>, <i>STK26</i>, <i>ZFP955A</i>, <i>KIT</i>, <i>GAS6</i>, <i>PTPRF</i> and <i>CXCR4</i> were downregulated and <i>CDH13</i>, <i>NR0B1</i>, <i>OCM2</i>, <i>GPM6B</i>, <i>TM7SF3</i>, <i>PARVB</i>, <i>VEGFD</i> and <i>STAT5A</i> were upregulated. Surprisingly, two ephrin receptors, <i>EPHA4</i> and <i>EPHB3</i>, and <i>EFNB1</i> ligand were found to be upregulated as well. Our study is the first demonstrating robust differentially expressed genes evoked by simple EPOR overexpression without the addition of erythropoietin ligand in a manner which remains to be elucidated.https://www.mdpi.com/1422-0067/24/10/8482mammary adenocarcinomaRAMA 37-28erythropoietin receptor |
| spellingShingle | Zuzana Tóthová Martina Šemeláková Katarína Bhide Mangesh Bhide Andrej Kováč Petra Majerová Monika Kvaková Jana Štofilová Zuzana Solárová Peter Solár Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells International Journal of Molecular Sciences mammary adenocarcinoma RAMA 37-28 erythropoietin receptor |
| title | Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells |
| title_full | Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells |
| title_fullStr | Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells |
| title_full_unstemmed | Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells |
| title_short | Differentially Expressed Genes Induced by Erythropoietin Receptor Overexpression in Rat Mammary Adenocarcinoma RAMA 37-28 Cells |
| title_sort | differentially expressed genes induced by erythropoietin receptor overexpression in rat mammary adenocarcinoma rama 37 28 cells |
| topic | mammary adenocarcinoma RAMA 37-28 erythropoietin receptor |
| url | https://www.mdpi.com/1422-0067/24/10/8482 |
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