Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance
Antimicrobial resistance (AMR) poses a significant global health risk as a consequence of misuse of antibiotics. Owing to the increasing antimicrobial resistance, it became imperative to develop novel molecules and materials with antimicrobial properties. Porphyrins and metalloporphyrins are compoun...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/2/226 |
| _version_ | 1797298767937929216 |
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| author | Chrysanthi Pinelopi Apostolidou Chrysoula Kokotidou Varvara Platania Vasilis Nikolaou Georgios Landrou Emmanouil Nikoloudakis Georgios Charalambidis Maria Chatzinikolaidou Athanassios G. Coutsolelos Anna Mitraki |
| author_facet | Chrysanthi Pinelopi Apostolidou Chrysoula Kokotidou Varvara Platania Vasilis Nikolaou Georgios Landrou Emmanouil Nikoloudakis Georgios Charalambidis Maria Chatzinikolaidou Athanassios G. Coutsolelos Anna Mitraki |
| author_sort | Chrysanthi Pinelopi Apostolidou |
| collection | DOAJ |
| description | Antimicrobial resistance (AMR) poses a significant global health risk as a consequence of misuse of antibiotics. Owing to the increasing antimicrobial resistance, it became imperative to develop novel molecules and materials with antimicrobial properties. Porphyrins and metalloporphyrins are compounds which present antimicrobial properties especially after irradiation. As a consequence, porphyrinoids have recently been utilized as antimicrobial agents in antimicrobial photodynamic inactivation in bacteria and other microorganisms. Herein, we report the encapsulation of porphyrins into peptide hydrogels which serve as delivery vehicles. We selected the self-assembling Fmoc-Phe-Phe dipeptide, a potent gelator, as a scaffold due to its previously reported biocompatibility and three different water-soluble porphyrins as photosensitizers. We evaluated the structural, mechanical and <i>in vitro</i> degradation properties of these hydrogels, their interaction with NIH3T3 mouse skin fibroblasts, and we assessed their antimicrobial efficacy against Gram-positive <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and Gram-negative <i>Escherichia coli</i> (<i>E. coli</i>) bacteria. We found out that the hydrogels are cytocompatible and display antimicrobial efficiency against both strains with the zinc porphyrins being more efficient. Therefore, these hydrogels present a promising alternative for combating bacterial infections in the face of growing AMR concerns. |
| first_indexed | 2024-03-07T22:40:49Z |
| format | Article |
| id | doaj.art-88ad23ce0ecd4de3b8def673d0bd7752 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-07T22:40:49Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-88ad23ce0ecd4de3b8def673d0bd77522024-02-23T15:09:25ZengMDPI AGBiomolecules2218-273X2024-02-0114222610.3390/biom14020226Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial ResistanceChrysanthi Pinelopi Apostolidou0Chrysoula Kokotidou1Varvara Platania2Vasilis Nikolaou3Georgios Landrou4Emmanouil Nikoloudakis5Georgios Charalambidis6Maria Chatzinikolaidou7Athanassios G. Coutsolelos8Anna Mitraki9Department of Materials Science and Technology, University of Crete, Voutes Campus, 71003 Heraklion, GreeceDepartment of Materials Science and Technology, University of Crete, Voutes Campus, 71003 Heraklion, GreeceDepartment of Materials Science and Technology, University of Crete, Voutes Campus, 71003 Heraklion, GreeceLaboratory of Bioinorganic Chemistry, Department of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceLaboratory of Bioinorganic Chemistry, Department of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceLaboratory of Bioinorganic Chemistry, Department of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceLaboratory of Bioinorganic Chemistry, Department of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceDepartment of Materials Science and Technology, University of Crete, Voutes Campus, 71003 Heraklion, GreeceInstitute of Electronic Structure and Laser (IESL) FORTH, 70013 Heraklion, GreeceDepartment of Materials Science and Technology, University of Crete, Voutes Campus, 71003 Heraklion, GreeceAntimicrobial resistance (AMR) poses a significant global health risk as a consequence of misuse of antibiotics. Owing to the increasing antimicrobial resistance, it became imperative to develop novel molecules and materials with antimicrobial properties. Porphyrins and metalloporphyrins are compounds which present antimicrobial properties especially after irradiation. As a consequence, porphyrinoids have recently been utilized as antimicrobial agents in antimicrobial photodynamic inactivation in bacteria and other microorganisms. Herein, we report the encapsulation of porphyrins into peptide hydrogels which serve as delivery vehicles. We selected the self-assembling Fmoc-Phe-Phe dipeptide, a potent gelator, as a scaffold due to its previously reported biocompatibility and three different water-soluble porphyrins as photosensitizers. We evaluated the structural, mechanical and <i>in vitro</i> degradation properties of these hydrogels, their interaction with NIH3T3 mouse skin fibroblasts, and we assessed their antimicrobial efficacy against Gram-positive <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and Gram-negative <i>Escherichia coli</i> (<i>E. coli</i>) bacteria. We found out that the hydrogels are cytocompatible and display antimicrobial efficiency against both strains with the zinc porphyrins being more efficient. Therefore, these hydrogels present a promising alternative for combating bacterial infections in the face of growing AMR concerns.https://www.mdpi.com/2218-273X/14/2/226porphyrinshydrogelsself-assembling peptidesantimicrobial activityencapsulationcontrolled release |
| spellingShingle | Chrysanthi Pinelopi Apostolidou Chrysoula Kokotidou Varvara Platania Vasilis Nikolaou Georgios Landrou Emmanouil Nikoloudakis Georgios Charalambidis Maria Chatzinikolaidou Athanassios G. Coutsolelos Anna Mitraki Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance Biomolecules porphyrins hydrogels self-assembling peptides antimicrobial activity encapsulation controlled release |
| title | Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance |
| title_full | Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance |
| title_fullStr | Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance |
| title_full_unstemmed | Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance |
| title_short | Antimicrobial Potency of Fmoc-Phe-Phe Dipeptide Hydrogels with Encapsulated Porphyrin Chromophores Is a Promising Alternative in Antimicrobial Resistance |
| title_sort | antimicrobial potency of fmoc phe phe dipeptide hydrogels with encapsulated porphyrin chromophores is a promising alternative in antimicrobial resistance |
| topic | porphyrins hydrogels self-assembling peptides antimicrobial activity encapsulation controlled release |
| url | https://www.mdpi.com/2218-273X/14/2/226 |
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