In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis
Oral candidiasis presents with multiple clinical manifestations. Among known pathogenic <i>Candida</i> species, <i>Candida albicans</i> is the most virulent and acts as the main causative fungus of oral candidiasis. Novel treatment modalities are needed because of emergent dr...
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| Materyal Türü: | Makale |
| Dil: | English |
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MDPI AG
2021-04-01
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| Seri Bilgileri: | Journal of Fungi |
| Konular: | |
| Online Erişim: | https://www.mdpi.com/2309-608X/7/5/322 |
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| author | Rei Ito Yuichi Mine Yoshie Yumisashi Reina Yoshioka Misa Hamaoka Tsuyoshi Taji Takeshi Murayama Hiroki Nikawa |
| author_facet | Rei Ito Yuichi Mine Yoshie Yumisashi Reina Yoshioka Misa Hamaoka Tsuyoshi Taji Takeshi Murayama Hiroki Nikawa |
| author_sort | Rei Ito |
| collection | DOAJ |
| description | Oral candidiasis presents with multiple clinical manifestations. Among known pathogenic <i>Candida</i> species, <i>Candida albicans</i> is the most virulent and acts as the main causative fungus of oral candidiasis. Novel treatment modalities are needed because of emergent drug resistance and frequent candidiasis recurrence. Here, we evaluated the ability of <i>Lacticaseibacillus rhamnosus</i> L8020, isolated from healthy and caries-free volunteers, to prevent against the onset of oral candidiasis in a mouse model. Mice were infected with <i>C. albicans</i>, in the presence or absence of <i>L. rhamnosus</i> L8020. The mice were treated with antibiotics and corticosteroid to disrupt the oral microbiota and induce immunosuppression. We demonstrated that oral consumption of <i>L. rhamnosus</i> L8020 by <i>C. albicans</i>-infected mice abolished the pseudomembranous region of the mouse tongue; it also suppressed changes in the expression levels of pattern recognition receptor and chemokine genes. Our results suggest that <i>L. rhamnosus</i> L8020 has protective or therapeutic potential against oral candidiasis, which supports the potential use of this probiotic strain for oral health management. |
| first_indexed | 2024-03-10T12:07:43Z |
| format | Article |
| id | doaj.art-88adba1a5a6f43a883465c3669e4dca8 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T12:07:43Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-88adba1a5a6f43a883465c3669e4dca82023-11-21T16:26:29ZengMDPI AGJournal of Fungi2309-608X2021-04-017532210.3390/jof7050322In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral CandidiasisRei Ito0Yuichi Mine1Yoshie Yumisashi2Reina Yoshioka3Misa Hamaoka4Tsuyoshi Taji5Takeshi Murayama6Hiroki Nikawa7Department of Oral Biology & Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Medical System Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Biology & Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Medical System Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Biology & Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Biology & Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Medical System Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Biology & Engineering, Division of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanOral candidiasis presents with multiple clinical manifestations. Among known pathogenic <i>Candida</i> species, <i>Candida albicans</i> is the most virulent and acts as the main causative fungus of oral candidiasis. Novel treatment modalities are needed because of emergent drug resistance and frequent candidiasis recurrence. Here, we evaluated the ability of <i>Lacticaseibacillus rhamnosus</i> L8020, isolated from healthy and caries-free volunteers, to prevent against the onset of oral candidiasis in a mouse model. Mice were infected with <i>C. albicans</i>, in the presence or absence of <i>L. rhamnosus</i> L8020. The mice were treated with antibiotics and corticosteroid to disrupt the oral microbiota and induce immunosuppression. We demonstrated that oral consumption of <i>L. rhamnosus</i> L8020 by <i>C. albicans</i>-infected mice abolished the pseudomembranous region of the mouse tongue; it also suppressed changes in the expression levels of pattern recognition receptor and chemokine genes. Our results suggest that <i>L. rhamnosus</i> L8020 has protective or therapeutic potential against oral candidiasis, which supports the potential use of this probiotic strain for oral health management.https://www.mdpi.com/2309-608X/7/5/322oral candidiasis<i>Candida albicans</i>probiotics<i>Lacticaseibacillus rhamnosus</i> L8020 |
| spellingShingle | Rei Ito Yuichi Mine Yoshie Yumisashi Reina Yoshioka Misa Hamaoka Tsuyoshi Taji Takeshi Murayama Hiroki Nikawa In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis Journal of Fungi oral candidiasis <i>Candida albicans</i> probiotics <i>Lacticaseibacillus rhamnosus</i> L8020 |
| title | In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis |
| title_full | In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis |
| title_fullStr | In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis |
| title_full_unstemmed | In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis |
| title_short | In Vivo Efficacy of <i>Lacticaseibacillus rhamnosus</i> L8020 in a Mouse Model of Oral Candidiasis |
| title_sort | in vivo efficacy of i lacticaseibacillus rhamnosus i l8020 in a mouse model of oral candidiasis |
| topic | oral candidiasis <i>Candida albicans</i> probiotics <i>Lacticaseibacillus rhamnosus</i> L8020 |
| url | https://www.mdpi.com/2309-608X/7/5/322 |
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