Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System
An auto-inducing expression system was developed that could express target genes in <i>S. marcescens</i> MG1. Using this system, MG1 was constructed as a whole-cell biocatalyst to produce 2,3-butanediol/acetoin. Formate dehydrogenase (FDH) and 2,3-butanediol dehydrogenase were expressed...
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MDPI AG
2021-11-01
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| author | Yaping Wang Yanhong Peng Xiaoyan Liu Ronghua Zhou Xianqing Liao Yong Min Yong Hu Ying Wang Ben Rao |
| author_facet | Yaping Wang Yanhong Peng Xiaoyan Liu Ronghua Zhou Xianqing Liao Yong Min Yong Hu Ying Wang Ben Rao |
| author_sort | Yaping Wang |
| collection | DOAJ |
| description | An auto-inducing expression system was developed that could express target genes in <i>S. marcescens</i> MG1. Using this system, MG1 was constructed as a whole-cell biocatalyst to produce 2,3-butanediol/acetoin. Formate dehydrogenase (FDH) and 2,3-butanediol dehydrogenase were expressed together to build an NADH regeneration system to transform diacetyl to 2,3-butanediol. After fermentation, the extract of recombinant <i>S. marcescens</i> MG1ABC <i>(pETDuet-bdhA-fdh)</i> showed 2,3-BDH activity of 57.8 U/mg and FDH activity of 0.5 U/mg. And 27.95 g/L of 2,3-BD was achieved with a productivity of 4.66 g/Lh using engineered <i>S. marcescens</i> MG1<i>(Pswnb+pETDuet-bdhA-fdh)</i> after 6 h incubation. Next, to produce 2,3-butanediol from acetoin, NADH oxidase and 2,3-butanediol dehydrogenase from <i>Bacillus subtilis</i> were co-expressed to obtain a NAD+ regeneration system. After fermentation, the recombinant strain <i>S. marcescens</i> MG1ABC <i>(pSWNB+pETDuet-bdhA-yodC)</i> showed AR activity of 212.4 U/mg and NOX activity of 150.1 U/mg. We obtained 44.9 g/L of acetoin with a productivity of 3.74 g/Lh using <i>S. marcescens</i> MG1ABC (<i>pSWNB+pETDuet-bdhA-yodC</i>). This work confirmed that <i>S. marcescens</i> could be designed as a whole-cell biocatalyst for 2,3-butanediol and acetoin production. |
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| language | English |
| last_indexed | 2024-03-10T04:30:56Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-88af13cf570c4cde960a8e824b832b8b2023-11-23T04:08:41ZengMDPI AGCatalysts2073-43442021-11-011112142210.3390/catal11121422Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration SystemYaping Wang0Yanhong Peng1Xiaoyan Liu2Ronghua Zhou3Xianqing Liao4Yong Min5Yong Hu6Ying Wang7Ben Rao8National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan 430068, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, ChinaAn auto-inducing expression system was developed that could express target genes in <i>S. marcescens</i> MG1. Using this system, MG1 was constructed as a whole-cell biocatalyst to produce 2,3-butanediol/acetoin. Formate dehydrogenase (FDH) and 2,3-butanediol dehydrogenase were expressed together to build an NADH regeneration system to transform diacetyl to 2,3-butanediol. After fermentation, the extract of recombinant <i>S. marcescens</i> MG1ABC <i>(pETDuet-bdhA-fdh)</i> showed 2,3-BDH activity of 57.8 U/mg and FDH activity of 0.5 U/mg. And 27.95 g/L of 2,3-BD was achieved with a productivity of 4.66 g/Lh using engineered <i>S. marcescens</i> MG1<i>(Pswnb+pETDuet-bdhA-fdh)</i> after 6 h incubation. Next, to produce 2,3-butanediol from acetoin, NADH oxidase and 2,3-butanediol dehydrogenase from <i>Bacillus subtilis</i> were co-expressed to obtain a NAD+ regeneration system. After fermentation, the recombinant strain <i>S. marcescens</i> MG1ABC <i>(pSWNB+pETDuet-bdhA-yodC)</i> showed AR activity of 212.4 U/mg and NOX activity of 150.1 U/mg. We obtained 44.9 g/L of acetoin with a productivity of 3.74 g/Lh using <i>S. marcescens</i> MG1ABC (<i>pSWNB+pETDuet-bdhA-yodC</i>). This work confirmed that <i>S. marcescens</i> could be designed as a whole-cell biocatalyst for 2,3-butanediol and acetoin production.https://www.mdpi.com/2073-4344/11/12/14222,3-butanediolacetoin<i>Serratia marcescens</i>whole-cell biocatalystsNADH/NAD<sup>+</sup> regeneration system |
| spellingShingle | Yaping Wang Yanhong Peng Xiaoyan Liu Ronghua Zhou Xianqing Liao Yong Min Yong Hu Ying Wang Ben Rao Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System Catalysts 2,3-butanediol acetoin <i>Serratia marcescens</i> whole-cell biocatalysts NADH/NAD<sup>+</sup> regeneration system |
| title | Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System |
| title_full | Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System |
| title_fullStr | Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System |
| title_full_unstemmed | Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System |
| title_short | Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System |
| title_sort | efficient 2 3 butanediol acetoin production using whole cell biocatalyst with a new nadh nad regeneration system |
| topic | 2,3-butanediol acetoin <i>Serratia marcescens</i> whole-cell biocatalysts NADH/NAD<sup>+</sup> regeneration system |
| url | https://www.mdpi.com/2073-4344/11/12/1422 |
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