Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes
Wheat bran fibers are considered beneficial to human health through their impact on gut microbiota composition and activity. Here, we assessed the prebiotic potential of selected bran fractions by performing a series of fecal slurry anaerobic fermentation experiments using aleurone as well as total,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00031/full |
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| author | Kevin D’hoe Kevin D’hoe Kevin D’hoe Lorenza Conterno Lorenza Conterno Francesca Fava Gwen Falony Gwen Falony Sara Vieira-Silva Sara Vieira-Silva Joan Vermeiren Kieran Tuohy Jeroen Raes Jeroen Raes Jeroen Raes |
| author_facet | Kevin D’hoe Kevin D’hoe Kevin D’hoe Lorenza Conterno Lorenza Conterno Francesca Fava Gwen Falony Gwen Falony Sara Vieira-Silva Sara Vieira-Silva Joan Vermeiren Kieran Tuohy Jeroen Raes Jeroen Raes Jeroen Raes |
| author_sort | Kevin D’hoe |
| collection | DOAJ |
| description | Wheat bran fibers are considered beneficial to human health through their impact on gut microbiota composition and activity. Here, we assessed the prebiotic potential of selected bran fractions by performing a series of fecal slurry anaerobic fermentation experiments using aleurone as well as total, ultrafine, and soluble wheat bran (swb) as carbon sources. By combining amplicon-based community profiling with a fluorescent in situ hybridization (FISH) approach, we found that incubation conditions favor the growth of Proteobacteria such as Escherichia and Bilophila. These effects were countered in all but one [total wheat bran (twb)] fermentation experiments. Growth of Bifidobacterium species was stimulated after fermentation using ultrafine, soluble, and twb, in the latter two as part of a general increase in bacterial load. Both ultrafine and swb fermentation resulted in a trade-off between Bifidobacterium and Bilophila, as previously observed in human dietary supplementation studies looking at the effect of inulin-type fructans on the human gut microbiota. Aleurone selectively stimulated growth of Dorea and butyrate-producing Roseburia. All fermentation experiments induced enhanced gas production; increased butyrate concentrations were only observed following soluble bran incubation. Our results open perspectives for the development of aleurone as a complementary prebiotic selectively targeting colon butyrate producers. |
| first_indexed | 2024-12-13T16:42:14Z |
| format | Article |
| id | doaj.art-88b24f8657fc4291863a65c4727b90f7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T16:42:14Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-88b24f8657fc4291863a65c4727b90f72022-12-21T23:38:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01910.3389/fmicb.2018.00031322581Prebiotic Wheat Bran Fractions Induce Specific Microbiota ChangesKevin D’hoe0Kevin D’hoe1Kevin D’hoe2Lorenza Conterno3Lorenza Conterno4Francesca Fava5Gwen Falony6Gwen Falony7Sara Vieira-Silva8Sara Vieira-Silva9Joan Vermeiren10Kieran Tuohy11Jeroen Raes12Jeroen Raes13Jeroen Raes14Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, BelgiumJeroen Raes Lab, VIB KU Leuven Center for Microbiology, Leuven, BelgiumResearch Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, BelgiumDepartment of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, Trento, ItalyFermentation and Distillation, Laimburg Research Centre, Bolzano, ItalyDepartment of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, Trento, ItalyLaboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, BelgiumJeroen Raes Lab, VIB KU Leuven Center for Microbiology, Leuven, BelgiumLaboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, BelgiumJeroen Raes Lab, VIB KU Leuven Center for Microbiology, Leuven, BelgiumCargill R&D Centre Europe BVBA, Vilvoorde, BelgiumDepartment of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, Trento, ItalyLaboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, BelgiumJeroen Raes Lab, VIB KU Leuven Center for Microbiology, Leuven, BelgiumResearch Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, BelgiumWheat bran fibers are considered beneficial to human health through their impact on gut microbiota composition and activity. Here, we assessed the prebiotic potential of selected bran fractions by performing a series of fecal slurry anaerobic fermentation experiments using aleurone as well as total, ultrafine, and soluble wheat bran (swb) as carbon sources. By combining amplicon-based community profiling with a fluorescent in situ hybridization (FISH) approach, we found that incubation conditions favor the growth of Proteobacteria such as Escherichia and Bilophila. These effects were countered in all but one [total wheat bran (twb)] fermentation experiments. Growth of Bifidobacterium species was stimulated after fermentation using ultrafine, soluble, and twb, in the latter two as part of a general increase in bacterial load. Both ultrafine and swb fermentation resulted in a trade-off between Bifidobacterium and Bilophila, as previously observed in human dietary supplementation studies looking at the effect of inulin-type fructans on the human gut microbiota. Aleurone selectively stimulated growth of Dorea and butyrate-producing Roseburia. All fermentation experiments induced enhanced gas production; increased butyrate concentrations were only observed following soluble bran incubation. Our results open perspectives for the development of aleurone as a complementary prebiotic selectively targeting colon butyrate producers.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00031/fullwheat branaleuroneprebioticin vitrofermentationmicrobiome |
| spellingShingle | Kevin D’hoe Kevin D’hoe Kevin D’hoe Lorenza Conterno Lorenza Conterno Francesca Fava Gwen Falony Gwen Falony Sara Vieira-Silva Sara Vieira-Silva Joan Vermeiren Kieran Tuohy Jeroen Raes Jeroen Raes Jeroen Raes Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes Frontiers in Microbiology wheat bran aleurone prebiotic in vitro fermentation microbiome |
| title | Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes |
| title_full | Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes |
| title_fullStr | Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes |
| title_full_unstemmed | Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes |
| title_short | Prebiotic Wheat Bran Fractions Induce Specific Microbiota Changes |
| title_sort | prebiotic wheat bran fractions induce specific microbiota changes |
| topic | wheat bran aleurone prebiotic in vitro fermentation microbiome |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00031/full |
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