Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating
The resistance of microorganisms to antibiotics is a crucial problem for which the application of nanomaterials is among a growing number of solutions. The aim of the study was to create a nanocomposite (composed of graphene oxide and silver nanoparticles) with a precise mode of antibacterial action...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/15/9/3122 |
| _version_ | 1797504017743478784 |
|---|---|
| author | Agata Lange Ewa Sawosz Mateusz Wierzbicki Marta Kutwin Karolina Daniluk Barbara Strojny Agnieszka Ostrowska Barbara Wójcik Maciej Łojkowski Marcin Gołębiewski André Chwalibog Sławomir Jaworski |
| author_facet | Agata Lange Ewa Sawosz Mateusz Wierzbicki Marta Kutwin Karolina Daniluk Barbara Strojny Agnieszka Ostrowska Barbara Wójcik Maciej Łojkowski Marcin Gołębiewski André Chwalibog Sławomir Jaworski |
| author_sort | Agata Lange |
| collection | DOAJ |
| description | The resistance of microorganisms to antibiotics is a crucial problem for which the application of nanomaterials is among a growing number of solutions. The aim of the study was to create a nanocomposite (composed of graphene oxide and silver nanoparticles) with a precise mode of antibacterial action: what enables textiles to be coated in order to exhibit antibacterial properties. A characterization of nanomaterials (silver nanoparticles and graphene oxide) by size distribution, zeta potential measurements, TEM visualization and FT-IR was performed. The biological studies of the nanocomposite and its components included the toxicity effect toward two pathogenic bacteria species, namely <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>, interaction of nanomaterials with the outer layer of microorganisms, and the generation of reactive oxygen species and lipid peroxidation. Afterwards, antibacterial studies of the nanocomposite’s coated textiles (cotton, interlining fabric, polypropylene and silk) as well as studies of the general toxicity towards a chicken embryo chorioallantoic membrane model were conducted. The toxicity of the nanocomposite used was higher than its components applied separately (zones of growth inhibition for <i>P. aeruginosa</i> for the final selected concentrations were as follows: silver nanoparticles 21 ± 0.7 mm, graphene oxide 14 ± 1.9 mm and nanocomposite 23 ± 1.6 mm; and for <i>S. aureus</i> were: silver nanoparticles 27 ± 3.8 mm, graphene oxide 14 ± 2.1 mm, and nanocomposite 28 ± 0.4 mm. The viability of <i>P. aeruginosa</i> and <i>S. aureus</i> after treatment with selected GO-Ag decreased to 27% and 31%, respectively, compared to AgNPs, when the viability of both species was 31% and 34%, accordingly). The coated textiles showed encouraging antibacterial features without general toxicity towards the chicken embryo chorioallantoic membrane model. We demonstrated that graphene oxide might constitute a functional platform for silver nanoparticles, improving the antibacterial properties of bare silver. Due to the application of the nanocomposite, the textiles showed promising antibacterial features with a low general toxicity, thereby creating a wide possibility for them to be used in practice. |
| first_indexed | 2024-03-10T03:58:37Z |
| format | Article |
| id | doaj.art-88b263af3a6e4dcbbaccc1a3f03604b8 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T03:58:37Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-88b263af3a6e4dcbbaccc1a3f03604b82023-11-23T08:38:32ZengMDPI AGMaterials1996-19442022-04-01159312210.3390/ma15093122Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles CoatingAgata Lange0Ewa Sawosz1Mateusz Wierzbicki2Marta Kutwin3Karolina Daniluk4Barbara Strojny5Agnieszka Ostrowska6Barbara Wójcik7Maciej Łojkowski8Marcin Gołębiewski9André Chwalibog10Sławomir Jaworski11Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandFaculty of Materials Science and Engineering, Warsaw University of Technology, Wołoska 141 Str., 02-507 Warsaw, PolandDepartment of Animal Breeding, Institute of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandDepartment of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 3 Str., 1870 Frederiksberg, DenmarkDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 Str., 02-786 Warsaw, PolandThe resistance of microorganisms to antibiotics is a crucial problem for which the application of nanomaterials is among a growing number of solutions. The aim of the study was to create a nanocomposite (composed of graphene oxide and silver nanoparticles) with a precise mode of antibacterial action: what enables textiles to be coated in order to exhibit antibacterial properties. A characterization of nanomaterials (silver nanoparticles and graphene oxide) by size distribution, zeta potential measurements, TEM visualization and FT-IR was performed. The biological studies of the nanocomposite and its components included the toxicity effect toward two pathogenic bacteria species, namely <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>, interaction of nanomaterials with the outer layer of microorganisms, and the generation of reactive oxygen species and lipid peroxidation. Afterwards, antibacterial studies of the nanocomposite’s coated textiles (cotton, interlining fabric, polypropylene and silk) as well as studies of the general toxicity towards a chicken embryo chorioallantoic membrane model were conducted. The toxicity of the nanocomposite used was higher than its components applied separately (zones of growth inhibition for <i>P. aeruginosa</i> for the final selected concentrations were as follows: silver nanoparticles 21 ± 0.7 mm, graphene oxide 14 ± 1.9 mm and nanocomposite 23 ± 1.6 mm; and for <i>S. aureus</i> were: silver nanoparticles 27 ± 3.8 mm, graphene oxide 14 ± 2.1 mm, and nanocomposite 28 ± 0.4 mm. The viability of <i>P. aeruginosa</i> and <i>S. aureus</i> after treatment with selected GO-Ag decreased to 27% and 31%, respectively, compared to AgNPs, when the viability of both species was 31% and 34%, accordingly). The coated textiles showed encouraging antibacterial features without general toxicity towards the chicken embryo chorioallantoic membrane model. We demonstrated that graphene oxide might constitute a functional platform for silver nanoparticles, improving the antibacterial properties of bare silver. Due to the application of the nanocomposite, the textiles showed promising antibacterial features with a low general toxicity, thereby creating a wide possibility for them to be used in practice.https://www.mdpi.com/1996-1944/15/9/3122nanocompositesilver nanoparticlesgraphene oxideantibacterialcoating |
| spellingShingle | Agata Lange Ewa Sawosz Mateusz Wierzbicki Marta Kutwin Karolina Daniluk Barbara Strojny Agnieszka Ostrowska Barbara Wójcik Maciej Łojkowski Marcin Gołębiewski André Chwalibog Sławomir Jaworski Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating Materials nanocomposite silver nanoparticles graphene oxide antibacterial coating |
| title | Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating |
| title_full | Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating |
| title_fullStr | Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating |
| title_full_unstemmed | Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating |
| title_short | Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating |
| title_sort | nanocomposites of graphene oxide silver nanoparticles for enhanced antibacterial activity mechanism of action and medical textiles coating |
| topic | nanocomposite silver nanoparticles graphene oxide antibacterial coating |
| url | https://www.mdpi.com/1996-1944/15/9/3122 |
| work_keys_str_mv | AT agatalange nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT ewasawosz nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT mateuszwierzbicki nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT martakutwin nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT karolinadaniluk nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT barbarastrojny nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT agnieszkaostrowska nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT barbarawojcik nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT maciejłojkowski nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT marcingołebiewski nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT andrechwalibog nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating AT sławomirjaworski nanocompositesofgrapheneoxidesilvernanoparticlesforenhancedantibacterialactivitymechanismofactionandmedicaltextilescoating |