Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i>
The fish gill is the first tissue that is exposed to the external media and undergoes continuous osmotic challenges. Recently, our group published an article entitled “Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka” in the journal mSy...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/10/1528 |
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| author | Keng Po Lai Delbert Almerick T. Boncan Lu Yang Cherry Chi Tim Leung Jeff Cheuk Hin Ho Xiao Lin Ting Fung Chan Richard Yuen Chong Kong William Ka Fai Tse |
| author_facet | Keng Po Lai Delbert Almerick T. Boncan Lu Yang Cherry Chi Tim Leung Jeff Cheuk Hin Ho Xiao Lin Ting Fung Chan Richard Yuen Chong Kong William Ka Fai Tse |
| author_sort | Keng Po Lai |
| collection | DOAJ |
| description | The fish gill is the first tissue that is exposed to the external media and undergoes continuous osmotic challenges. Recently, our group published an article entitled “Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka” in the journal mSystems (e0004722, 2022), and suggested the possible host-bacterium interaction in the fish gill during osmotic stress. The previous study was performed by the progressive fresh water transfer (i.e., seawater to fresh water transfer via 50% seawater (FW)). Our group hypothesized that osmotic gradient could be a factor that determines the microbiota communities in the gill. The current 16S rRNA metagenomic sequencing study found that the direct transfer (i.e., seawater to fresh water (FWd)) could result in different gill microbiota communities in the same fresh water endpoints. <i>Pseduomonas</i> was the dominant bacteria (more than 55%) in the FWd gill. The Kyoto Encyclopedia of Genes and Genomes and MetaCyc analysis further suggested that the FWd group had enhanced osmosensing pathways, such as the ATP-binding cassette transporters, taurine degradation, and energy-related tricarboxylic acid metabolism compared to the FW group. |
| first_indexed | 2024-03-09T20:39:57Z |
| format | Article |
| id | doaj.art-88b602ea37f44f05ba13d438c964c3bc |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T20:39:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-88b602ea37f44f05ba13d438c964c3bc2023-11-23T23:01:04ZengMDPI AGBiology2079-77372022-10-011110152810.3390/biology11101528Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i>Keng Po Lai0Delbert Almerick T. Boncan1Lu Yang2Cherry Chi Tim Leung3Jeff Cheuk Hin Ho4Xiao Lin5Ting Fung Chan6Richard Yuen Chong Kong7William Ka Fai Tse8Key Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, ChinaKey Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, ChinaDepartment of Chemistry, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Chemistry, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USAState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, ChinaDepartment of Chemistry, City University of Hong Kong, Hong Kong SAR, ChinaLaboratory of Developmental Disorders and Toxicology, Center for Promotion of International Education and Research, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, JapanThe fish gill is the first tissue that is exposed to the external media and undergoes continuous osmotic challenges. Recently, our group published an article entitled “Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka” in the journal mSystems (e0004722, 2022), and suggested the possible host-bacterium interaction in the fish gill during osmotic stress. The previous study was performed by the progressive fresh water transfer (i.e., seawater to fresh water transfer via 50% seawater (FW)). Our group hypothesized that osmotic gradient could be a factor that determines the microbiota communities in the gill. The current 16S rRNA metagenomic sequencing study found that the direct transfer (i.e., seawater to fresh water (FWd)) could result in different gill microbiota communities in the same fresh water endpoints. <i>Pseduomonas</i> was the dominant bacteria (more than 55%) in the FWd gill. The Kyoto Encyclopedia of Genes and Genomes and MetaCyc analysis further suggested that the FWd group had enhanced osmosensing pathways, such as the ATP-binding cassette transporters, taurine degradation, and energy-related tricarboxylic acid metabolism compared to the FW group.https://www.mdpi.com/2079-7737/11/10/1528osmotic stressfish gill16S rRNA metagenomic sequencingstress |
| spellingShingle | Keng Po Lai Delbert Almerick T. Boncan Lu Yang Cherry Chi Tim Leung Jeff Cheuk Hin Ho Xiao Lin Ting Fung Chan Richard Yuen Chong Kong William Ka Fai Tse Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> Biology osmotic stress fish gill 16S rRNA metagenomic sequencing stress |
| title | Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> |
| title_full | Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> |
| title_fullStr | Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> |
| title_full_unstemmed | Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> |
| title_short | Osmotic Gradient Is a Factor That Influences the Gill Microbiota Communities in <i>Oryzias melastigma</i> |
| title_sort | osmotic gradient is a factor that influences the gill microbiota communities in i oryzias melastigma i |
| topic | osmotic stress fish gill 16S rRNA metagenomic sequencing stress |
| url | https://www.mdpi.com/2079-7737/11/10/1528 |
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