Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes
Natural biometabolites of plants have been reported to be useful in chronic diseases including diabetes and associated complications. This research is aimed to investigate how the biometabolites of <i>Lasia spinosa</i> methanol stem (ME<sub>X</sub>LS) extract ameliorative dia...
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MDPI AG
2022-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/12/1466 |
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| author | Md. Mamunur Rashid Md. Atiar Rahman Md. Shahidul Islam Md. Amjad Hossen A. M. Abu Ahmed Mirola Afroze Alaa H. Habib Manal M. S. Mansoury Hend F. Alharbi Reham M. Algheshairy Walla Alelwani Afnan M. Alnajeebi Jitbanjong Tangpong Srabonti Saha Alaa Qadhi Wedad Azhar |
| author_facet | Md. Mamunur Rashid Md. Atiar Rahman Md. Shahidul Islam Md. Amjad Hossen A. M. Abu Ahmed Mirola Afroze Alaa H. Habib Manal M. S. Mansoury Hend F. Alharbi Reham M. Algheshairy Walla Alelwani Afnan M. Alnajeebi Jitbanjong Tangpong Srabonti Saha Alaa Qadhi Wedad Azhar |
| author_sort | Md. Mamunur Rashid |
| collection | DOAJ |
| description | Natural biometabolites of plants have been reported to be useful in chronic diseases including diabetes and associated complications. This research is aimed to investigate how the biometabolites of <i>Lasia spinosa</i> methanol stem (ME<sub>X</sub>LS) extract ameliorative diabetes and diabetes-related complications. ME<sub>X</sub>LS was examined for in vitro antioxidant and in vivo antidiabetic effects in a streptozotocin-induced diabetes model, and its chemical profiling was done by gas chromatography-mass spectrometry analysis. The results were verified by histopathological examination and in silico ligand-receptor interaction of characterized natural biometabolites with antidiabetic receptor proteins AMPK (PDB ID: 4CFH); PPARγ (PDB ID: 3G9E); and mammalian α-amylase center (PDB ID: 1PPI). The ME<sub>X</sub>LS was found to show a remarkable α-amylase inhibition (47.45%), strong antioxidant action, and significant (<i>p</i> < 0.05) decrease in blood glucose level, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein (LDL), urea, uric acid, creatinine, total cholesterol, triglyceride (TG), liver glycogen, creatinine kinase (CK-MB), and lactate dehydrogenase (LDH) and increase in serum insulin, glucose tolerance, and high-density lipoprotein (HDL). Rat’s pancreas and kidney tissues were found to be partially recovered in histopathological analyses. Methyl α-<span style="font-variant: small-caps;">d</span><span style="font-variant: small-caps;">-</span>galactopyranoside displayed the highest binding affinity with AMPK (docking score, −5.764), PPARγ (docking score, −5.218), and 1PPI (docking score, −5.615) receptors. Data suggest that the ME<sub>X</sub>LS may be an exciting source to potentiate antidiabetic activities affirming a cell-line study. |
| first_indexed | 2024-03-09T15:59:27Z |
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| id | doaj.art-88b876c5483a4cb3960f1ceb4eaa9089 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T15:59:27Z |
| publishDate | 2022-11-01 |
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| series | Pharmaceuticals |
| spelling | doaj.art-88b876c5483a4cb3960f1ceb4eaa90892023-11-24T17:15:40ZengMDPI AGPharmaceuticals1424-82472022-11-011512146610.3390/ph15121466Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular IndexesMd. Mamunur Rashid0Md. Atiar Rahman1Md. Shahidul Islam2Md. Amjad Hossen3A. M. Abu Ahmed4Mirola Afroze5Alaa H. Habib6Manal M. S. Mansoury7Hend F. Alharbi8Reham M. Algheshairy9Walla Alelwani10Afnan M. Alnajeebi11Jitbanjong Tangpong12Srabonti Saha13Alaa Qadhi14Wedad Azhar15Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshDepartment of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong 4318, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong 4331, BangladeshBangladesh Reference Institute for Chemical Measurements (BRiCM), Dr. Qudrat-e-Khuda Road (Laboratory Road), Dhanmondi, Dhaka 1205, BangladeshDepartment of Physiology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Food and Nutrition, Faculty of Human Sciences and Design, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Biochemistry, Collage of Science, University of Jeddah, Jeddah 80203, Saudi ArabiaDepartment of Biochemistry, Collage of Science, University of Jeddah, Jeddah 80203, Saudi ArabiaSchool of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, BangladeshClinical Nutrition Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, P.O. Box 715, Makkah 21955, Saudi ArabiaClinical Nutrition Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, P.O. Box 715, Makkah 21955, Saudi ArabiaNatural biometabolites of plants have been reported to be useful in chronic diseases including diabetes and associated complications. This research is aimed to investigate how the biometabolites of <i>Lasia spinosa</i> methanol stem (ME<sub>X</sub>LS) extract ameliorative diabetes and diabetes-related complications. ME<sub>X</sub>LS was examined for in vitro antioxidant and in vivo antidiabetic effects in a streptozotocin-induced diabetes model, and its chemical profiling was done by gas chromatography-mass spectrometry analysis. The results were verified by histopathological examination and in silico ligand-receptor interaction of characterized natural biometabolites with antidiabetic receptor proteins AMPK (PDB ID: 4CFH); PPARγ (PDB ID: 3G9E); and mammalian α-amylase center (PDB ID: 1PPI). The ME<sub>X</sub>LS was found to show a remarkable α-amylase inhibition (47.45%), strong antioxidant action, and significant (<i>p</i> < 0.05) decrease in blood glucose level, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein (LDL), urea, uric acid, creatinine, total cholesterol, triglyceride (TG), liver glycogen, creatinine kinase (CK-MB), and lactate dehydrogenase (LDH) and increase in serum insulin, glucose tolerance, and high-density lipoprotein (HDL). Rat’s pancreas and kidney tissues were found to be partially recovered in histopathological analyses. Methyl α-<span style="font-variant: small-caps;">d</span><span style="font-variant: small-caps;">-</span>galactopyranoside displayed the highest binding affinity with AMPK (docking score, −5.764), PPARγ (docking score, −5.218), and 1PPI (docking score, −5.615) receptors. Data suggest that the ME<sub>X</sub>LS may be an exciting source to potentiate antidiabetic activities affirming a cell-line study.https://www.mdpi.com/1424-8247/15/12/1466<i>Lasia spinosa</i>diabetes mellitusAMPKPPARγMethyl α-<span style="font-variant: small-caps;">d-</span>galactopyranosidemethyl α-<span style="font-variant: small-caps;">d-</span>glucopyranoside |
| spellingShingle | Md. Mamunur Rashid Md. Atiar Rahman Md. Shahidul Islam Md. Amjad Hossen A. M. Abu Ahmed Mirola Afroze Alaa H. Habib Manal M. S. Mansoury Hend F. Alharbi Reham M. Algheshairy Walla Alelwani Afnan M. Alnajeebi Jitbanjong Tangpong Srabonti Saha Alaa Qadhi Wedad Azhar Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes Pharmaceuticals <i>Lasia spinosa</i> diabetes mellitus AMPK PPARγ Methyl α-<span style="font-variant: small-caps;">d-</span>galactopyranoside methyl α-<span style="font-variant: small-caps;">d-</span>glucopyranoside |
| title | Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes |
| title_full | Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes |
| title_fullStr | Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes |
| title_full_unstemmed | Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes |
| title_short | Natural Compounds of <em>Lasia spinosa</em> (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes |
| title_sort | natural compounds of em lasia spinosa em l stem potentiate antidiabetic actions by regulating diabetes and diabetes related biochemical and cellular indexes |
| topic | <i>Lasia spinosa</i> diabetes mellitus AMPK PPARγ Methyl α-<span style="font-variant: small-caps;">d-</span>galactopyranoside methyl α-<span style="font-variant: small-caps;">d-</span>glucopyranoside |
| url | https://www.mdpi.com/1424-8247/15/12/1466 |
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