Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones.
N-methyl-D-aspartate receptors (NMDARs) in cerebellar molecular layer interneurones (MLIs) are expressed and activated in unusual ways: at parallel fibre synapses they are only recruited by repetitive stimuli, suggesting an extrasynaptic location, whereas their activation by climbing fibre is purely...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-02-01
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| Series: | Frontiers in Synaptic Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnsyn.2015.00001/full |
| _version_ | 1818156618840276992 |
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| author | Celine eBidoret Celine eBidoret Guy eBouvier Annick eAyon German eSzapiro Mariano eCasado |
| author_facet | Celine eBidoret Celine eBidoret Guy eBouvier Annick eAyon German eSzapiro Mariano eCasado |
| author_sort | Celine eBidoret |
| collection | DOAJ |
| description | N-methyl-D-aspartate receptors (NMDARs) in cerebellar molecular layer interneurones (MLIs) are expressed and activated in unusual ways: at parallel fibre synapses they are only recruited by repetitive stimuli, suggesting an extrasynaptic location, whereas their activation by climbing fibre is purely mediated by spillover. NMDARs are thought to play an important role in plasticity at different levels of the cerebellar circuitry. Evaluation of the location, functional properties and physiological roles of NMDARs will be facilitated by knowledge of the NMDAR isoforms recruited. Here we show that MLI-NMDARs activated by both parallel fibre and climbing fibre inputs have similar kinetics and contain GluN2B but not GluN2A subunits. On the other hand, no evidence was found of functional NMDARs in the axons of MLIs. At the parallel fibre - Purkinje cell (PF-PC) synapse, the activation of GluN2A-containing NMDARs has been shown to be necessary for the induction of long-term depression. Our results therefore provide a clear distinction between the NMDARs located on MLIs and those involved in plasticity at PF-PC synapses. |
| first_indexed | 2024-12-11T15:01:10Z |
| format | Article |
| id | doaj.art-88c03f3c4e1949cf8f304df49dd665f1 |
| institution | Directory Open Access Journal |
| issn | 1663-3563 |
| language | English |
| last_indexed | 2024-12-11T15:01:10Z |
| publishDate | 2015-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Synaptic Neuroscience |
| spelling | doaj.art-88c03f3c4e1949cf8f304df49dd665f12022-12-22T01:01:09ZengFrontiers Media S.A.Frontiers in Synaptic Neuroscience1663-35632015-02-01710.3389/fnsyn.2015.00001125561Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones.Celine eBidoret0Celine eBidoret1Guy eBouvier2Annick eAyon3German eSzapiro4Mariano eCasado5Ecole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris, F-75005 FranceLaboratoire de Neurophysiologie et Nouvelles Microscopies U1128 INSERM, Université Paris Descartes, 45 rue des Saints Pères, 75006 Paris, France.CountryEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris, F-75005 FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris, F-75005 FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris, F-75005 FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and Inserm U1024, and CNRS UMR 8197, Paris, F-75005 FranceN-methyl-D-aspartate receptors (NMDARs) in cerebellar molecular layer interneurones (MLIs) are expressed and activated in unusual ways: at parallel fibre synapses they are only recruited by repetitive stimuli, suggesting an extrasynaptic location, whereas their activation by climbing fibre is purely mediated by spillover. NMDARs are thought to play an important role in plasticity at different levels of the cerebellar circuitry. Evaluation of the location, functional properties and physiological roles of NMDARs will be facilitated by knowledge of the NMDAR isoforms recruited. Here we show that MLI-NMDARs activated by both parallel fibre and climbing fibre inputs have similar kinetics and contain GluN2B but not GluN2A subunits. On the other hand, no evidence was found of functional NMDARs in the axons of MLIs. At the parallel fibre - Purkinje cell (PF-PC) synapse, the activation of GluN2A-containing NMDARs has been shown to be necessary for the induction of long-term depression. Our results therefore provide a clear distinction between the NMDARs located on MLIs and those involved in plasticity at PF-PC synapses.http://journal.frontiersin.org/Journal/10.3389/fnsyn.2015.00001/fullcerebellum1NMDA receptors2interneuron3plasticity4parallel fibers5climbing fibers6. |
| spellingShingle | Celine eBidoret Celine eBidoret Guy eBouvier Annick eAyon German eSzapiro Mariano eCasado Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. Frontiers in Synaptic Neuroscience cerebellum1 NMDA receptors2 interneuron3 plasticity4 parallel fibers5 climbing fibers6. |
| title | Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. |
| title_full | Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. |
| title_fullStr | Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. |
| title_full_unstemmed | Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. |
| title_short | Properties and molecular identity of NMDA receptors at synaptic and non-synaptic inputs in cerebellar molecular layer interneurones. |
| title_sort | properties and molecular identity of nmda receptors at synaptic and non synaptic inputs in cerebellar molecular layer interneurones |
| topic | cerebellum1 NMDA receptors2 interneuron3 plasticity4 parallel fibers5 climbing fibers6. |
| url | http://journal.frontiersin.org/Journal/10.3389/fnsyn.2015.00001/full |
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