Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity
Actinomycetes are the one of the components of the rhizospheric microbial population and useful for producing secondary metabolites such as lytic enzymes, antibiotics, and antifungal. The aim of the study was to isolate the actinomycetes from maize rhizosphere collected from Kupang, East Nusa Tengga...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2018-06-01
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| Series: | Indonesian Journal of Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/33064 |
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| author | Umi Fatmawati Yulin Lestari Anja Meryandini Abdjad Asih Nawangsih Aris Tri Wahyudi |
| author_facet | Umi Fatmawati Yulin Lestari Anja Meryandini Abdjad Asih Nawangsih Aris Tri Wahyudi |
| author_sort | Umi Fatmawati |
| collection | DOAJ |
| description | Actinomycetes are the one of the components of the rhizospheric microbial population and useful for producing secondary metabolites such as lytic enzymes, antibiotics, and antifungal. The aim of the study was to isolate the actinomycetes from maize rhizosphere collected from Kupang, East Nusa Tenggara. The screening was focused on the actinomycetes that showed the ability to produce antibacterial, antifungal, and extracellular enzymes such as amylase, cellulase, and protease. The actinomycetes were isolated using Humic-Acid Vitamin B (HV) agar media. The antagonistic assay was tested against Escherichia coli, Staphylococcus aureus, Sclerotium rolfsii and Fusarium oxysporum. Isolate JKP-8 was an isolate that showed the highest activity in inhibiting the growth of E. coli and S. aureus bacteria. Isolate JKP-5 showed the highest activity in inhibiting the growth of F.oxysporum. There were no actinomycetes isolates that showed an ability to inhibit the growth of S. rolfsii fungus based on dual culture assay. JKP-3 and JKP-4 isolates exhibited the highest ability to hydrolyze amylum, while JKP-5 and JKP-8 isolates exhibited the highest ability to hydrolyze CMC. The results of the amplification of 16S rRNA gene in selected potential isolates JKP 5 and JKP 8 indicated that both isolates belong to the genus Streptomyces. |
| first_indexed | 2024-12-19T07:38:31Z |
| format | Article |
| id | doaj.art-88c27f2c349c493bb235e6e505723870 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-19T07:38:31Z |
| publishDate | 2018-06-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-88c27f2c349c493bb235e6e5057238702022-12-21T20:30:32ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412018-06-01231404710.22146/ijbiotech.3306420360Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activityUmi Fatmawati0Yulin Lestari1Anja Meryandini2Abdjad Asih Nawangsih3Aris Tri Wahyudi4Graduate School of Bogor Agricultural University, Microbiology Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, BogorDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, IndonesiaDepartment of Plant Protection, Faculty of Agricultural Technology, Bogor Agricultural University, IndonesiaDepartment of Plant Protection, Faculty of Agricultural Technology, Bogor Agricultural University, IndonesiaActinomycetes are the one of the components of the rhizospheric microbial population and useful for producing secondary metabolites such as lytic enzymes, antibiotics, and antifungal. The aim of the study was to isolate the actinomycetes from maize rhizosphere collected from Kupang, East Nusa Tenggara. The screening was focused on the actinomycetes that showed the ability to produce antibacterial, antifungal, and extracellular enzymes such as amylase, cellulase, and protease. The actinomycetes were isolated using Humic-Acid Vitamin B (HV) agar media. The antagonistic assay was tested against Escherichia coli, Staphylococcus aureus, Sclerotium rolfsii and Fusarium oxysporum. Isolate JKP-8 was an isolate that showed the highest activity in inhibiting the growth of E. coli and S. aureus bacteria. Isolate JKP-5 showed the highest activity in inhibiting the growth of F.oxysporum. There were no actinomycetes isolates that showed an ability to inhibit the growth of S. rolfsii fungus based on dual culture assay. JKP-3 and JKP-4 isolates exhibited the highest ability to hydrolyze amylum, while JKP-5 and JKP-8 isolates exhibited the highest ability to hydrolyze CMC. The results of the amplification of 16S rRNA gene in selected potential isolates JKP 5 and JKP 8 indicated that both isolates belong to the genus Streptomyces.https://jurnal.ugm.ac.id/ijbiotech/article/view/33064actinomycetesantibacterialantifungalhydrolytic enzymerhizosphere |
| spellingShingle | Umi Fatmawati Yulin Lestari Anja Meryandini Abdjad Asih Nawangsih Aris Tri Wahyudi Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity Indonesian Journal of Biotechnology actinomycetes antibacterial antifungal hydrolytic enzyme rhizosphere |
| title | Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity |
| title_full | Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity |
| title_fullStr | Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity |
| title_full_unstemmed | Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity |
| title_short | Isolation of actinomycetes from maize rhizosphere from Kupang, East Nusa Tenggara Province, and evaluation of their antibacterial, antifungal, and extracellular enzyme activity |
| title_sort | isolation of actinomycetes from maize rhizosphere from kupang east nusa tenggara province and evaluation of their antibacterial antifungal and extracellular enzyme activity |
| topic | actinomycetes antibacterial antifungal hydrolytic enzyme rhizosphere |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/33064 |
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