Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study
BACKGROUND: The need for an anti-human globulin (AHG) cross-match (XM) when the antibody screen (ABS) is negative is debatable and a matter of policy. AIM: (1) To compare the outcomes of type and screen (T and S) method versus the AHG-XM in terms of posttransfusion alloimmunization and hemolytic rea...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2022;volume=16;issue=1;spage=1;epage=6;aulast=Pokhrel |
| _version_ | 1818503504007790592 |
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| author | Anupa Pokhrel Ashish Jain Neelam Marwaha Rana Sandip Singh |
| author_facet | Anupa Pokhrel Ashish Jain Neelam Marwaha Rana Sandip Singh |
| author_sort | Anupa Pokhrel |
| collection | DOAJ |
| description | BACKGROUND: The need for an anti-human globulin (AHG) cross-match (XM) when the antibody screen (ABS) is negative is debatable and a matter of policy.
AIM: (1) To compare the outcomes of type and screen (T and S) method versus the AHG-XM in terms of posttransfusion alloimmunization and hemolytic reactions. (2) Calculation of XM transfusion ratio in both groups.
MATERIALS AND METHODS: The study included 200 patients undergoing elective cardiovascular surgery. Group I patients (n = 100) were issued packed red blood cell units after ABO and RhD typing, an ABS followed by an immediate spin XM (T and S protocol), while Group II (n = 100) patients by an AHG-XM. In Group II patients, if incompatibility was found, then an ABS and identification were performed. A posttransfusion ABS and a direct antiglobulin test (DAT) was done on the 4th day. The XM, ABS (3-cell panel) and DAT were done using the gel technique (Bio-Rad, Switzerland). Thus, the outcomes of T and S method versus the AHG-XM in terms of posttransfusion alloimmunization and hemolytic reactions was measured. The XM transfusion ratio was also calculated in both groups.
RESULTS: In each of Groups I and II, 99 patients (99%) were transfused. There was no significant difference between the two groups based on previous transfusion (P = 0.621) or combined history of transfusion and pregnancy (P = 1). In Group I, all the patients were negative for ABS. In Group II, an AHG-XM was incompatible for 1 patient (1%) due to anti-c and anti-E alloantibodies and had a history of pregnancy as well as transfusion. In both the groups, none of the patients had any adverse transfusion reaction and the posttransfusion ABS and DAT were negative.
CONCLUSION: ABS is a better tool than AHG-XM in detecting alloantibodies in patients having the previous history of transfusion and/or pregnancy. |
| first_indexed | 2024-12-10T21:24:51Z |
| format | Article |
| id | doaj.art-88c29337f4fe4e3583a10df418b080e0 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-12-10T21:24:51Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-88c29337f4fe4e3583a10df418b080e02022-12-22T01:33:01ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652022-01-011611610.4103/ajts.AJTS_21_19Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative studyAnupa PokhrelAshish JainNeelam MarwahaRana Sandip SinghBACKGROUND: The need for an anti-human globulin (AHG) cross-match (XM) when the antibody screen (ABS) is negative is debatable and a matter of policy. AIM: (1) To compare the outcomes of type and screen (T and S) method versus the AHG-XM in terms of posttransfusion alloimmunization and hemolytic reactions. (2) Calculation of XM transfusion ratio in both groups. MATERIALS AND METHODS: The study included 200 patients undergoing elective cardiovascular surgery. Group I patients (n = 100) were issued packed red blood cell units after ABO and RhD typing, an ABS followed by an immediate spin XM (T and S protocol), while Group II (n = 100) patients by an AHG-XM. In Group II patients, if incompatibility was found, then an ABS and identification were performed. A posttransfusion ABS and a direct antiglobulin test (DAT) was done on the 4th day. The XM, ABS (3-cell panel) and DAT were done using the gel technique (Bio-Rad, Switzerland). Thus, the outcomes of T and S method versus the AHG-XM in terms of posttransfusion alloimmunization and hemolytic reactions was measured. The XM transfusion ratio was also calculated in both groups. RESULTS: In each of Groups I and II, 99 patients (99%) were transfused. There was no significant difference between the two groups based on previous transfusion (P = 0.621) or combined history of transfusion and pregnancy (P = 1). In Group I, all the patients were negative for ABS. In Group II, an AHG-XM was incompatible for 1 patient (1%) due to anti-c and anti-E alloantibodies and had a history of pregnancy as well as transfusion. In both the groups, none of the patients had any adverse transfusion reaction and the posttransfusion ABS and DAT were negative. CONCLUSION: ABS is a better tool than AHG-XM in detecting alloantibodies in patients having the previous history of transfusion and/or pregnancy.http://www.ajts.org/article.asp?issn=0973-6247;year=2022;volume=16;issue=1;spage=1;epage=6;aulast=Pokhrelantibody screencross-matchtype and screen |
| spellingShingle | Anupa Pokhrel Ashish Jain Neelam Marwaha Rana Sandip Singh Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study Asian Journal of Transfusion Science antibody screen cross-match type and screen |
| title | Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study |
| title_full | Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study |
| title_fullStr | Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study |
| title_full_unstemmed | Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study |
| title_short | Outcome of type and screen versus crossmatch in cardiovascular surgery patients: A comparative study |
| title_sort | outcome of type and screen versus crossmatch in cardiovascular surgery patients a comparative study |
| topic | antibody screen cross-match type and screen |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2022;volume=16;issue=1;spage=1;epage=6;aulast=Pokhrel |
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