A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons
Summary: Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microsco...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301763 |
| _version_ | 1819077437531619328 |
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| author | Alessia Di Nardo Sara Vasquez Mustafa Sahin |
| author_facet | Alessia Di Nardo Sara Vasquez Mustafa Sahin |
| author_sort | Alessia Di Nardo |
| collection | DOAJ |
| description | Summary: Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microscopic protrusions are crucial given the role of cilia in the brain. We developed a cell-based assay to monitor cilia in rat hippocampal neurons using lentiviral-mediated shRNA-based gene silencing. This optimized platform can be used for high-throughput cilia imaging, disease modeling, and drug screening.For complete details on the use and execution of this protocol, please refer to Di Nardo et al. (2020). |
| first_indexed | 2024-12-21T18:57:11Z |
| format | Article |
| id | doaj.art-88c69fa36ccf4419b9943236f69196ba |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T18:57:11Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-88c69fa36ccf4419b9943236f69196ba2022-12-21T18:53:36ZengElsevierSTAR Protocols2666-16672020-12-0113100189A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal NeuronsAlessia Di Nardo0Sara Vasquez1Mustafa Sahin2F.M. Kirby Neurobiology Center, Translational Neuroscience Center, Department of Neurology, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USAF.M. Kirby Neurobiology Center, Translational Neuroscience Center, Department of Neurology, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USAF.M. Kirby Neurobiology Center, Translational Neuroscience Center, Department of Neurology, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microscopic protrusions are crucial given the role of cilia in the brain. We developed a cell-based assay to monitor cilia in rat hippocampal neurons using lentiviral-mediated shRNA-based gene silencing. This optimized platform can be used for high-throughput cilia imaging, disease modeling, and drug screening.For complete details on the use and execution of this protocol, please refer to Di Nardo et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301763Cell BiologyCell-based AssaysMicroscopyNeuroscience |
| spellingShingle | Alessia Di Nardo Sara Vasquez Mustafa Sahin A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons STAR Protocols Cell Biology Cell-based Assays Microscopy Neuroscience |
| title | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
| title_full | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
| title_fullStr | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
| title_full_unstemmed | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
| title_short | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
| title_sort | cell based assay optimized for high content cilia imaging with primary rat hippocampal neurons |
| topic | Cell Biology Cell-based Assays Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301763 |
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