Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
Abstract Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a m...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-09-01
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| Series: | Horticulture Research |
| Online Access: | https://doi.org/10.1038/s41438-019-0190-y |
| _version_ | 1818117535662342144 |
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| author | Kendra A. McClure YuiHui Gong Jun Song Melinda Vinqvist-Tymchuk Leslie Campbell Palmer Lihua Fan Karen Burgher-MacLellan ZhaoQi Zhang Jean-Marc Celton Charles F. Forney Zoë Migicovsky Sean Myles |
| author_facet | Kendra A. McClure YuiHui Gong Jun Song Melinda Vinqvist-Tymchuk Leslie Campbell Palmer Lihua Fan Karen Burgher-MacLellan ZhaoQi Zhang Jean-Marc Celton Charles F. Forney Zoë Migicovsky Sean Myles |
| author_sort | Kendra A. McClure |
| collection | DOAJ |
| description | Abstract Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a major role because polyphenol concentration varies dramatically among apple cultivars. Here we investigated the genetic architecture of apple polyphenols by combining high performance liquid chromatography (HPLC) data with ~100,000 single nucleotide polymorphisms (SNPs) from two diverse apple populations. We found that polyphenols can vary in concentration by up to two orders of magnitude across cultivars, and that this dramatic variation was often predictable using genetic markers and frequently controlled by a small number of large effect genetic loci. Using GWAS, we identified candidate genes for the production of quercitrin, epicatechin, catechin, chlorogenic acid, 4-O-caffeoylquinic acid and procyanidins B1, B2, and C1. Our observation that a relatively simple genetic architecture underlies the dramatic variation of key polyphenols in apples suggests that breeders may be able to improve the nutritional value of apples through marker-assisted breeding or gene editing. |
| first_indexed | 2024-12-11T04:39:57Z |
| format | Article |
| id | doaj.art-88c735000ecb41398a52c49bddf8b0ab |
| institution | Directory Open Access Journal |
| issn | 2052-7276 |
| language | English |
| last_indexed | 2024-12-11T04:39:57Z |
| publishDate | 2019-09-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Horticulture Research |
| spelling | doaj.art-88c735000ecb41398a52c49bddf8b0ab2022-12-22T01:20:39ZengOxford University PressHorticulture Research2052-72762019-09-016111210.1038/s41438-019-0190-yGenome-wide association studies in apple reveal loci of large effect controlling apple polyphenolsKendra A. McClure0YuiHui Gong1Jun Song2Melinda Vinqvist-Tymchuk3Leslie Campbell Palmer4Lihua Fan5Karen Burgher-MacLellan6ZhaoQi Zhang7Jean-Marc Celton8Charles F. Forney9Zoë Migicovsky10Sean Myles11Department of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie UniversityCollege of Horticulture, South China Agriculture UniversityAgriculture and Agri-Food Canada, Kentville Research and Development CentreAgriculture and Agri-Food Canada, Kentville Research and Development CentreAgriculture and Agri-Food Canada, Kentville Research and Development CentreAgriculture and Agri-Food Canada, Kentville Research and Development CentreAgriculture and Agri-Food Canada, Kentville Research and Development CentreCollege of Horticulture, South China Agriculture UniversityIRHS, Agrocampus-Ouest, INRA, Université d’Angers, SFR 4207 QuaSaVAgriculture and Agri-Food Canada, Kentville Research and Development CentreDepartment of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie UniversityDepartment of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie UniversityAbstract Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing, primarily as dietary antioxidants. Although numerous pre- and post-harvest factors can affect the composition of polyphenols in apples, genetics is presumed to play a major role because polyphenol concentration varies dramatically among apple cultivars. Here we investigated the genetic architecture of apple polyphenols by combining high performance liquid chromatography (HPLC) data with ~100,000 single nucleotide polymorphisms (SNPs) from two diverse apple populations. We found that polyphenols can vary in concentration by up to two orders of magnitude across cultivars, and that this dramatic variation was often predictable using genetic markers and frequently controlled by a small number of large effect genetic loci. Using GWAS, we identified candidate genes for the production of quercitrin, epicatechin, catechin, chlorogenic acid, 4-O-caffeoylquinic acid and procyanidins B1, B2, and C1. Our observation that a relatively simple genetic architecture underlies the dramatic variation of key polyphenols in apples suggests that breeders may be able to improve the nutritional value of apples through marker-assisted breeding or gene editing.https://doi.org/10.1038/s41438-019-0190-y |
| spellingShingle | Kendra A. McClure YuiHui Gong Jun Song Melinda Vinqvist-Tymchuk Leslie Campbell Palmer Lihua Fan Karen Burgher-MacLellan ZhaoQi Zhang Jean-Marc Celton Charles F. Forney Zoë Migicovsky Sean Myles Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols Horticulture Research |
| title | Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| title_full | Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| title_fullStr | Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| title_full_unstemmed | Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| title_short | Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| title_sort | genome wide association studies in apple reveal loci of large effect controlling apple polyphenols |
| url | https://doi.org/10.1038/s41438-019-0190-y |
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