Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging
Nanoprobes that offer both fluorescence imaging (FI) and magnetic resonance imaging (MRI) can provide supplementary information and hold synergistic advantages. However, synthesis of such dual-modality imaging probes that simultaneously exhibit tunability of functional groups, high stability, great...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-01-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22002031 |
| _version_ | 1798000720703651840 |
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| author | Xueyang Xiao Hao Cai Qiaorong Huang Bing Wang Xiaoming Wang Qiang Luo Yinggang Li Hu Zhang Qiyong Gong Xuelei Ma Zhongwei Gu Kui Luo |
| author_facet | Xueyang Xiao Hao Cai Qiaorong Huang Bing Wang Xiaoming Wang Qiang Luo Yinggang Li Hu Zhang Qiyong Gong Xuelei Ma Zhongwei Gu Kui Luo |
| author_sort | Xueyang Xiao |
| collection | DOAJ |
| description | Nanoprobes that offer both fluorescence imaging (FI) and magnetic resonance imaging (MRI) can provide supplementary information and hold synergistic advantages. However, synthesis of such dual-modality imaging probes that simultaneously exhibit tunability of functional groups, high stability, great biocompatibility and desired dual-modality imaging results remains challenging. In this study, we used an amphiphilic block polymer from (ethylene glycol) methyl ether methacrylate (OEGMA) and N-(2-hydroxypropyl) methacrylamide (HPMA) derivatives as a carrier to conjugate a MR contrast agent, Gd-DOTA, and a two-photon fluorophore with an aggregation-induced emission (AIE) effect, TPBP, to construct a MR/two-photon fluorescence dual-modality contrast agent, Gd-DOTA-TPBP. Incorporation of gadolinium in the hydrophilic chain segment of the OEGMA-based carrier resulted in a high r1 value for Gd-DOTA-TPBP, revealing a great MR imaging resolution. The contrast agent specifically accumulated in the tumor region, allowing a long enhancement duration for vascular and tumor contrast-enhanced MR imaging. Meanwhile, coupling TPBP with AIE properties to the hydrophobic chain segment of the carrier not only improved its water solubility and reduced its cytotoxicity, but also significantly enhanced its imaging performance in an aqueous phase. Gd-DOTA-TPBP was also demonstrated to act as an excellent fluorescence probe for two-photon-excited bioimaging with higher resolution and greater sensitivity than MRI. Since high-resolution, complementary MRI/FI dual-modal images were acquired at both cellular and tissue levels in tumor-bearing mice after application of Gd-DOTA-TPBP, it has great potential in the early phase of disease diagnosis. |
| first_indexed | 2024-04-11T11:24:52Z |
| format | Article |
| id | doaj.art-88c9c0d142614f0fabe97e3092e22091 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-11T11:24:52Z |
| publishDate | 2023-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-88c9c0d142614f0fabe97e3092e220912022-12-22T04:26:17ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-01-0119538549Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imagingXueyang Xiao0Hao Cai1Qiaorong Huang2Bing Wang3Xiaoming Wang4Qiang Luo5Yinggang Li6Hu Zhang7Qiyong Gong8Xuelei Ma9Zhongwei Gu10Kui Luo11Department of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaAmgen Bioprocessing Centre, Keck Graduate Institute, CA, 91711, USADepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Functional and Molecular Imaging Key Laboratory of Sichuan Province, Research Unit of Psychoradiology, Chinese Academy of Medical Sciences, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Corresponding author.Department of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, ChinaDepartment of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China; Functional and Molecular Imaging Key Laboratory of Sichuan Province, Research Unit of Psychoradiology, Chinese Academy of Medical Sciences, Chengdu, 610041, China; Corresponding author. Department of Radiology, Department of Biotherapy, Huaxi MR Research Center (HMRRC), Laboratory of Stem Cell Biology, Cancer Center, Department of Ultrasound, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.Nanoprobes that offer both fluorescence imaging (FI) and magnetic resonance imaging (MRI) can provide supplementary information and hold synergistic advantages. However, synthesis of such dual-modality imaging probes that simultaneously exhibit tunability of functional groups, high stability, great biocompatibility and desired dual-modality imaging results remains challenging. In this study, we used an amphiphilic block polymer from (ethylene glycol) methyl ether methacrylate (OEGMA) and N-(2-hydroxypropyl) methacrylamide (HPMA) derivatives as a carrier to conjugate a MR contrast agent, Gd-DOTA, and a two-photon fluorophore with an aggregation-induced emission (AIE) effect, TPBP, to construct a MR/two-photon fluorescence dual-modality contrast agent, Gd-DOTA-TPBP. Incorporation of gadolinium in the hydrophilic chain segment of the OEGMA-based carrier resulted in a high r1 value for Gd-DOTA-TPBP, revealing a great MR imaging resolution. The contrast agent specifically accumulated in the tumor region, allowing a long enhancement duration for vascular and tumor contrast-enhanced MR imaging. Meanwhile, coupling TPBP with AIE properties to the hydrophobic chain segment of the carrier not only improved its water solubility and reduced its cytotoxicity, but also significantly enhanced its imaging performance in an aqueous phase. Gd-DOTA-TPBP was also demonstrated to act as an excellent fluorescence probe for two-photon-excited bioimaging with higher resolution and greater sensitivity than MRI. Since high-resolution, complementary MRI/FI dual-modal images were acquired at both cellular and tissue levels in tumor-bearing mice after application of Gd-DOTA-TPBP, it has great potential in the early phase of disease diagnosis.http://www.sciencedirect.com/science/article/pii/S2452199X22002031RAFT polymerizationAmphiphilic block polymersMagnetic resonance/fluorescence dual-modal imagingTumor/vascular imagingTwo-photon AIE fluorescent contrast agent |
| spellingShingle | Xueyang Xiao Hao Cai Qiaorong Huang Bing Wang Xiaoming Wang Qiang Luo Yinggang Li Hu Zhang Qiyong Gong Xuelei Ma Zhongwei Gu Kui Luo Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging Bioactive Materials RAFT polymerization Amphiphilic block polymers Magnetic resonance/fluorescence dual-modal imaging Tumor/vascular imaging Two-photon AIE fluorescent contrast agent |
| title | Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging |
| title_full | Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging |
| title_fullStr | Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging |
| title_full_unstemmed | Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging |
| title_short | Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging |
| title_sort | polymeric dual modal imaging nanoprobe with two photon aggregation induced emission for fluorescence imaging and gadolinium chelation for magnetic resonance imaging |
| topic | RAFT polymerization Amphiphilic block polymers Magnetic resonance/fluorescence dual-modal imaging Tumor/vascular imaging Two-photon AIE fluorescent contrast agent |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22002031 |
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