Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract
This study evaluated the biosafety, phytochemicals, antioxidant activity, acute toxicological profile and antidiarrhoeal property of n-hexane extract of Afzelia africana seeds. Results from the phytochemical profiling (using gas chromatography - mass spectrometry technique) elicited the presence of...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2022-04-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.ajol.info/index.php/jasem/article/view/226853 |
| _version_ | 1797228242491408384 |
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| author | G. I. Ndukwe J. O. Okhiku F. G. Obomanu G. O. Benjamin |
| author_facet | G. I. Ndukwe J. O. Okhiku F. G. Obomanu G. O. Benjamin |
| author_sort | G. I. Ndukwe |
| collection | DOAJ |
| description |
This study evaluated the biosafety, phytochemicals, antioxidant activity, acute toxicological profile and antidiarrhoeal property of n-hexane extract of Afzelia africana seeds. Results from the phytochemical profiling (using gas chromatography - mass spectrometry technique) elicited the presence of hexadecanoic acid (34.97 %), 9, 12, 15-octadecatrien-1-ol (16.27%) and oleic acid (6.71%) as major compounds associated with antioxidant and antidiarrhoeal effects. The study revealed that A. africana seed possesses antioxidant potentials across four models at 100 ?g/ml (39.6%, 59.6%, 48.11% and 0.765 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, ferrous ion chelating, hydrogen peroxide scavenging, and reducing power assays respectively). No adverse effect or lethality was recorded at ≤5000 mg of extract per kg body weight of tested animals after 14 days observation. In castor oil induced diarrhoea, a significant (p<0.05) increase in delayed onset of stooling and percentage inhibition (up to 92.4%) with decrease in defecation frequency were observed across the treated groups when compared with the control. Similarly, the extract showed a significant (p<0.05) dose dependent decrease in gastrointestinal motility with increase in percentage inhibition (up to 91.9%) in charcoal meal test. In conclusion, Afzelia africana seed antidiarrhoeal property has been validated and its folklore benefit affirmed by this study.
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| first_indexed | 2024-04-24T14:53:35Z |
| format | Article |
| id | doaj.art-88cc37be8ebe4a01b46b0b8e43452b96 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:53:35Z |
| publishDate | 2022-04-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-88cc37be8ebe4a01b46b0b8e43452b962024-04-02T19:47:14ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992022-04-0126410.4314/jasem.v26i4.1Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane ExtractG. I. NdukweJ. O. OkhikuF. G. ObomanuG. O. Benjamin This study evaluated the biosafety, phytochemicals, antioxidant activity, acute toxicological profile and antidiarrhoeal property of n-hexane extract of Afzelia africana seeds. Results from the phytochemical profiling (using gas chromatography - mass spectrometry technique) elicited the presence of hexadecanoic acid (34.97 %), 9, 12, 15-octadecatrien-1-ol (16.27%) and oleic acid (6.71%) as major compounds associated with antioxidant and antidiarrhoeal effects. The study revealed that A. africana seed possesses antioxidant potentials across four models at 100 ?g/ml (39.6%, 59.6%, 48.11% and 0.765 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, ferrous ion chelating, hydrogen peroxide scavenging, and reducing power assays respectively). No adverse effect or lethality was recorded at ≤5000 mg of extract per kg body weight of tested animals after 14 days observation. In castor oil induced diarrhoea, a significant (p<0.05) increase in delayed onset of stooling and percentage inhibition (up to 92.4%) with decrease in defecation frequency were observed across the treated groups when compared with the control. Similarly, the extract showed a significant (p<0.05) dose dependent decrease in gastrointestinal motility with increase in percentage inhibition (up to 91.9%) in charcoal meal test. In conclusion, Afzelia africana seed antidiarrhoeal property has been validated and its folklore benefit affirmed by this study. https://www.ajol.info/index.php/jasem/article/view/226853Biosafety;diarrhoea;Afzelia africana;antioxidant;phytochemical;antidiarrhoeal |
| spellingShingle | G. I. Ndukwe J. O. Okhiku F. G. Obomanu G. O. Benjamin Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract Journal of Applied Sciences and Environmental Management Biosafety; diarrhoea; Afzelia africana; antioxidant; phytochemical; antidiarrhoeal |
| title | Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract |
| title_full | Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract |
| title_fullStr | Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract |
| title_full_unstemmed | Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract |
| title_short | Biosafety, Antioxidant and Antidiarrhoeal Potentials of Afzelia africana Seed n-Hexane Extract |
| title_sort | biosafety antioxidant and antidiarrhoeal potentials of afzelia africana seed n hexane extract |
| topic | Biosafety; diarrhoea; Afzelia africana; antioxidant; phytochemical; antidiarrhoeal |
| url | https://www.ajol.info/index.php/jasem/article/view/226853 |
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