Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda
Horseshoe crabs are a group of marine chelicerates that contain only four extant species, some of which are endangered. Their hemolymph has been widely used in medical applications for endotoxin detection. Nevertheless, there is limited information on the profiles of their hemolymph proteins and the...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2020.579706/full |
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| author | Zhe Qu Zhe Qu Thomas C. N. Leung Wenyan Nong Wenyan Nong Ho Yin Yip Ho Yin Yip Ivy H. T. Lee Ivy H. T. Lee Siu Gin Cheung Ngai Sai Ming Wai Lok So Wai Lok So William G. Bendena Stephen S. Tobe Jerome H. L. Hui Jerome H. L. Hui |
| author_facet | Zhe Qu Zhe Qu Thomas C. N. Leung Wenyan Nong Wenyan Nong Ho Yin Yip Ho Yin Yip Ivy H. T. Lee Ivy H. T. Lee Siu Gin Cheung Ngai Sai Ming Wai Lok So Wai Lok So William G. Bendena Stephen S. Tobe Jerome H. L. Hui Jerome H. L. Hui |
| author_sort | Zhe Qu |
| collection | DOAJ |
| description | Horseshoe crabs are a group of marine chelicerates that contain only four extant species, some of which are endangered. Their hemolymph has been widely used in medical applications for endotoxin detection. Nevertheless, there is limited information on the profiles of their hemolymph proteins and their gut microbial diversity. In this study, we performed the first detailed investigation of the hemolymph proteomics and gut microbiota of two Asian horseshoe crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda. Among the identified proteins being cataloged in the juvenile and adult hemolymph, unexpectedly, sesquiterpenoid signaling pathway proteins including Heat shock protein 83 (HSP83), Chd64, and a juvenile hormone binding protein (JHBP) were revealed. This provides evidence for the presence of functional sesquiterpenoid hormonal systems in these marine chelicerates. consumption of certain horseshoe crab species often leads to tetrodotoxin poisoning and the horseshoe crab is thought to possess a tetrodotoxin resistance mechanism. As such, sodium channels were analyzed and found to have critical amino acid residues that are similar to the toxin resistant pufferfish sodium channels. The source of the toxin is unknown so we investigated the gut microbiota, and found that Clostridium and Vibrio were the most dominant bacteria in T. tridentatus and C. rotundicauda, respectively. Together, this study provides a framework for further understanding of sesquiterpenoids and gut microbiota of these marine chelicerates. |
| first_indexed | 2024-12-13T18:10:32Z |
| format | Article |
| id | doaj.art-88cd7182ab774fa38978a1a179ed8e21 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-13T18:10:32Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-88cd7182ab774fa38978a1a179ed8e212022-12-21T23:35:58ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-12-01710.3389/fmars.2020.579706579706Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicaudaZhe Qu0Zhe Qu1Thomas C. N. Leung2Wenyan Nong3Wenyan Nong4Ho Yin Yip5Ho Yin Yip6Ivy H. T. Lee7Ivy H. T. Lee8Siu Gin Cheung9Ngai Sai Ming10Wai Lok So11Wai Lok So12William G. Bendena13Stephen S. Tobe14Jerome H. L. Hui15Jerome H. L. Hui16Simon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaSimon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaSimon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaSimon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Chemistry, City University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaSimon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Biology, Queen’s University, Kingston, ON, CanadaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, CanadaSimon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaHorseshoe crabs are a group of marine chelicerates that contain only four extant species, some of which are endangered. Their hemolymph has been widely used in medical applications for endotoxin detection. Nevertheless, there is limited information on the profiles of their hemolymph proteins and their gut microbial diversity. In this study, we performed the first detailed investigation of the hemolymph proteomics and gut microbiota of two Asian horseshoe crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda. Among the identified proteins being cataloged in the juvenile and adult hemolymph, unexpectedly, sesquiterpenoid signaling pathway proteins including Heat shock protein 83 (HSP83), Chd64, and a juvenile hormone binding protein (JHBP) were revealed. This provides evidence for the presence of functional sesquiterpenoid hormonal systems in these marine chelicerates. consumption of certain horseshoe crab species often leads to tetrodotoxin poisoning and the horseshoe crab is thought to possess a tetrodotoxin resistance mechanism. As such, sodium channels were analyzed and found to have critical amino acid residues that are similar to the toxin resistant pufferfish sodium channels. The source of the toxin is unknown so we investigated the gut microbiota, and found that Clostridium and Vibrio were the most dominant bacteria in T. tridentatus and C. rotundicauda, respectively. Together, this study provides a framework for further understanding of sesquiterpenoids and gut microbiota of these marine chelicerates.https://www.frontiersin.org/articles/10.3389/fmars.2020.579706/fullhemolymphproteomicssesquiterpenoidsgut microbiotahorseshoe crabs |
| spellingShingle | Zhe Qu Zhe Qu Thomas C. N. Leung Wenyan Nong Wenyan Nong Ho Yin Yip Ho Yin Yip Ivy H. T. Lee Ivy H. T. Lee Siu Gin Cheung Ngai Sai Ming Wai Lok So Wai Lok So William G. Bendena Stephen S. Tobe Jerome H. L. Hui Jerome H. L. Hui Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda Frontiers in Marine Science hemolymph proteomics sesquiterpenoids gut microbiota horseshoe crabs |
| title | Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda |
| title_full | Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda |
| title_fullStr | Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda |
| title_full_unstemmed | Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda |
| title_short | Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda |
| title_sort | hemolymph proteomics and gut microbiota of horseshoe crabs tachypleus tridentatus and carcinoscorpius rotundicauda |
| topic | hemolymph proteomics sesquiterpenoids gut microbiota horseshoe crabs |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2020.579706/full |
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