<i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities
The composition of essential oils of <i>Chrysanthemum indicum</i> and <i>C. morifolium</i> were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determi...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/10/1460 |
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| author | Fadia S. Youssef Safaa Y. Eid Elham Alshammari Mohamed L. Ashour Michael Wink Mahmoud Z. El-Readi |
| author_facet | Fadia S. Youssef Safaa Y. Eid Elham Alshammari Mohamed L. Ashour Michael Wink Mahmoud Z. El-Readi |
| author_sort | Fadia S. Youssef |
| collection | DOAJ |
| description | The composition of essential oils of <i>Chrysanthemum indicum</i> and <i>C. morifolium</i> were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay against three common viruses namely, herpes simplex type-1 (HSV-1), hepatitis A (HAV) and vesicular stomatitis virus (VSV). The antimicrobial activity was assessed using agar diffusion and microdilution methods and the minimum inhibitory concentration (MIC) values were determined. In addition, the anti-mycobacterial evaluation was carried out using the Alamar blue assay and the effect against <i>Helicobacter pylori</i> was investigated. The anti-trypanosomal activity was evaluated using the resazurin method. GC investigations revealed that camphor is the major constituent of both oils accounting for 36.69 and 14.56% in the essential oils from <i>C. indicum</i> and <i>C. morifolium</i>, respectively. <i>C. indicum</i> was biologically more active in all experiments; it exhibited a notable antitrypanosomal activity with an IC<sub>50</sub> value equals 45.89 μg/mL and a notable antimicrobial activity versus <i>Streptococcus agalactiae</i> with a MIC value of 62.5 μg/mL. It also inhibited the replication of VSV with an IC<sub>50</sub> value of 3.14 μg/mL. Both oils revealed antioxidant potential with IC<sub>50</sub> values of 2.21 and 2.59 mg/mL for <i>C. indicum</i> and <i>C. morifolium,</i> respectively. This study provides evidence beyond the traditional use of both <i>Chrysanthemum indicum</i> and <i>C. morifolium</i> as anti-infective agents. Thus they could be used as spices in food and can be incorporated in different food products and pharmaceutical preparations as natural preservatives possessing antioxidant potential. |
| first_indexed | 2024-03-10T15:38:40Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:38:40Z |
| publishDate | 2020-10-01 |
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| series | Foods |
| spelling | doaj.art-88ce5240a0ce4003ac8036c6a44facab2023-11-20T17:02:55ZengMDPI AGFoods2304-81582020-10-01910146010.3390/foods9101460<i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant ActivitiesFadia S. Youssef0Safaa Y. Eid1Elham Alshammari2Mohamed L. Ashour3Michael Wink4Mahmoud Z. El-Readi5Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, EgyptDepartment of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, EgyptDepartment of Pharmaceutical Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, GermanyDepartment of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaThe composition of essential oils of <i>Chrysanthemum indicum</i> and <i>C. morifolium</i> were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay against three common viruses namely, herpes simplex type-1 (HSV-1), hepatitis A (HAV) and vesicular stomatitis virus (VSV). The antimicrobial activity was assessed using agar diffusion and microdilution methods and the minimum inhibitory concentration (MIC) values were determined. In addition, the anti-mycobacterial evaluation was carried out using the Alamar blue assay and the effect against <i>Helicobacter pylori</i> was investigated. The anti-trypanosomal activity was evaluated using the resazurin method. GC investigations revealed that camphor is the major constituent of both oils accounting for 36.69 and 14.56% in the essential oils from <i>C. indicum</i> and <i>C. morifolium</i>, respectively. <i>C. indicum</i> was biologically more active in all experiments; it exhibited a notable antitrypanosomal activity with an IC<sub>50</sub> value equals 45.89 μg/mL and a notable antimicrobial activity versus <i>Streptococcus agalactiae</i> with a MIC value of 62.5 μg/mL. It also inhibited the replication of VSV with an IC<sub>50</sub> value of 3.14 μg/mL. Both oils revealed antioxidant potential with IC<sub>50</sub> values of 2.21 and 2.59 mg/mL for <i>C. indicum</i> and <i>C. morifolium,</i> respectively. This study provides evidence beyond the traditional use of both <i>Chrysanthemum indicum</i> and <i>C. morifolium</i> as anti-infective agents. Thus they could be used as spices in food and can be incorporated in different food products and pharmaceutical preparations as natural preservatives possessing antioxidant potential.https://www.mdpi.com/2304-8158/9/10/1460Asteraceaeantimicrobialantioxidant<i>Chrysanthemum</i>essential oilpreservative |
| spellingShingle | Fadia S. Youssef Safaa Y. Eid Elham Alshammari Mohamed L. Ashour Michael Wink Mahmoud Z. El-Readi <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities Foods Asteraceae antimicrobial antioxidant <i>Chrysanthemum</i> essential oil preservative |
| title | <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities |
| title_full | <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities |
| title_fullStr | <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities |
| title_full_unstemmed | <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities |
| title_short | <i>Chrysanthemum indicum</i> and <i>Chrysanthemum morifolium</i>: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities |
| title_sort | i chrysanthemum indicum i and i chrysanthemum morifolium i chemical composition of their essential oils and their potential use as natural preservatives with antimicrobial and antioxidant activities |
| topic | Asteraceae antimicrobial antioxidant <i>Chrysanthemum</i> essential oil preservative |
| url | https://www.mdpi.com/2304-8158/9/10/1460 |
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