LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis
Tian-jun Chen,1,* Fei Gao,1,2,* Tian Yang,1 Hong Li,1 Yang Li,1 Hui Ren,1 Ming-wei Chen1 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, People’s Republic of China; 2Ultrasound Dep...
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Dove Medical Press
2020-06-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/lncrna-hotairm1-inhibits-the-proliferation-and-invasion-of-lung-adenoc-peer-reviewed-article-CMAR |
| _version_ | 1818450404044701696 |
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| author | Chen T Gao F Yang T Li H Li Y Ren H Chen M |
| author_facet | Chen T Gao F Yang T Li H Li Y Ren H Chen M |
| author_sort | Chen T |
| collection | DOAJ |
| description | Tian-jun Chen,1,* Fei Gao,1,2,* Tian Yang,1 Hong Li,1 Yang Li,1 Hui Ren,1 Ming-wei Chen1 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, People’s Republic of China; 2Ultrasound Department, Huashan Central Hospital of Xi’an, Xi’an, Shaanxi 710043, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ming-wei ChenDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, Yanta West Road, Xi’an, Shaanxi, People’s Republic of ChinaTel +86 29-85323888Email wosyishen66@163.comBackground: Lung adenocarcinoma (ADC) is a major form of lung cancer, which is a main cause of global cancer-related death in male and female patients. LncRNAs are implicated in tumor development. However, the functions and mechanisms of the LncRNA HOTAIRM1 in ADC are not known.Materials and Methods: Here, the downregulated HOTAIRM1 in ADC was selected by TCGA analysis. Subsequently, qRT-PCR, CCK-8, EdU, cell apoptosis, cell cycle and cell invasion assays were utilized for evaluating the roles of HOTAIRM1 in ADC. Finally, we explored the mechanism of HOTAIRM1 in ADC.Results: HOTAIRM1 expression was considerably decreased in ADC tissues. The knockdown of HOTAIRM1 promoted the cell cycle, growth, and invasion of ADC. Moreover, HOTAIRM1 competitively bound miR-498 to regulate the expression of WWOX.Conclusion: HOTAIRM1 suppressed the proliferation and invasion of ADC cells via the modulation of miR-498/WWOX axis. This finding suggested that it might be clinically valuable as a biomarker for ADC. Furthermore, the findings suggest LncRNA HOTAIRM1 as a candidate therapeutic target in ADC.Keywords: HOTAIRM1, lung adenocarcinoma, miR-498, WWOX, cell proliferation |
| first_indexed | 2024-12-14T20:50:45Z |
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| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-14T20:50:45Z |
| publishDate | 2020-06-01 |
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| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-88d21f73fa8944cdbf5bd47ba56115f32022-12-21T22:47:50ZengDove Medical PressCancer Management and Research1179-13222020-06-01Volume 124379439054389LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX AxisChen TGao FYang TLi HLi YRen HChen MTian-jun Chen,1,* Fei Gao,1,2,* Tian Yang,1 Hong Li,1 Yang Li,1 Hui Ren,1 Ming-wei Chen1 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, People’s Republic of China; 2Ultrasound Department, Huashan Central Hospital of Xi’an, Xi’an, Shaanxi 710043, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ming-wei ChenDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, Yanta West Road, Xi’an, Shaanxi, People’s Republic of ChinaTel +86 29-85323888Email wosyishen66@163.comBackground: Lung adenocarcinoma (ADC) is a major form of lung cancer, which is a main cause of global cancer-related death in male and female patients. LncRNAs are implicated in tumor development. However, the functions and mechanisms of the LncRNA HOTAIRM1 in ADC are not known.Materials and Methods: Here, the downregulated HOTAIRM1 in ADC was selected by TCGA analysis. Subsequently, qRT-PCR, CCK-8, EdU, cell apoptosis, cell cycle and cell invasion assays were utilized for evaluating the roles of HOTAIRM1 in ADC. Finally, we explored the mechanism of HOTAIRM1 in ADC.Results: HOTAIRM1 expression was considerably decreased in ADC tissues. The knockdown of HOTAIRM1 promoted the cell cycle, growth, and invasion of ADC. Moreover, HOTAIRM1 competitively bound miR-498 to regulate the expression of WWOX.Conclusion: HOTAIRM1 suppressed the proliferation and invasion of ADC cells via the modulation of miR-498/WWOX axis. This finding suggested that it might be clinically valuable as a biomarker for ADC. Furthermore, the findings suggest LncRNA HOTAIRM1 as a candidate therapeutic target in ADC.Keywords: HOTAIRM1, lung adenocarcinoma, miR-498, WWOX, cell proliferationhttps://www.dovepress.com/lncrna-hotairm1-inhibits-the-proliferation-and-invasion-of-lung-adenoc-peer-reviewed-article-CMARhotairm1lung adenocarcinomamir-498wwoxcell proliferation |
| spellingShingle | Chen T Gao F Yang T Li H Li Y Ren H Chen M LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis Cancer Management and Research hotairm1 lung adenocarcinoma mir-498 wwox cell proliferation |
| title | LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis |
| title_full | LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis |
| title_fullStr | LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis |
| title_full_unstemmed | LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis |
| title_short | LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis |
| title_sort | lncrna hotairm1 inhibits the proliferation and invasion of lung adenocarcinoma cells via the mir 498 wwox axis |
| topic | hotairm1 lung adenocarcinoma mir-498 wwox cell proliferation |
| url | https://www.dovepress.com/lncrna-hotairm1-inhibits-the-proliferation-and-invasion-of-lung-adenoc-peer-reviewed-article-CMAR |
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