Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i>
Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to cap...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/2/282 |
| _version_ | 1811274042617888768 |
|---|---|
| author | Adeilso Bispo dos Santos Junior Cinthia I. Tamayose Marcelo J. P. Ferreira Mariana N. Belchor Caroline R. C. Costa Marcos Antonio de Oliveira Marcos Hikari Toyama |
| author_facet | Adeilso Bispo dos Santos Junior Cinthia I. Tamayose Marcelo J. P. Ferreira Mariana N. Belchor Caroline R. C. Costa Marcos Antonio de Oliveira Marcos Hikari Toyama |
| author_sort | Adeilso Bispo dos Santos Junior |
| collection | DOAJ |
| description | Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on <i>Crotalus durissus terrificus</i> sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the <i>Moquiniastrum floribundum</i> leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from <i>Crotalus durissus terrificus</i>. |
| first_indexed | 2024-04-12T23:11:55Z |
| format | Article |
| id | doaj.art-88d3e4de808541749c5a053b6b55a246 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T23:11:55Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-88d3e4de808541749c5a053b6b55a2462022-12-22T03:12:47ZengMDPI AGMolecules1420-30492020-01-0125228210.3390/molecules25020282molecules25020282Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i>Adeilso Bispo dos Santos Junior0Cinthia I. Tamayose1Marcelo J. P. Ferreira2Mariana N. Belchor3Caroline R. C. Costa4Marcos Antonio de Oliveira5Marcos Hikari Toyama6BIOMOLPEP group, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, BrazilDepartamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo CEP 05508-090, BrazilDepartamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo CEP 05508-090, BrazilBIOMOLPEP group, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, BrazilBIOMOLPEP group, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, BrazilLABIMES, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, BrazilBIOMOLPEP group, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, BrazilBioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on <i>Crotalus durissus terrificus</i> sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the <i>Moquiniastrum floribundum</i> leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from <i>Crotalus durissus terrificus</i>.https://www.mdpi.com/1420-3049/25/2/282ultrafiltrationanti-inflammatorynatural compoundsbioaffinity |
| spellingShingle | Adeilso Bispo dos Santos Junior Cinthia I. Tamayose Marcelo J. P. Ferreira Mariana N. Belchor Caroline R. C. Costa Marcos Antonio de Oliveira Marcos Hikari Toyama Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> Molecules ultrafiltration anti-inflammatory natural compounds bioaffinity |
| title | Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> |
| title_full | Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> |
| title_fullStr | Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> |
| title_full_unstemmed | Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> |
| title_short | Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from <i>Crotalus durissus terrificus</i> by Hispidulin from <i>Moquiniastrum floribundum</i> |
| title_sort | bioaffinity fishing procedure using secretory phospholipase a2 for screening for bioactive components modulation of pharmacological effect induced by spla2 from i crotalus durissus terrificus i by hispidulin from i moquiniastrum floribundum i |
| topic | ultrafiltration anti-inflammatory natural compounds bioaffinity |
| url | https://www.mdpi.com/1420-3049/25/2/282 |
| work_keys_str_mv | AT adeilsobispodossantosjunior bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT cinthiaitamayose bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT marcelojpferreira bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT mariananbelchor bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT carolinerccosta bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT marcosantoniodeoliveira bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi AT marcoshikaritoyama bioaffinityfishingprocedureusingsecretoryphospholipasea2forscreeningforbioactivecomponentsmodulationofpharmacologicaleffectinducedbyspla2fromicrotalusdurissusterrificusibyhispidulinfromimoquiniastrumfloribundumi |