Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232
Abstract The food enzyme mannan endo‐1,4‐β‐mannosidase (1,4‐β‐d‐mannan mannanohydrolase; EC 3.2.1.78) is produced with the genetically modified Trichoderma reesei strain RF6232 by AB Enzymes GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme was considered free from...
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | EFSA Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2903/j.efsa.2022.7478 |
| _version_ | 1797999755659313152 |
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| author | EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) Claude Lambré José Manuel Barat Baviera Claudia Bolognesi Pier Sandro Cocconcelli Riccardo Crebelli David Michael Gott Konrad Grob Evgenia Lampi Marcel Mengelers Alicja Mortensen Gilles Rivière Inger‐Lise Steffensen Christina Tlustos Henk Van Loveren Laurence Vernis Holger Zorn Lieve Herman Joaquim Maia Natalia Kovalkovicova Yi Liu Simone Lunardi Giulio diPiazza Rita Ferreira de Sousa Andrew Chesson |
| author_facet | EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) Claude Lambré José Manuel Barat Baviera Claudia Bolognesi Pier Sandro Cocconcelli Riccardo Crebelli David Michael Gott Konrad Grob Evgenia Lampi Marcel Mengelers Alicja Mortensen Gilles Rivière Inger‐Lise Steffensen Christina Tlustos Henk Van Loveren Laurence Vernis Holger Zorn Lieve Herman Joaquim Maia Natalia Kovalkovicova Yi Liu Simone Lunardi Giulio diPiazza Rita Ferreira de Sousa Andrew Chesson |
| author_sort | EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) |
| collection | DOAJ |
| description | Abstract The food enzyme mannan endo‐1,4‐β‐mannosidase (1,4‐β‐d‐mannan mannanohydrolase; EC 3.2.1.78) is produced with the genetically modified Trichoderma reesei strain RF6232 by AB Enzymes GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme was considered free from viable cells of the production organism and recombinant DNA. It is intended to be used in coffee processing, fruit and vegetable processing for juice production and for edible oil production. Since residual amounts of total organic solids (TOS) are removed during refined edible oil production by repeated washing, dietary exposure was calculated only for the remaining two food manufacturing processes. Dietary exposure to the food enzyme–TOS was estimated to be up to 0.09 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not raise a safety concern. The systemic toxicity was assessed by a repeated dose 90‐day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 100 mg TOS/kg bw per day, the lowest dose tested. This results in a margin of exposure above 1,100. A search for similarity of the amino acid sequence of the food enzyme to known allergens was made and one match was found. The Panel considered that, under the intended conditions of use, the risk of allergic sensitisation and elicitation reactions by dietary exposure cannot be excluded, in particular for individuals allergic to avocado, but the likelihood for this to occur is considered to be low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use. |
| first_indexed | 2024-04-11T11:09:47Z |
| format | Article |
| id | doaj.art-88d5b865c7a44116babb17f7a831ceec |
| institution | Directory Open Access Journal |
| issn | 1831-4732 |
| language | English |
| last_indexed | 2024-04-11T11:09:47Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | EFSA Journal |
| spelling | doaj.art-88d5b865c7a44116babb17f7a831ceec2022-12-22T04:27:57ZengWileyEFSA Journal1831-47322022-08-01208n/an/a10.2903/j.efsa.2022.7478Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)Claude LambréJosé Manuel Barat BavieraClaudia BolognesiPier Sandro CocconcelliRiccardo CrebelliDavid Michael GottKonrad GrobEvgenia LampiMarcel MengelersAlicja MortensenGilles RivièreInger‐Lise SteffensenChristina TlustosHenk Van LoverenLaurence VernisHolger ZornLieve HermanJoaquim MaiaNatalia KovalkovicovaYi LiuSimone LunardiGiulio diPiazzaRita Ferreira de SousaAndrew ChessonAbstract The food enzyme mannan endo‐1,4‐β‐mannosidase (1,4‐β‐d‐mannan mannanohydrolase; EC 3.2.1.78) is produced with the genetically modified Trichoderma reesei strain RF6232 by AB Enzymes GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme was considered free from viable cells of the production organism and recombinant DNA. It is intended to be used in coffee processing, fruit and vegetable processing for juice production and for edible oil production. Since residual amounts of total organic solids (TOS) are removed during refined edible oil production by repeated washing, dietary exposure was calculated only for the remaining two food manufacturing processes. Dietary exposure to the food enzyme–TOS was estimated to be up to 0.09 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not raise a safety concern. The systemic toxicity was assessed by a repeated dose 90‐day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 100 mg TOS/kg bw per day, the lowest dose tested. This results in a margin of exposure above 1,100. A search for similarity of the amino acid sequence of the food enzyme to known allergens was made and one match was found. The Panel considered that, under the intended conditions of use, the risk of allergic sensitisation and elicitation reactions by dietary exposure cannot be excluded, in particular for individuals allergic to avocado, but the likelihood for this to occur is considered to be low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use.https://doi.org/10.2903/j.efsa.2022.7478food enzymemannan endo‐1,4‐β‐mannosidase1,4‐β‐d‐mannan mannanohydrolaseβ‐mannanaseEC 3.2.1.78Trichoderma reesei |
| spellingShingle | EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) Claude Lambré José Manuel Barat Baviera Claudia Bolognesi Pier Sandro Cocconcelli Riccardo Crebelli David Michael Gott Konrad Grob Evgenia Lampi Marcel Mengelers Alicja Mortensen Gilles Rivière Inger‐Lise Steffensen Christina Tlustos Henk Van Loveren Laurence Vernis Holger Zorn Lieve Herman Joaquim Maia Natalia Kovalkovicova Yi Liu Simone Lunardi Giulio diPiazza Rita Ferreira de Sousa Andrew Chesson Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 EFSA Journal food enzyme mannan endo‐1,4‐β‐mannosidase 1,4‐β‐d‐mannan mannanohydrolase β‐mannanase EC 3.2.1.78 Trichoderma reesei |
| title | Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 |
| title_full | Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 |
| title_fullStr | Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 |
| title_full_unstemmed | Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 |
| title_short | Safety evaluation of the food enzyme mannan endo‐1,4‐β‐mannosidase from the genetically modified Trichoderma reesei strain RF6232 |
| title_sort | safety evaluation of the food enzyme mannan endo 1 4 β mannosidase from the genetically modified trichoderma reesei strain rf6232 |
| topic | food enzyme mannan endo‐1,4‐β‐mannosidase 1,4‐β‐d‐mannan mannanohydrolase β‐mannanase EC 3.2.1.78 Trichoderma reesei |
| url | https://doi.org/10.2903/j.efsa.2022.7478 |
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