Results in the determination of some Hosta varieties by the method of isoelectric focusing
Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenolo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Debrecen
2001-03-01
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| Series: | International Journal of Horticultural Science |
| Subjects: | |
| Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/256 |
| _version_ | 1828235979177066496 |
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| author | A. Mándy É. Stefanovits-Bányai Cs. Imre Zs. Szafián |
| author_facet | A. Mándy É. Stefanovits-Bányai Cs. Imre Zs. Szafián |
| author_sort | A. Mándy |
| collection | DOAJ |
| description | Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation. |
| first_indexed | 2024-04-12T20:25:13Z |
| format | Article |
| id | doaj.art-88d6d40743e44c228387207e2cd769da |
| institution | Directory Open Access Journal |
| issn | 1585-0404 2676-931X |
| language | English |
| last_indexed | 2024-04-12T20:25:13Z |
| publishDate | 2001-03-01 |
| publisher | University of Debrecen |
| record_format | Article |
| series | International Journal of Horticultural Science |
| spelling | doaj.art-88d6d40743e44c228387207e2cd769da2022-12-22T03:17:54ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2001-03-017110.31421/IJHS/7/1/256Results in the determination of some Hosta varieties by the method of isoelectric focusingA. Mándy0É. Stefanovits-Bányai1Cs. Imre2Zs. Szafián3Univ. of Horticulture and Food Dep. of Floriculture and Dendrology, 1118 Budapest, Villányi út 35-43., HungaryUniv. of Horticulture and Food Dep. of Chemistry and Biochemistry, 1118 Budapest, Villányi út 35-43., HungaryUniv. of Agricultural Science Dep. of Horticulture, Gyöngyös, Mátrai út 2., HungaryPRENOR Nursery, 9707 Szombathely, Béke tér 1., HungaryMass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.https://ojs.lib.unideb.hu/IJHS/article/view/256Hostamicropropagationisoenzyme patternperoxidasespecies variabilityisoelectric focusing |
| spellingShingle | A. Mándy É. Stefanovits-Bányai Cs. Imre Zs. Szafián Results in the determination of some Hosta varieties by the method of isoelectric focusing International Journal of Horticultural Science Hosta micropropagation isoenzyme pattern peroxidase species variability isoelectric focusing |
| title | Results in the determination of some Hosta varieties by the method of isoelectric focusing |
| title_full | Results in the determination of some Hosta varieties by the method of isoelectric focusing |
| title_fullStr | Results in the determination of some Hosta varieties by the method of isoelectric focusing |
| title_full_unstemmed | Results in the determination of some Hosta varieties by the method of isoelectric focusing |
| title_short | Results in the determination of some Hosta varieties by the method of isoelectric focusing |
| title_sort | results in the determination of some hosta varieties by the method of isoelectric focusing |
| topic | Hosta micropropagation isoenzyme pattern peroxidase species variability isoelectric focusing |
| url | https://ojs.lib.unideb.hu/IJHS/article/view/256 |
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