Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean
Soybean (Glycine max) is an important crop in agricultural production where water shortage limits yields in soybean. Root system plays important roles in water-limited environments, but the underlying mechanisms are largely unknown. In our previous study, we produced a RNA-seq dataset generated from...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1186292/full |
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| author | Mitra Mazarei Mitra Mazarei Pratyush Routray Sarbottam Piya C. Neal Stewart C. Neal Stewart Tarek Hewezi |
| author_facet | Mitra Mazarei Mitra Mazarei Pratyush Routray Sarbottam Piya C. Neal Stewart C. Neal Stewart Tarek Hewezi |
| author_sort | Mitra Mazarei |
| collection | DOAJ |
| description | Soybean (Glycine max) is an important crop in agricultural production where water shortage limits yields in soybean. Root system plays important roles in water-limited environments, but the underlying mechanisms are largely unknown. In our previous study, we produced a RNA-seq dataset generated from roots of soybean at three different growth stages (20-, 30-, and 44-day-old plants). In the present study, we performed a transcriptome analysis of the RNA-seq data to select candidate genes with probable association with root growth and development. Candidate genes were functionally examined in soybean by overexpression of individual genes using intact soybean composite plants with transgenic hairy roots. Root growth and biomass in the transgenic composite plants were significantly increased by overexpression of the GmNAC19 and GmGRAB1 transcriptional factors, showing up to 1.8-fold increase in root length and/or 1.7-fold increase in root fresh/dry weight. Furthermore, greenhouse-grown transgenic composite plants had significantly higher seed yield by about 2-fold than control plants. Expression profiling in different developmental stages and tissues showed that GmNAC19 and GmGRAB1 were most highly expressed in roots, displaying a distinct root-preferential expression. Moreover, we found that under water-deficit conditions, overexpression of GmNAC19 enhanced water stress tolerance in transgenic composite plants. Taken together, these results provide further insights into the agricultural potential of these genes for development of soybean cultivars with improved root growth and enhanced tolerance to water-deficit conditions. |
| first_indexed | 2024-03-13T08:23:55Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-13T08:23:55Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-88d6e91ae6714a6a82a266c8f3b9bfa92023-05-31T05:00:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11862921186292Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybeanMitra Mazarei0Mitra Mazarei1Pratyush Routray2Sarbottam Piya3C. Neal Stewart4C. Neal Stewart5Tarek Hewezi6Department of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesCenter for Agricultural Synthetic Biology, University of Tennessee, Knoxville, TN, United StatesDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesCenter for Agricultural Synthetic Biology, University of Tennessee, Knoxville, TN, United StatesDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesSoybean (Glycine max) is an important crop in agricultural production where water shortage limits yields in soybean. Root system plays important roles in water-limited environments, but the underlying mechanisms are largely unknown. In our previous study, we produced a RNA-seq dataset generated from roots of soybean at three different growth stages (20-, 30-, and 44-day-old plants). In the present study, we performed a transcriptome analysis of the RNA-seq data to select candidate genes with probable association with root growth and development. Candidate genes were functionally examined in soybean by overexpression of individual genes using intact soybean composite plants with transgenic hairy roots. Root growth and biomass in the transgenic composite plants were significantly increased by overexpression of the GmNAC19 and GmGRAB1 transcriptional factors, showing up to 1.8-fold increase in root length and/or 1.7-fold increase in root fresh/dry weight. Furthermore, greenhouse-grown transgenic composite plants had significantly higher seed yield by about 2-fold than control plants. Expression profiling in different developmental stages and tissues showed that GmNAC19 and GmGRAB1 were most highly expressed in roots, displaying a distinct root-preferential expression. Moreover, we found that under water-deficit conditions, overexpression of GmNAC19 enhanced water stress tolerance in transgenic composite plants. Taken together, these results provide further insights into the agricultural potential of these genes for development of soybean cultivars with improved root growth and enhanced tolerance to water-deficit conditions.https://www.frontiersin.org/articles/10.3389/fpls.2023.1186292/fullsoybeanGmNAC19GmGRAB1GmTUBBYoverexpressionroot growth |
| spellingShingle | Mitra Mazarei Mitra Mazarei Pratyush Routray Sarbottam Piya C. Neal Stewart C. Neal Stewart Tarek Hewezi Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean Frontiers in Plant Science soybean GmNAC19 GmGRAB1 GmTUBBY overexpression root growth |
| title | Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean |
| title_full | Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean |
| title_fullStr | Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean |
| title_full_unstemmed | Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean |
| title_short | Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean |
| title_sort | overexpression of soybean gmnac19 and gmgrab1 enhances root growth and water deficit stress tolerance in soybean |
| topic | soybean GmNAC19 GmGRAB1 GmTUBBY overexpression root growth |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1186292/full |
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