Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge
Recovering microorganisms from environmental samples is a crucial primary step for understanding microbial communities using molecular ecological approaches. It is often challenging to harvest microorganisms both efficiently and unselectively, guaranteeing a similar microbial composition between ori...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-12-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01891/full |
| _version_ | 1811243198466490368 |
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| author | Xin-Lei Gao Mingfei Shao Yi-Sheng Xu Yi Luo Kai Zhang Feng Ouyang Ji Li |
| author_facet | Xin-Lei Gao Mingfei Shao Yi-Sheng Xu Yi Luo Kai Zhang Feng Ouyang Ji Li |
| author_sort | Xin-Lei Gao |
| collection | DOAJ |
| description | Recovering microorganisms from environmental samples is a crucial primary step for understanding microbial communities using molecular ecological approaches. It is often challenging to harvest microorganisms both efficiently and unselectively, guaranteeing a similar microbial composition between original and separated biomasses. A magnetic nanoparticles (MNPs) based method was developed to effectively separate microbial biomass from glass fiber pulp entrapped bacteria. Buffering pH and nanoparticle silica encapsulation significantly affected both biomass recovery and microbial selectivity. Under optimized conditions (using citric acid coated Fe3O4, buffering pH=2.2), the method was applied in the pretreatment of TSP sampler collected bioaerosols, the effective volume for DNA extraction was increased 10-folds, and the overall method detection limit of microbial contaminants in bioaerosols significantly decreased. A consistent recovery of the majority of airborne bacterial populations was demonstrated by in-depth comparison of microbial composition using 16S rRNA gene high-throughput sequencing. Surface charge was shown as the deciding factor for the interaction between MNPs and microorganisms, which helps developing materials with high microbial selectivity. To our knowledge, this study is the first report using MNPs to separate diverse microbial community unselectively from a complex environmental matrix. The technique is convenient and sensitive, as well as feasible to apply in monitoring of microbial transport and other related fields. |
| first_indexed | 2024-04-12T14:03:18Z |
| format | Article |
| id | doaj.art-88d7653f35844c268464ed8b2837d135 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T14:03:18Z |
| publishDate | 2016-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-88d7653f35844c268464ed8b2837d1352022-12-22T03:30:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-12-01710.3389/fmicb.2016.01891218683Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface ChargeXin-Lei Gao0Mingfei Shao1Yi-Sheng Xu2Yi Luo3Kai Zhang4Feng Ouyang5Ji Li6Harbin Institute of TechnologyHarbin Institute of TechnologyEast China University of Science and TechnologyNankai UniversityHarbin Institute of TechnologyHarbin Institute of TechnologyHarbin Institute of TechnologyRecovering microorganisms from environmental samples is a crucial primary step for understanding microbial communities using molecular ecological approaches. It is often challenging to harvest microorganisms both efficiently and unselectively, guaranteeing a similar microbial composition between original and separated biomasses. A magnetic nanoparticles (MNPs) based method was developed to effectively separate microbial biomass from glass fiber pulp entrapped bacteria. Buffering pH and nanoparticle silica encapsulation significantly affected both biomass recovery and microbial selectivity. Under optimized conditions (using citric acid coated Fe3O4, buffering pH=2.2), the method was applied in the pretreatment of TSP sampler collected bioaerosols, the effective volume for DNA extraction was increased 10-folds, and the overall method detection limit of microbial contaminants in bioaerosols significantly decreased. A consistent recovery of the majority of airborne bacterial populations was demonstrated by in-depth comparison of microbial composition using 16S rRNA gene high-throughput sequencing. Surface charge was shown as the deciding factor for the interaction between MNPs and microorganisms, which helps developing materials with high microbial selectivity. To our knowledge, this study is the first report using MNPs to separate diverse microbial community unselectively from a complex environmental matrix. The technique is convenient and sensitive, as well as feasible to apply in monitoring of microbial transport and other related fields.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01891/fullAdsorptionmicrobial communitybioaerosolsSurface chargeMagnetic nanoparticleGlass fiber filter |
| spellingShingle | Xin-Lei Gao Mingfei Shao Yi-Sheng Xu Yi Luo Kai Zhang Feng Ouyang Ji Li Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge Frontiers in Microbiology Adsorption microbial community bioaerosols Surface charge Magnetic nanoparticle Glass fiber filter |
| title | Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge |
| title_full | Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge |
| title_fullStr | Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge |
| title_full_unstemmed | Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge |
| title_short | Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge |
| title_sort | non selective separation of bacterial cells with magnetic nanoparticles facilitated by varying surface charge |
| topic | Adsorption microbial community bioaerosols Surface charge Magnetic nanoparticle Glass fiber filter |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01891/full |
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