Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis
NF-YAs play important roles in abiotic stress. However, their characteristics and functions in abiotic stress of poplar, a model woody plant, have not been fully investigated. Here, the biological functions of PtNF-YA9 (Potri.011G101000), an NF-YA gene from Populus trichocarpa, were first fully inve...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.00954/full |
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| author | Conglong Lian Conglong Lian Conglong Lian Conglong Lian Qing Li Qing Li Qing Li Qing Li Kun Yao Kun Yao Kun Yao Kun Yao Ying Zhang Ying Zhang Ying Zhang Ying Zhang Sen Meng Sen Meng Sen Meng Sen Meng Weilun Yin Weilun Yin Weilun Yin Weilun Yin Xinli Xia Xinli Xia Xinli Xia Xinli Xia |
| author_facet | Conglong Lian Conglong Lian Conglong Lian Conglong Lian Qing Li Qing Li Qing Li Qing Li Kun Yao Kun Yao Kun Yao Kun Yao Ying Zhang Ying Zhang Ying Zhang Ying Zhang Sen Meng Sen Meng Sen Meng Sen Meng Weilun Yin Weilun Yin Weilun Yin Weilun Yin Xinli Xia Xinli Xia Xinli Xia Xinli Xia |
| author_sort | Conglong Lian |
| collection | DOAJ |
| description | NF-YAs play important roles in abiotic stress. However, their characteristics and functions in abiotic stress of poplar, a model woody plant, have not been fully investigated. Here, the biological functions of PtNF-YA9 (Potri.011G101000), an NF-YA gene from Populus trichocarpa, were first fully investigated. PtNF-YA9 is located in the nucleus. The expression of PtNF-YA9 was reduced by mannitol, NaCl, and abscisic acid (ABA). The GUS staining of ProNF-YA9::GUS transgenic lines was also reduced by mannitol treatments. In the PtNF-YA9-overexpressed Arabidopsis (OxPtNA9), OxPtNA9 lines exhibited sensitivity to simulated drought, ABA, and salinity stress during germination stage, and growth arrest emerged at post-germination stage. These phenomena might involve the ABA signaling pathway via the regulation of ABI3, ABI4, and ABI5. At vegetative stages, OxPtNA9 lines decreased in water loss via promoting stomatal closure and displayed high instantaneous water-use efficiency (WUE) of the leaf to exhibit enhanced drought tolerance. Furthermore, OxPtNA9 lines exhibited long primary root in the half-strength Murashige–Skoog agar medium supplemented with NaCl and conferred strong tolerance in the soil under salt stress. Additionally, PtNF-YA9 exhibited dwarf phenotype, short hypocotyl, small leaf area and biomass, delayed flowering, and increased chlorophyll content. Above all, our research proposes a model in which PtNF-YA9 not only plays a key role in reducing plant growth but also can play a primary role in the mechanism of an acclimatization strategy in response to adverse environmental conditions. |
| first_indexed | 2024-12-11T19:47:28Z |
| format | Article |
| id | doaj.art-88d80911a9fc457182ba272355cea551 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T19:47:28Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-88d80911a9fc457182ba272355cea5512022-12-22T00:52:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-07-01910.3389/fpls.2018.00954357559Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in ArabidopsisConglong Lian0Conglong Lian1Conglong Lian2Conglong Lian3Qing Li4Qing Li5Qing Li6Qing Li7Kun Yao8Kun Yao9Kun Yao10Kun Yao11Ying Zhang12Ying Zhang13Ying Zhang14Ying Zhang15Sen Meng16Sen Meng17Sen Meng18Sen Meng19Weilun Yin20Weilun Yin21Weilun Yin22Weilun Yin23Xinli Xia24Xinli Xia25Xinli Xia26Xinli Xia27Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, ChinaNational Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Beijing Forestry University, Beijing, ChinaNF-YAs play important roles in abiotic stress. However, their characteristics and functions in abiotic stress of poplar, a model woody plant, have not been fully investigated. Here, the biological functions of PtNF-YA9 (Potri.011G101000), an NF-YA gene from Populus trichocarpa, were first fully investigated. PtNF-YA9 is located in the nucleus. The expression of PtNF-YA9 was reduced by mannitol, NaCl, and abscisic acid (ABA). The GUS staining of ProNF-YA9::GUS transgenic lines was also reduced by mannitol treatments. In the PtNF-YA9-overexpressed Arabidopsis (OxPtNA9), OxPtNA9 lines exhibited sensitivity to simulated drought, ABA, and salinity stress during germination stage, and growth arrest emerged at post-germination stage. These phenomena might involve the ABA signaling pathway via the regulation of ABI3, ABI4, and ABI5. At vegetative stages, OxPtNA9 lines decreased in water loss via promoting stomatal closure and displayed high instantaneous water-use efficiency (WUE) of the leaf to exhibit enhanced drought tolerance. Furthermore, OxPtNA9 lines exhibited long primary root in the half-strength Murashige–Skoog agar medium supplemented with NaCl and conferred strong tolerance in the soil under salt stress. Additionally, PtNF-YA9 exhibited dwarf phenotype, short hypocotyl, small leaf area and biomass, delayed flowering, and increased chlorophyll content. Above all, our research proposes a model in which PtNF-YA9 not only plays a key role in reducing plant growth but also can play a primary role in the mechanism of an acclimatization strategy in response to adverse environmental conditions.https://www.frontiersin.org/article/10.3389/fpls.2018.00954/fullPopulus trichocarpaNF-YApost-germination growth arrestdrought toleranceplant development |
| spellingShingle | Conglong Lian Conglong Lian Conglong Lian Conglong Lian Qing Li Qing Li Qing Li Qing Li Kun Yao Kun Yao Kun Yao Kun Yao Ying Zhang Ying Zhang Ying Zhang Ying Zhang Sen Meng Sen Meng Sen Meng Sen Meng Weilun Yin Weilun Yin Weilun Yin Weilun Yin Xinli Xia Xinli Xia Xinli Xia Xinli Xia Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis Frontiers in Plant Science Populus trichocarpa NF-YA post-germination growth arrest drought tolerance plant development |
| title | Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis |
| title_full | Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis |
| title_fullStr | Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis |
| title_full_unstemmed | Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis |
| title_short | Populus trichocarpa PtNF-YA9, A Multifunctional Transcription Factor, Regulates Seed Germination, Abiotic Stress, Plant Growth and Development in Arabidopsis |
| title_sort | populus trichocarpa ptnf ya9 a multifunctional transcription factor regulates seed germination abiotic stress plant growth and development in arabidopsis |
| topic | Populus trichocarpa NF-YA post-germination growth arrest drought tolerance plant development |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.00954/full |
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