Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat
Aim: To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats. Materials and Methods: A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using...
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| Format: | Article |
| Language: | English |
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Veterinary World
2016-06-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.9/June-2016/22.pdf |
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| author | Jai Prakash Khichar Gyan Chand Gahlot Vijay Kumar Agrawal Kiran Ajay Singh Dewna Prakash Mohammad Ashraf |
| author_facet | Jai Prakash Khichar Gyan Chand Gahlot Vijay Kumar Agrawal Kiran Ajay Singh Dewna Prakash Mohammad Ashraf |
| author_sort | Jai Prakash Khichar |
| collection | DOAJ |
| description | Aim: To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.
Materials and Methods: A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.
Results: The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.
Conclusion: Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan. |
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| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-18T01:37:43Z |
| publishDate | 2016-06-01 |
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| series | Veterinary World |
| spelling | doaj.art-88d9fcac4ed14ff3a709c29dfec77ddc2022-12-21T21:25:24ZengVeterinary WorldVeterinary World0972-89882231-09162016-06-019667667910.14202/vetworld.2016.676-679Molecular characterization of exon 3 of caprine myostatin gene in Marwari goatJai Prakash Khichar0Gyan Chand Gahlot1Vijay Kumar Agrawal2Kiran3Ajay Singh Dewna4Prakash5Mohammad Ashraf6Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; Jai4me007@gmail.comMolecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; Gahlotgcbkn@rediffmail.comMolecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; drsonalvet@rediffmail.comDepartment of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Science, Bikaner, Rajasthan, India; dr.kiranbeniwal@gmail.comMolecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; ajaydewna@gmail.comMolecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; drprakashjangu@gmail.comMolecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India; aicrp@rediffmail.comAim: To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats. Materials and Methods: A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern. Results: The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively. Conclusion: Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.http://www.veterinaryworld.org/Vol.9/June-2016/22.pdfexon 3Marwari goatmyostatin genepolymerase chain reaction-single strand conformation polymorphism |
| spellingShingle | Jai Prakash Khichar Gyan Chand Gahlot Vijay Kumar Agrawal Kiran Ajay Singh Dewna Prakash Mohammad Ashraf Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat Veterinary World exon 3 Marwari goat myostatin gene polymerase chain reaction-single strand conformation polymorphism |
| title | Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat |
| title_full | Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat |
| title_fullStr | Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat |
| title_full_unstemmed | Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat |
| title_short | Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat |
| title_sort | molecular characterization of exon 3 of caprine myostatin gene in marwari goat |
| topic | exon 3 Marwari goat myostatin gene polymerase chain reaction-single strand conformation polymorphism |
| url | http://www.veterinaryworld.org/Vol.9/June-2016/22.pdf |
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