Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegra...
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MDPI AG
2021-10-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/4/4/77 |
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| author | Mikhail S. Bozhokin Svetlana A. Bozhkova Aleksandr A. Rubel Julia V. Sopova Yulia A. Nashchekina Natalya B. Bildyug Mikhail G. Khotin |
| author_facet | Mikhail S. Bozhokin Svetlana A. Bozhkova Aleksandr A. Rubel Julia V. Sopova Yulia A. Nashchekina Natalya B. Bildyug Mikhail G. Khotin |
| author_sort | Mikhail S. Bozhokin |
| collection | DOAJ |
| description | Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage. |
| first_indexed | 2024-03-10T03:27:25Z |
| format | Article |
| id | doaj.art-88daff49b2ae45c4b170ca0051a0eef4 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T03:27:25Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-88daff49b2ae45c4b170ca0051a0eef42023-11-23T09:48:18ZengMDPI AGMethods and Protocols2409-92792021-10-01447710.3390/mps4040077Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline CartilageMikhail S. Bozhokin0Svetlana A. Bozhkova1Aleksandr A. Rubel2Julia V. Sopova3Yulia A. Nashchekina4Natalya B. Bildyug5Mikhail G. Khotin6Vreden National Medical Research Center of Traumatology and Orthopedics, Academica Baykova Str., 8, 195427 St. Petersburg, RussiaVreden National Medical Research Center of Traumatology and Orthopedics, Academica Baykova Str., 8, 195427 St. Petersburg, RussiaLaboratory of Amyloid Biology, St. Petersburg State University, Universitetskaja Emb., 7/9, 199034 St. Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, RussiaDamage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.https://www.mdpi.com/2409-9279/4/4/77SEMhistologycellular engineered constructstissue engineeringhyaline cartilage |
| spellingShingle | Mikhail S. Bozhokin Svetlana A. Bozhkova Aleksandr A. Rubel Julia V. Sopova Yulia A. Nashchekina Natalya B. Bildyug Mikhail G. Khotin Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage Methods and Protocols SEM histology cellular engineered constructs tissue engineering hyaline cartilage |
| title | Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage |
| title_full | Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage |
| title_fullStr | Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage |
| title_full_unstemmed | Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage |
| title_short | Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage |
| title_sort | specificities of scanning electron microscopy and histological methods in assessing cell engineered construct effectiveness for the recovery of hyaline cartilage |
| topic | SEM histology cellular engineered constructs tissue engineering hyaline cartilage |
| url | https://www.mdpi.com/2409-9279/4/4/77 |
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