Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells
Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00116/full |
| _version_ | 1818201146109460480 |
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| author | Amy R. Donaldson Constantin Edi Tanase Dennis Awuah Pranav Vasanthi Bathrinarayanan Laurence Hall Mehdi Nikkhah Ali Khademhosseini Felicity Rose Cameron Alexander Amir M. Ghaemmaghami |
| author_facet | Amy R. Donaldson Constantin Edi Tanase Dennis Awuah Pranav Vasanthi Bathrinarayanan Laurence Hall Mehdi Nikkhah Ali Khademhosseini Felicity Rose Cameron Alexander Amir M. Ghaemmaghami |
| author_sort | Amy R. Donaldson |
| collection | DOAJ |
| description | Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned depending on the specific application. There is a growing appreciation for the interaction between biomaterials and cells of the immune system with the increasing usage of biomaterials for in vivo applications. Here, we addressed the current lack of information regarding the immune-modulatory properties of photocrosslinked GelMA. We investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform. Using lipopolysaccharide to stimulate a pro-inflammatory immune response, we found tumor necrosis factor-α (TNF-α) expression was suppressed in GelMA culture conditions. Our findings have important implications on the future use of GelMA, and potentially similar hydrogels, and highlight the significance of investigating the potential immune-modulatory properties of biomaterials. |
| first_indexed | 2024-12-12T02:48:55Z |
| format | Article |
| id | doaj.art-88dc0f951e5745eb91cd1e5c40ce8e82 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T02:48:55Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-88dc0f951e5745eb91cd1e5c40ce8e822022-12-22T00:40:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-09-01610.3389/fbioe.2018.00116374112Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear CellsAmy R. Donaldson0Constantin Edi Tanase1Dennis Awuah2Pranav Vasanthi Bathrinarayanan3Laurence Hall4Mehdi Nikkhah5Ali Khademhosseini6Felicity Rose7Cameron Alexander8Amir M. Ghaemmaghami9Immunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomImmunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomImmunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomImmunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomImmunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United StatesCenter for Minimally Invasive Therapeutics (C-MIT), California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Regenerative Medicine and Cellular Therapies, School of Pharmacy, University of Nottingham, Nottingham, United KingdomDivision of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham, United KingdomImmunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United KingdomHydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned depending on the specific application. There is a growing appreciation for the interaction between biomaterials and cells of the immune system with the increasing usage of biomaterials for in vivo applications. Here, we addressed the current lack of information regarding the immune-modulatory properties of photocrosslinked GelMA. We investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform. Using lipopolysaccharide to stimulate a pro-inflammatory immune response, we found tumor necrosis factor-α (TNF-α) expression was suppressed in GelMA culture conditions. Our findings have important implications on the future use of GelMA, and potentially similar hydrogels, and highlight the significance of investigating the potential immune-modulatory properties of biomaterials.https://www.frontiersin.org/article/10.3389/fbioe.2018.00116/fulltissue engineeringbiomaterialsimmunologyGelatin methacryloylTNF-α |
| spellingShingle | Amy R. Donaldson Constantin Edi Tanase Dennis Awuah Pranav Vasanthi Bathrinarayanan Laurence Hall Mehdi Nikkhah Ali Khademhosseini Felicity Rose Cameron Alexander Amir M. Ghaemmaghami Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells Frontiers in Bioengineering and Biotechnology tissue engineering biomaterials immunology Gelatin methacryloyl TNF-α |
| title | Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells |
| title_full | Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells |
| title_fullStr | Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells |
| title_full_unstemmed | Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells |
| title_short | Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells |
| title_sort | photocrosslinkable gelatin hydrogels modulate the production of the major pro inflammatory cytokine tnf α by human mononuclear cells |
| topic | tissue engineering biomaterials immunology Gelatin methacryloyl TNF-α |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00116/full |
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