Detection of microRNA expression in human peripheral blood microvesicles.
MicroRNAs (miRNA) are small non-coding RNAs that regulate translation of mRNA and protein. Loss or enhanced expression of miRNAs is associated with several diseases, including cancer. However, the identification of circulating miRNA in healthy donors is not well characterized. Microvesicles, also kn...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2577891?pdf=render |
| _version_ | 1818996980083326976 |
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| author | Melissa Piper Hunter Noura Ismail Xiaoli Zhang Baltazar D Aguda Eun Joo Lee Lianbo Yu Tao Xiao Jeffrey Schafer Mei-Ling Ting Lee Thomas D Schmittgen S Patrick Nana-Sinkam David Jarjoura Clay B Marsh |
| author_facet | Melissa Piper Hunter Noura Ismail Xiaoli Zhang Baltazar D Aguda Eun Joo Lee Lianbo Yu Tao Xiao Jeffrey Schafer Mei-Ling Ting Lee Thomas D Schmittgen S Patrick Nana-Sinkam David Jarjoura Clay B Marsh |
| author_sort | Melissa Piper Hunter |
| collection | DOAJ |
| description | MicroRNAs (miRNA) are small non-coding RNAs that regulate translation of mRNA and protein. Loss or enhanced expression of miRNAs is associated with several diseases, including cancer. However, the identification of circulating miRNA in healthy donors is not well characterized. Microvesicles, also known as exosomes or microparticles, circulate in the peripheral blood and can stimulate cellular signaling. In this study, we hypothesized that under normal healthy conditions, microvesicles contain miRNAs, contributing to biological homeostasis.Microvesicles were isolated from the plasma of normal healthy individuals. RNA was isolated from both the microvesicles and matched mononuclear cells and profiled for 420 known mature miRNAs by real-time PCR. Hierarchical clustering of the data sets indicated significant differences in miRNA expression between peripheral blood mononuclear cells (PBMC) and plasma microvesicles. We observed 71 miRNAs co-expressed between microvesicles and PBMC. Notably, we found 33 and 4 significantly differentially expressed miRNAs in the plasma microvesicles and mononuclear cells, respectively. Prediction of the gene targets and associated biological pathways regulated by the detected miRNAs was performed. The majority of the miRNAs expressed in the microvesicles from the blood were predicted to regulate cellular differentiation of blood cells and metabolic pathways. Interestingly, a select few miRNAs were also predicted to be important modulators of immune function.This study is the first to identify and define miRNA expression in circulating plasma microvesicles of normal subjects. The data generated from this study provides a basis for future studies to determine the predictive role of peripheral blood miRNA signatures in human disease and will enable the definition of the biological processes regulated by these miRNA. |
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| format | Article |
| id | doaj.art-88e04d6291a343ce80b00b5abaa9c190 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T21:38:21Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-88e04d6291a343ce80b00b5abaa9c1902022-12-21T19:25:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01311e369410.1371/journal.pone.0003694Detection of microRNA expression in human peripheral blood microvesicles.Melissa Piper HunterNoura IsmailXiaoli ZhangBaltazar D AgudaEun Joo LeeLianbo YuTao XiaoJeffrey SchaferMei-Ling Ting LeeThomas D SchmittgenS Patrick Nana-SinkamDavid JarjouraClay B MarshMicroRNAs (miRNA) are small non-coding RNAs that regulate translation of mRNA and protein. Loss or enhanced expression of miRNAs is associated with several diseases, including cancer. However, the identification of circulating miRNA in healthy donors is not well characterized. Microvesicles, also known as exosomes or microparticles, circulate in the peripheral blood and can stimulate cellular signaling. In this study, we hypothesized that under normal healthy conditions, microvesicles contain miRNAs, contributing to biological homeostasis.Microvesicles were isolated from the plasma of normal healthy individuals. RNA was isolated from both the microvesicles and matched mononuclear cells and profiled for 420 known mature miRNAs by real-time PCR. Hierarchical clustering of the data sets indicated significant differences in miRNA expression between peripheral blood mononuclear cells (PBMC) and plasma microvesicles. We observed 71 miRNAs co-expressed between microvesicles and PBMC. Notably, we found 33 and 4 significantly differentially expressed miRNAs in the plasma microvesicles and mononuclear cells, respectively. Prediction of the gene targets and associated biological pathways regulated by the detected miRNAs was performed. The majority of the miRNAs expressed in the microvesicles from the blood were predicted to regulate cellular differentiation of blood cells and metabolic pathways. Interestingly, a select few miRNAs were also predicted to be important modulators of immune function.This study is the first to identify and define miRNA expression in circulating plasma microvesicles of normal subjects. The data generated from this study provides a basis for future studies to determine the predictive role of peripheral blood miRNA signatures in human disease and will enable the definition of the biological processes regulated by these miRNA.http://europepmc.org/articles/PMC2577891?pdf=render |
| spellingShingle | Melissa Piper Hunter Noura Ismail Xiaoli Zhang Baltazar D Aguda Eun Joo Lee Lianbo Yu Tao Xiao Jeffrey Schafer Mei-Ling Ting Lee Thomas D Schmittgen S Patrick Nana-Sinkam David Jarjoura Clay B Marsh Detection of microRNA expression in human peripheral blood microvesicles. PLoS ONE |
| title | Detection of microRNA expression in human peripheral blood microvesicles. |
| title_full | Detection of microRNA expression in human peripheral blood microvesicles. |
| title_fullStr | Detection of microRNA expression in human peripheral blood microvesicles. |
| title_full_unstemmed | Detection of microRNA expression in human peripheral blood microvesicles. |
| title_short | Detection of microRNA expression in human peripheral blood microvesicles. |
| title_sort | detection of microrna expression in human peripheral blood microvesicles |
| url | http://europepmc.org/articles/PMC2577891?pdf=render |
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