Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids
Ionic liquids (ILs) are new green solvents, which are widely used in lignocellulosic and microalgal biorefineries. However, high-temperature operating conditions limit their application in the extraction of heat-labile algal products, such as bioactive astaxanthin. In this study, we report the techn...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/12/4/2210 |
| _version_ | 1797482944535724032 |
|---|---|
| author | Nakyeong Lee Aditya Lakshmi Narasimhan Gyuseop Moon Young-Eun Kim Myeonghwa Park Bolam Kim Rendi Mahadi Sungwook Chung You-Kwan Oh |
| author_facet | Nakyeong Lee Aditya Lakshmi Narasimhan Gyuseop Moon Young-Eun Kim Myeonghwa Park Bolam Kim Rendi Mahadi Sungwook Chung You-Kwan Oh |
| author_sort | Nakyeong Lee |
| collection | DOAJ |
| description | Ionic liquids (ILs) are new green solvents, which are widely used in lignocellulosic and microalgal biorefineries. However, high-temperature operating conditions limit their application in the extraction of heat-labile algal products, such as bioactive astaxanthin. In this study, we report the technical feasibility of room-temperature astaxanthin extraction from <i>Haematococcus lacustris</i> cysts with a thick and complex cell wall structure, by combining ultrathin α-quartz nanoplates (NPLs) with ethyl-3-methylimidazolium ([Emim])-based ILs. When four different [Emim]-based ILs with thiocyanate (SCN), diethylphosphate (DEP), HSO<sub>4</sub>, and Cl anions were applied to 90-day-old <i>H. lacustris</i> cysts at room temperature (~28 °C), the astaxanthin extraction efficiency was as low as 9.6–14.2%. Under sonication, α-quartz NPLs disrupted the cyst cell wall for a short duration (5 min). The astaxanthin extraction efficacies of a subsequent IL treatment improved significantly to 49.8% for [Emim] SCN, 60.0% for [Emim] DEP, 80.7% for [Emim] HSO<sub>4</sub>, and 74.3% for [Emim] Cl ions, which were 4.4, 6.1, 8.4, and 5.2 times higher than the extraction efficacy of only ILs, respectively. This finding suggests that α-quartz NPLs can serve as powerful cell-wall-disrupting agents for the room-temperature IL-mediated extraction of astaxanthin from robust algal cyst cells. |
| first_indexed | 2024-03-09T22:39:48Z |
| format | Article |
| id | doaj.art-88e07c7728754b73a22da3bdca540527 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T22:39:48Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-88e07c7728754b73a22da3bdca5405272023-11-23T18:41:08ZengMDPI AGApplied Sciences2076-34172022-02-01124221010.3390/app12042210Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic LiquidsNakyeong Lee0Aditya Lakshmi Narasimhan1Gyuseop Moon2Young-Eun Kim3Myeonghwa Park4Bolam Kim5Rendi Mahadi6Sungwook Chung7You-Kwan Oh8Institute for Environment & Energy, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaInstitute for Environment & Energy, Pusan National University, Busan 46241, KoreaInstitute for Environment & Energy, Pusan National University, Busan 46241, KoreaSchool of Chemical Engineering, Pusan National University, Busan 46241, KoreaInstitute for Environment & Energy, Pusan National University, Busan 46241, KoreaInstitute for Environment & Energy, Pusan National University, Busan 46241, KoreaIonic liquids (ILs) are new green solvents, which are widely used in lignocellulosic and microalgal biorefineries. However, high-temperature operating conditions limit their application in the extraction of heat-labile algal products, such as bioactive astaxanthin. In this study, we report the technical feasibility of room-temperature astaxanthin extraction from <i>Haematococcus lacustris</i> cysts with a thick and complex cell wall structure, by combining ultrathin α-quartz nanoplates (NPLs) with ethyl-3-methylimidazolium ([Emim])-based ILs. When four different [Emim]-based ILs with thiocyanate (SCN), diethylphosphate (DEP), HSO<sub>4</sub>, and Cl anions were applied to 90-day-old <i>H. lacustris</i> cysts at room temperature (~28 °C), the astaxanthin extraction efficiency was as low as 9.6–14.2%. Under sonication, α-quartz NPLs disrupted the cyst cell wall for a short duration (5 min). The astaxanthin extraction efficacies of a subsequent IL treatment improved significantly to 49.8% for [Emim] SCN, 60.0% for [Emim] DEP, 80.7% for [Emim] HSO<sub>4</sub>, and 74.3% for [Emim] Cl ions, which were 4.4, 6.1, 8.4, and 5.2 times higher than the extraction efficacy of only ILs, respectively. This finding suggests that α-quartz NPLs can serve as powerful cell-wall-disrupting agents for the room-temperature IL-mediated extraction of astaxanthin from robust algal cyst cells.https://www.mdpi.com/2076-3417/12/4/2210microalgae<i>Haematococcus lacustris</i>astaxanthinionic liquidnanoplatecell disruption |
| spellingShingle | Nakyeong Lee Aditya Lakshmi Narasimhan Gyuseop Moon Young-Eun Kim Myeonghwa Park Bolam Kim Rendi Mahadi Sungwook Chung You-Kwan Oh Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids Applied Sciences microalgae <i>Haematococcus lacustris</i> astaxanthin ionic liquid nanoplate cell disruption |
| title | Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids |
| title_full | Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids |
| title_fullStr | Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids |
| title_full_unstemmed | Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids |
| title_short | Room-Temperature Cell Disruption and Astaxanthin Recovery from <i>Haematococcus lacustris</i> Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids |
| title_sort | room temperature cell disruption and astaxanthin recovery from i haematococcus lacustris i cysts using ultrathin α quartz nanoplates and ionic liquids |
| topic | microalgae <i>Haematococcus lacustris</i> astaxanthin ionic liquid nanoplate cell disruption |
| url | https://www.mdpi.com/2076-3417/12/4/2210 |
| work_keys_str_mv | AT nakyeonglee roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT adityalakshminarasimhan roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT gyuseopmoon roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT youngeunkim roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT myeonghwapark roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT bolamkim roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT rendimahadi roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT sungwookchung roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids AT youkwanoh roomtemperaturecelldisruptionandastaxanthinrecoveryfromihaematococcuslacustrisicystsusingultrathinaquartznanoplatesandionicliquids |