Single transmembrane peptide DinQ modulates membrane-dependent activities.
The functions of several SOS regulated genes in Escherichia coli are still unknown, including dinQ. In this work we characterize dinQ and two small RNAs, agrA and agrB, with antisense complementarity to dinQ. Northern analysis revealed five dinQ transcripts, but only one transcript (+44) is actively...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3567139?pdf=render |
| _version_ | 1818996550345424896 |
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| author | Ragnhild Weel-Sneve Knut Ivan Kristiansen Ingvild Odsbu Bjørn Dalhus James Booth Torbjørn Rognes Kirsten Skarstad Magnar Bjørås |
| author_facet | Ragnhild Weel-Sneve Knut Ivan Kristiansen Ingvild Odsbu Bjørn Dalhus James Booth Torbjørn Rognes Kirsten Skarstad Magnar Bjørås |
| author_sort | Ragnhild Weel-Sneve |
| collection | DOAJ |
| description | The functions of several SOS regulated genes in Escherichia coli are still unknown, including dinQ. In this work we characterize dinQ and two small RNAs, agrA and agrB, with antisense complementarity to dinQ. Northern analysis revealed five dinQ transcripts, but only one transcript (+44) is actively translated. The +44 dinQ transcript translates into a toxic single transmembrane peptide localized in the inner membrane. AgrB regulates dinQ RNA by RNA interference to counteract DinQ toxicity. Thus the dinQ-agr locus shows the classical features of a type I TA system and has many similarities to the tisB-istR locus. DinQ overexpression depolarizes the cell membrane and decreases the intracellular ATP concentration, demonstrating that DinQ can modulate membrane-dependent processes. Augmented DinQ strongly inhibits marker transfer by Hfr conjugation, indicating a role in recombination. Furthermore, DinQ affects transformation of nucleoid morphology in response to UV damage. We hypothesize that DinQ is a transmembrane peptide that modulates membrane-dependent activities such as nucleoid compaction and recombination. |
| first_indexed | 2024-12-20T21:31:31Z |
| format | Article |
| id | doaj.art-88e33cdc64ad43078db8c48c508a22bf |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-20T21:31:31Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-88e33cdc64ad43078db8c48c508a22bf2022-12-21T19:26:03ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-01-0192e100326010.1371/journal.pgen.1003260Single transmembrane peptide DinQ modulates membrane-dependent activities.Ragnhild Weel-SneveKnut Ivan KristiansenIngvild OdsbuBjørn DalhusJames BoothTorbjørn RognesKirsten SkarstadMagnar BjøråsThe functions of several SOS regulated genes in Escherichia coli are still unknown, including dinQ. In this work we characterize dinQ and two small RNAs, agrA and agrB, with antisense complementarity to dinQ. Northern analysis revealed five dinQ transcripts, but only one transcript (+44) is actively translated. The +44 dinQ transcript translates into a toxic single transmembrane peptide localized in the inner membrane. AgrB regulates dinQ RNA by RNA interference to counteract DinQ toxicity. Thus the dinQ-agr locus shows the classical features of a type I TA system and has many similarities to the tisB-istR locus. DinQ overexpression depolarizes the cell membrane and decreases the intracellular ATP concentration, demonstrating that DinQ can modulate membrane-dependent processes. Augmented DinQ strongly inhibits marker transfer by Hfr conjugation, indicating a role in recombination. Furthermore, DinQ affects transformation of nucleoid morphology in response to UV damage. We hypothesize that DinQ is a transmembrane peptide that modulates membrane-dependent activities such as nucleoid compaction and recombination.http://europepmc.org/articles/PMC3567139?pdf=render |
| spellingShingle | Ragnhild Weel-Sneve Knut Ivan Kristiansen Ingvild Odsbu Bjørn Dalhus James Booth Torbjørn Rognes Kirsten Skarstad Magnar Bjørås Single transmembrane peptide DinQ modulates membrane-dependent activities. PLoS Genetics |
| title | Single transmembrane peptide DinQ modulates membrane-dependent activities. |
| title_full | Single transmembrane peptide DinQ modulates membrane-dependent activities. |
| title_fullStr | Single transmembrane peptide DinQ modulates membrane-dependent activities. |
| title_full_unstemmed | Single transmembrane peptide DinQ modulates membrane-dependent activities. |
| title_short | Single transmembrane peptide DinQ modulates membrane-dependent activities. |
| title_sort | single transmembrane peptide dinq modulates membrane dependent activities |
| url | http://europepmc.org/articles/PMC3567139?pdf=render |
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