Activation of the cGAS–STING signaling pathway in adenomyosis patients
Abstract Objective Adenomyosis is characterized by the presence of endometrium or endometrium‐like glands and stroma within the myometrium. In this study, we aimed to investigate whether the cGAS–STING pathway was activated and correlated with clinical outcomes in adenomyosis patients. Materials and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.452 |
| _version_ | 1818972734793711616 |
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| author | Yun Lin Luying Wang Mingzhu Ye Ke‐nan Yu Xin Sun Min Xue Xinliang Deng |
| author_facet | Yun Lin Luying Wang Mingzhu Ye Ke‐nan Yu Xin Sun Min Xue Xinliang Deng |
| author_sort | Yun Lin |
| collection | DOAJ |
| description | Abstract Objective Adenomyosis is characterized by the presence of endometrium or endometrium‐like glands and stroma within the myometrium. In this study, we aimed to investigate whether the cGAS–STING pathway was activated and correlated with clinical outcomes in adenomyosis patients. Materials and Methods Twenty patients diagnosed with adenomyosis and 10 patients diagnosed with cervical intraepithelial neoplasia grade 3 (CIN‐3) but no adenomyosis were enrolled in this study. Specimens were collected during surgery from August 2017 to December 2017 at Third Xiangya Hospital. The messenger RNA (mRNA) and protein levels of key cGAS–STING pathway factors in uterine tissue were detected by real‐time reverse‐transcription polymerase chain reaction and immunohistochemistry, respectively. The correlations of gene expression and clinical outcomes, including dysmenorrhea and uterine volume, were analyzed. Results The cGAS, STING, TANK‐binding kinase 1 (TBK‐1), interferon‐α (IFN‐α), IFN‐β, and tumor necrosis factor‐α (TNF‐α) mRNA and protein levels in the ectopic endometrial tissue from adenomyosis patients were significantly higher compared with that from the controls in endometrium (p < .05). cGAS and STING gene expression were correlated with TBK‐1, IFN‐β, and TNF‐α expression (p < .05). Importantly, TBK‐1 and TNF‐α expression were correlated with the clinical outcome of dysmenorrhea (p < .05). Conclusion Our study reveals that the cGAS–STING pathway is activated in adenomyosis patients and its activation is subsequently correlated with clinical outcomes, which suggests that the cGAS–STING pathway may contribute to adenomyosis pathogenesis. |
| first_indexed | 2024-12-20T15:12:59Z |
| format | Article |
| id | doaj.art-88e3b5578be346b581bb0c28501ed7b5 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-12-20T15:12:59Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-88e3b5578be346b581bb0c28501ed7b52022-12-21T19:36:16ZengWileyImmunity, Inflammation and Disease2050-45272021-09-019393294210.1002/iid3.452Activation of the cGAS–STING signaling pathway in adenomyosis patientsYun Lin0Luying Wang1Mingzhu Ye2Ke‐nan Yu3Xin Sun4Min Xue5Xinliang Deng6Department of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaDepartment of Obstetrics and Gynecology The 3rd Xiangya Hospital of Central South University Changsha Hunan ChinaAbstract Objective Adenomyosis is characterized by the presence of endometrium or endometrium‐like glands and stroma within the myometrium. In this study, we aimed to investigate whether the cGAS–STING pathway was activated and correlated with clinical outcomes in adenomyosis patients. Materials and Methods Twenty patients diagnosed with adenomyosis and 10 patients diagnosed with cervical intraepithelial neoplasia grade 3 (CIN‐3) but no adenomyosis were enrolled in this study. Specimens were collected during surgery from August 2017 to December 2017 at Third Xiangya Hospital. The messenger RNA (mRNA) and protein levels of key cGAS–STING pathway factors in uterine tissue were detected by real‐time reverse‐transcription polymerase chain reaction and immunohistochemistry, respectively. The correlations of gene expression and clinical outcomes, including dysmenorrhea and uterine volume, were analyzed. Results The cGAS, STING, TANK‐binding kinase 1 (TBK‐1), interferon‐α (IFN‐α), IFN‐β, and tumor necrosis factor‐α (TNF‐α) mRNA and protein levels in the ectopic endometrial tissue from adenomyosis patients were significantly higher compared with that from the controls in endometrium (p < .05). cGAS and STING gene expression were correlated with TBK‐1, IFN‐β, and TNF‐α expression (p < .05). Importantly, TBK‐1 and TNF‐α expression were correlated with the clinical outcome of dysmenorrhea (p < .05). Conclusion Our study reveals that the cGAS–STING pathway is activated in adenomyosis patients and its activation is subsequently correlated with clinical outcomes, which suggests that the cGAS–STING pathway may contribute to adenomyosis pathogenesis.https://doi.org/10.1002/iid3.452adenomyosisendometrium cGASSTING |
| spellingShingle | Yun Lin Luying Wang Mingzhu Ye Ke‐nan Yu Xin Sun Min Xue Xinliang Deng Activation of the cGAS–STING signaling pathway in adenomyosis patients Immunity, Inflammation and Disease adenomyosis endometrium cGAS STING |
| title | Activation of the cGAS–STING signaling pathway in adenomyosis patients |
| title_full | Activation of the cGAS–STING signaling pathway in adenomyosis patients |
| title_fullStr | Activation of the cGAS–STING signaling pathway in adenomyosis patients |
| title_full_unstemmed | Activation of the cGAS–STING signaling pathway in adenomyosis patients |
| title_short | Activation of the cGAS–STING signaling pathway in adenomyosis patients |
| title_sort | activation of the cgas sting signaling pathway in adenomyosis patients |
| topic | adenomyosis endometrium cGAS STING |
| url | https://doi.org/10.1002/iid3.452 |
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