Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil
The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the sou...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100009&lng=en&tlng=en |
| _version_ | 1797721283703603200 |
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| author | Diogo André Pilger Vlademir Vicente Cantarelli Sérgio Luis Amantea Sandra Leistner-Segal |
| author_facet | Diogo André Pilger Vlademir Vicente Cantarelli Sérgio Luis Amantea Sandra Leistner-Segal |
| author_sort | Diogo André Pilger |
| collection | DOAJ |
| description | The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents. |
| first_indexed | 2024-03-12T09:31:17Z |
| format | Article |
| id | doaj.art-88e5cccf6d914d778ef104a9ded1d686 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:17Z |
| publishDate | 2011-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-88e5cccf6d914d778ef104a9ded1d6862023-09-02T13:54:11ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-02-011061566010.1590/S0074-02762011000100009S0074-02762011000100009Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern BrazilDiogo André Pilger0Vlademir Vicente Cantarelli1Sérgio Luis Amantea2Sandra Leistner-Segal3Faculdade de FarmáciaUniversidade FeevaleSanta Casa de Misericórdia de Porto AlegreUniversidade Federal do Rio Grande do SulThe introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100009&lng=en&tlng=enhuman metapneumovirushuman bocavirusreal-time PCRrespiratory tract infection |
| spellingShingle | Diogo André Pilger Vlademir Vicente Cantarelli Sérgio Luis Amantea Sandra Leistner-Segal Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil Memorias do Instituto Oswaldo Cruz human metapneumovirus human bocavirus real-time PCR respiratory tract infection |
| title | Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil |
| title_full | Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil |
| title_fullStr | Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil |
| title_full_unstemmed | Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil |
| title_short | Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil |
| title_sort | detection of human bocavirus and human metapneumovirus by real time pcr from patients with respiratory symptoms in southern brazil |
| topic | human metapneumovirus human bocavirus real-time PCR respiratory tract infection |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100009&lng=en&tlng=en |
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