Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA
Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/283 |
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| author | Haixing Li Lingqin Wang Lijuan Nie Xiaohua Liu Jinheng Fu |
| author_facet | Haixing Li Lingqin Wang Lijuan Nie Xiaohua Liu Jinheng Fu |
| author_sort | Haixing Li |
| collection | DOAJ |
| description | Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process of <i>Levilactobacillus brevis</i> CD0817. The results demonstrated that SINICS is an attractive alternative to the available ninhydrin-based colorimetric methods. |
| first_indexed | 2024-03-11T08:26:23Z |
| format | Article |
| id | doaj.art-88ef256af8ca4c27b576678e8d78982c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:23Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-88ef256af8ca4c27b576678e8d78982c2023-11-16T22:05:26ZengMDPI AGMetabolites2218-19892023-02-0113228310.3390/metabo13020283Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABAHaixing Li0Lingqin Wang1Lijuan Nie2Xiaohua Liu3Jinheng Fu4State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaGamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process of <i>Levilactobacillus brevis</i> CD0817. The results demonstrated that SINICS is an attractive alternative to the available ninhydrin-based colorimetric methods.https://www.mdpi.com/2218-1989/13/2/283ninhydrin-based chromogenic systemcolorimetry analysisrelative quantitationgamma-aminobutyric acidamino acids |
| spellingShingle | Haixing Li Lingqin Wang Lijuan Nie Xiaohua Liu Jinheng Fu Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA Metabolites ninhydrin-based chromogenic system colorimetry analysis relative quantitation gamma-aminobutyric acid amino acids |
| title | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
| title_full | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
| title_fullStr | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
| title_full_unstemmed | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
| title_short | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
| title_sort | sensitivity intensified ninhydrin based chromogenic system by ethanol ethyl acetate application to relative quantitation of gaba |
| topic | ninhydrin-based chromogenic system colorimetry analysis relative quantitation gamma-aminobutyric acid amino acids |
| url | https://www.mdpi.com/2218-1989/13/2/283 |
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