Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. H...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2021-11-01
|
| Series: | ZooKeys |
| Online Access: | https://zookeys.pensoft.net/article/73075/download/pdf/ |
| _version_ | 1811329211953053696 |
|---|---|
| author | Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa |
| author_facet | Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa |
| author_sort | Gabina Calderón-Rosete |
| collection | DOAJ |
| description | Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarus clarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94–100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P. clarkii use biosynthesis pathways and phototransduction cascades commons. |
| first_indexed | 2024-04-13T15:39:47Z |
| format | Article |
| id | doaj.art-88ef2d969ed9487993356fa369f1b164 |
| institution | Directory Open Access Journal |
| issn | 1313-2970 |
| language | English |
| last_indexed | 2024-04-13T15:39:47Z |
| publishDate | 2021-11-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | ZooKeys |
| spelling | doaj.art-88ef2d969ed9487993356fa369f1b1642022-12-22T02:41:10ZengPensoft PublishersZooKeys1313-29702021-11-01107210712710.3897/zookeys.1072.7307573075Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkiiGabina Calderón-Rosete0Juan Antonio González-Barrios1Celia Piña-Leyva2Hayde Nallely Moreno-Sandoval3Manuel Lara-Lozano4Leonardo Rodríguez-Sosa5Universidad Nacional Autónoma de MéxicoLaboratorio de Medicina GenómicaCentro de Investigación y Estudios AvanzadosLaboratorio de Medicina GenómicaLaboratorio de Medicina GenómicaUniversidad Nacional Autónoma de MéxicoCrayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarus clarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94–100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P. clarkii use biosynthesis pathways and phototransduction cascades commons.https://zookeys.pensoft.net/article/73075/download/pdf/ |
| spellingShingle | Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii ZooKeys |
| title | Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii |
| title_full | Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii |
| title_fullStr | Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii |
| title_full_unstemmed | Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii |
| title_short | Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii |
| title_sort | transcriptional identification of genes light interacting in the extraretinal photoreceptors of the crayfish procambarus clarkii |
| url | https://zookeys.pensoft.net/article/73075/download/pdf/ |
| work_keys_str_mv | AT gabinacalderonrosete transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii AT juanantoniogonzalezbarrios transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii AT celiapinaleyva transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii AT haydenallelymorenosandoval transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii AT manuellaralozano transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii AT leonardorodriguezsosa transcriptionalidentificationofgeneslightinteractingintheextraretinalphotoreceptorsofthecrayfishprocambarusclarkii |