Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts
This study aimed to determine the chemical composition of different types of tissue of <i>Cedrus brevifolia</i> Henry (Pinaceae) methanolic extracts, namely needles, twigs, branches, and bark. <i>Cedrus brevifolia</i> is a narrow endemic coniferous tree species of Cyprus, gro...
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2717 |
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| author | Despina Charalambous Nicolas-George Homer Eliades Michalis Christoforou Eleni Kakouri Charalabos Kanakis Petros A. Tarantilis Maria Pantelidou |
| author_facet | Despina Charalambous Nicolas-George Homer Eliades Michalis Christoforou Eleni Kakouri Charalabos Kanakis Petros A. Tarantilis Maria Pantelidou |
| author_sort | Despina Charalambous |
| collection | DOAJ |
| description | This study aimed to determine the chemical composition of different types of tissue of <i>Cedrus brevifolia</i> Henry (Pinaceae) methanolic extracts, namely needles, twigs, branches, and bark. <i>Cedrus brevifolia</i> is a narrow endemic coniferous tree species of Cyprus, growing in a sole population in the mountainous area of Paphos Forest. Chemical analysis of the extracts was performed using liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The majority of the 36 compounds tentatively identified belonged to the flavonoids family. The extract of needles was the richest extract in terms of secondary metabolites. The extracts were studied for their antioxidant activity using the DPPH free radical scavenging assay. Additionally, the antibacterial activity was evaluated by determining both the minimum inhibitory concentration and the minimum bactericidal concentration against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. All extracts demonstrated antioxidant property, while bark gave the highest antioxidant capacity (IC<sub>50</sub> value of 0.011 mg/mL) compared to the other tissues. Antibacterial activity was observed against both types of bacteria, with the extract of branches presenting the strongest activity against <i>S. aureus</i> (MIC, 0.097 mg/mL and MBC, 0.195 mg/mL). This is the first time that extracts of needles, twigs, branches, and bark of <i>C. brevifolia</i> are compared regarding their chemical composition as well as their antimicrobial and antioxidant properties. |
| first_indexed | 2024-03-10T03:54:44Z |
| format | Article |
| id | doaj.art-88f121de233e47a29a03c9e75a8edae4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:54:44Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-88f121de233e47a29a03c9e75a8edae42023-11-23T08:48:33ZengMDPI AGMolecules1420-30492022-04-01279271710.3390/molecules27092717Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry ExtractsDespina Charalambous0Nicolas-George Homer Eliades1Michalis Christoforou2Eleni Kakouri3Charalabos Kanakis4Petros A. Tarantilis5Maria Pantelidou6Department of Pharmacy, School of Health Sciences, Frederick University, 7, Y. Frederickou Str., Pallouriotissa, Nicosia 1036, CyprusNature Conservation Unit, Frederick University, Nicosia 1036, CyprusDepartment of Pharmacy, School of Health Sciences, Frederick University, 7, Y. Frederickou Str., Pallouriotissa, Nicosia 1036, CyprusLaboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceLaboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceLaboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceDepartment of Pharmacy, School of Health Sciences, Frederick University, 7, Y. Frederickou Str., Pallouriotissa, Nicosia 1036, CyprusThis study aimed to determine the chemical composition of different types of tissue of <i>Cedrus brevifolia</i> Henry (Pinaceae) methanolic extracts, namely needles, twigs, branches, and bark. <i>Cedrus brevifolia</i> is a narrow endemic coniferous tree species of Cyprus, growing in a sole population in the mountainous area of Paphos Forest. Chemical analysis of the extracts was performed using liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The majority of the 36 compounds tentatively identified belonged to the flavonoids family. The extract of needles was the richest extract in terms of secondary metabolites. The extracts were studied for their antioxidant activity using the DPPH free radical scavenging assay. Additionally, the antibacterial activity was evaluated by determining both the minimum inhibitory concentration and the minimum bactericidal concentration against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. All extracts demonstrated antioxidant property, while bark gave the highest antioxidant capacity (IC<sub>50</sub> value of 0.011 mg/mL) compared to the other tissues. Antibacterial activity was observed against both types of bacteria, with the extract of branches presenting the strongest activity against <i>S. aureus</i> (MIC, 0.097 mg/mL and MBC, 0.195 mg/mL). This is the first time that extracts of needles, twigs, branches, and bark of <i>C. brevifolia</i> are compared regarding their chemical composition as well as their antimicrobial and antioxidant properties.https://www.mdpi.com/1420-3049/27/9/2717<i>Cedrus brevifolia</i>CyprusantioxidantantibacterialconiferousLC/Q-TOF/HRMS |
| spellingShingle | Despina Charalambous Nicolas-George Homer Eliades Michalis Christoforou Eleni Kakouri Charalabos Kanakis Petros A. Tarantilis Maria Pantelidou Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts Molecules <i>Cedrus brevifolia</i> Cyprus antioxidant antibacterial coniferous LC/Q-TOF/HRMS |
| title | Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts |
| title_full | Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts |
| title_fullStr | Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts |
| title_full_unstemmed | Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts |
| title_short | Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of <i>Cedrus brevifolia</i> Henry Extracts |
| title_sort | chemical characterization antioxidant and antimicrobial properties of different types of tissue of i cedrus brevifolia i henry extracts |
| topic | <i>Cedrus brevifolia</i> Cyprus antioxidant antibacterial coniferous LC/Q-TOF/HRMS |
| url | https://www.mdpi.com/1420-3049/27/9/2717 |
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