Methods of preparing flower stem samples for scanning electron microscopy
Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector, helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool that allows viewing of flower structures and a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Floricultura e Plantas Ornamentais
2015-04-01
|
| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/771 |
| _version_ | 1797797412636459008 |
|---|---|
| author | Poliana Cristina Spricigo Jéssica Prada Trento Joana Dias Bresolin Viviane Faria Soares Viviane Faria Soares Luiz Francisco De Mattêo Ferraz Marcos David Ferreira |
| author_facet | Poliana Cristina Spricigo Jéssica Prada Trento Joana Dias Bresolin Viviane Faria Soares Viviane Faria Soares Luiz Francisco De Mattêo Ferraz Marcos David Ferreira |
| author_sort | Poliana Cristina Spricigo |
| collection | DOAJ |
| description | Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector, helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool that allows viewing of flower structures and also microorganisms. The aim of this study was to evaluate methods of preparing flower stem samples for viewing in SEM as a support for studies on postharvest cut flowers. Ways of cutting, fixing, and drying samples were tested. Cutting with a stainless steel blade and through freeze-fracture were tested; fixation was carried out without the use of osmium tetroxide (OsO4); and drying of the samples was performed through freeze-drying and through critical point dryingwithCO2. Cutting with a stainless steel blade proved to be a satisfactory method for stem samples, with low cost and simple application compared to freeze-fracturing. Good fixation and high image contrast were obtained without the use of osmium tetroxide, thus avoiding the use of this toxic compound. Freeze-drying allowed the structure and morphological composition to be viewed, while critical point drying withCO2 preserved the microorganisms present in the samples. |
| first_indexed | 2024-03-13T03:47:57Z |
| format | Article |
| id | doaj.art-88f19031b25244769e7e07183218c1d9 |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-13T03:47:57Z |
| publishDate | 2015-04-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-88f19031b25244769e7e07183218c1d92023-06-22T20:36:04ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2015-04-0121110.14295/rbho.v21i1.771458Methods of preparing flower stem samples for scanning electron microscopyPoliana Cristina SpricigoJéssica Prada TrentoJoana Dias BresolinViviane Faria SoaresViviane Faria SoaresLuiz Francisco De Mattêo FerrazMarcos David FerreiraBrazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector, helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool that allows viewing of flower structures and also microorganisms. The aim of this study was to evaluate methods of preparing flower stem samples for viewing in SEM as a support for studies on postharvest cut flowers. Ways of cutting, fixing, and drying samples were tested. Cutting with a stainless steel blade and through freeze-fracture were tested; fixation was carried out without the use of osmium tetroxide (OsO4); and drying of the samples was performed through freeze-drying and through critical point dryingwithCO2. Cutting with a stainless steel blade proved to be a satisfactory method for stem samples, with low cost and simple application compared to freeze-fracturing. Good fixation and high image contrast were obtained without the use of osmium tetroxide, thus avoiding the use of this toxic compound. Freeze-drying allowed the structure and morphological composition to be viewed, while critical point drying withCO2 preserved the microorganisms present in the samples.https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/771Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sectorhelping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool t |
| spellingShingle | Poliana Cristina Spricigo Jéssica Prada Trento Joana Dias Bresolin Viviane Faria Soares Viviane Faria Soares Luiz Francisco De Mattêo Ferraz Marcos David Ferreira Methods of preparing flower stem samples for scanning electron microscopy Ornamental Horticulture Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool t |
| title | Methods of preparing flower stem samples for scanning electron microscopy |
| title_full | Methods of preparing flower stem samples for scanning electron microscopy |
| title_fullStr | Methods of preparing flower stem samples for scanning electron microscopy |
| title_full_unstemmed | Methods of preparing flower stem samples for scanning electron microscopy |
| title_short | Methods of preparing flower stem samples for scanning electron microscopy |
| title_sort | methods of preparing flower stem samples for scanning electron microscopy |
| topic | Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool t |
| url | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/771 |
| work_keys_str_mv | AT polianacristinaspricigo methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT jessicapradatrento methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT joanadiasbresolin methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT vivianefariasoares methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT vivianefariasoares methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT luizfranciscodematteoferraz methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy AT marcosdavidferreira methodsofpreparingflowerstemsamplesforscanningelectronmicroscopy |