Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice
Bacterial blight (BB) disease, caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>), is among the major factors that can cause rice yields to decrease. To address BB disease, researchers have been looking for ways to change pesticides and cultivation...
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/11/1404 |
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| author | Jae-Ryoung Park Chang-Min Lee Hyeonso Ji Man-Kee Baek Jeonghwan Seo O-Young Jeong Hyun-Su Park |
| author_facet | Jae-Ryoung Park Chang-Min Lee Hyeonso Ji Man-Kee Baek Jeonghwan Seo O-Young Jeong Hyun-Su Park |
| author_sort | Jae-Ryoung Park |
| collection | DOAJ |
| description | Bacterial blight (BB) disease, caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>), is among the major factors that can cause rice yields to decrease. To address BB disease, researchers have been looking for ways to change pesticides and cultivation methods, but developing resistant cultivars is the most effective method. However, the resistance and genetic factors of cultivars may be destroyed due to the emergence of new <i>Xoo</i> species caused by recent and rapid climate changes. Therefore, breeders need to identify resistance genes that can be sustained during unpredictable climate changes and utilized for breeding. Here, <i>qBBR11</i>, a quantitative trait locus (QTL) for resistance to BB disease, was detected in KJ (Korea <i>Japonica</i> varieties) 11_067 to KJ11_068 on chromosome 11 in a population derived by crossing JJ (Jeonju) 623 and HR(High resistant)27,195, which possess similar genetic backgrounds but different degrees of resistance to BB disease. <i>qBBR11</i> was reduced from 18.49–18.69 Mbp of chromosome 11 to 200 kbp segment franked. In this region, 16 candidate genes were detected, and we identified 24 moderate-impact variations and four high-impact variations. In particular, high-impact variations were detected in <i>Os11g0517800</i> which encode the domain region of <i>GCN2</i> which is the eIF-2-alpha kinase associated with the resistance of abiotic/biotic stress in rice. In JJ623, which is moderately resistant to BB disease, a stop codon was created due to single nucleotide polymorphism (SNP). Therefore, compared with HR27195, JJ623 has weaker resistance to BB disease, though the two have similar genetic backgrounds. The results suggest that variation in the <i>qBBR11</i> region regulates an important role in improving resistance to BB diseases, and <i>qBBR11</i> is useful in providing an important resource for marker-assisted selection to improve mechanisms of resistance to BB disease. |
| first_indexed | 2024-03-10T00:58:49Z |
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| language | English |
| last_indexed | 2024-03-10T00:58:49Z |
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| spelling | doaj.art-88f307c6d47e4b7f9665ef15af8058922023-11-23T14:38:05ZengMDPI AGPlants2223-77472022-05-011111140410.3390/plants11111404Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in RiceJae-Ryoung Park0Chang-Min Lee1Hyeonso Ji2Man-Kee Baek3Jeonghwan Seo4O-Young Jeong5Hyun-Su Park6Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Jeonju 54874, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaCrop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaBacterial blight (BB) disease, caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>), is among the major factors that can cause rice yields to decrease. To address BB disease, researchers have been looking for ways to change pesticides and cultivation methods, but developing resistant cultivars is the most effective method. However, the resistance and genetic factors of cultivars may be destroyed due to the emergence of new <i>Xoo</i> species caused by recent and rapid climate changes. Therefore, breeders need to identify resistance genes that can be sustained during unpredictable climate changes and utilized for breeding. Here, <i>qBBR11</i>, a quantitative trait locus (QTL) for resistance to BB disease, was detected in KJ (Korea <i>Japonica</i> varieties) 11_067 to KJ11_068 on chromosome 11 in a population derived by crossing JJ (Jeonju) 623 and HR(High resistant)27,195, which possess similar genetic backgrounds but different degrees of resistance to BB disease. <i>qBBR11</i> was reduced from 18.49–18.69 Mbp of chromosome 11 to 200 kbp segment franked. In this region, 16 candidate genes were detected, and we identified 24 moderate-impact variations and four high-impact variations. In particular, high-impact variations were detected in <i>Os11g0517800</i> which encode the domain region of <i>GCN2</i> which is the eIF-2-alpha kinase associated with the resistance of abiotic/biotic stress in rice. In JJ623, which is moderately resistant to BB disease, a stop codon was created due to single nucleotide polymorphism (SNP). Therefore, compared with HR27195, JJ623 has weaker resistance to BB disease, though the two have similar genetic backgrounds. The results suggest that variation in the <i>qBBR11</i> region regulates an important role in improving resistance to BB diseases, and <i>qBBR11</i> is useful in providing an important resource for marker-assisted selection to improve mechanisms of resistance to BB disease.https://www.mdpi.com/2223-7747/11/11/1404bacterial blight diseasericebreedingQTLSNP |
| spellingShingle | Jae-Ryoung Park Chang-Min Lee Hyeonso Ji Man-Kee Baek Jeonghwan Seo O-Young Jeong Hyun-Su Park Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice Plants bacterial blight disease rice breeding QTL SNP |
| title | Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice |
| title_full | Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice |
| title_fullStr | Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice |
| title_full_unstemmed | Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice |
| title_short | Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice |
| title_sort | characterization and qtl mapping of a major field resistance locus for bacterial blight in rice |
| topic | bacterial blight disease rice breeding QTL SNP |
| url | https://www.mdpi.com/2223-7747/11/11/1404 |
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