Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation
Jerusalem artichoke tuber (JAT) is rich in inulin and it is considered as one of the most important feedstocks for fructooligosaccharides (FOS) production. Direct enzymatic production of functional syrup from fresh JAT can benefit comprehensive processing of Jerusalem artichoke. In this study, the e...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-01-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050181 |
| _version_ | 1797957170716737536 |
|---|---|
| author | Caiyue WANG Kangming TIAN |
| author_facet | Caiyue WANG Kangming TIAN |
| author_sort | Caiyue WANG |
| collection | DOAJ |
| description | Jerusalem artichoke tuber (JAT) is rich in inulin and it is considered as one of the most important feedstocks for fructooligosaccharides (FOS) production. Direct enzymatic production of functional syrup from fresh JAT can benefit comprehensive processing of Jerusalem artichoke. In this study, the effects of various enzymes (endogenous hydrolases, endoinulinase, endoglucanase, xylanase, polygalacturonase and tannase) on fructooligosaccharide production from JAT were systematically studied and enzymatic process were further optimized. The optimal process was: first loading tannase (0.08 U/g) at 50 °C and pH 5 for 4 h, and then loading endoglucanase (0.1 U/g), xylanase (0.08 U/g), polygalacturonase (0.07 U/g) and endoinulinase (12 U/g) for 8 h. The FOS syrup was obtained by concentrated reaction solution for tow folds. The FOS and total polyphenols in FOS syrup reached 53.72 g/L and 3.11 g/L. The DPPH free radical scavenging rate, hydroxyl radical scavenging rate and total antioxidant capacity of FOS syrup were 82.23%, 30.47% and 2.78 μmol/mL, respectively. Replacing glucose in MRS medium with prepared fructooligosaccharide syrup as sole carbon source, the growth rates of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus paracasei increased by 33.33%, 60.47% and 148.15%, respectively, compared with the unenzymatically hydrolyzed Jerusalem artichoke puree as the sole carbon source of MRS medium. The main results of this study provide a theoretical basis for the enzymatic production of prebiotic food from Jerusalem artichoke. |
| first_indexed | 2024-04-11T00:00:09Z |
| format | Article |
| id | doaj.art-88f3b0218723447488db6d75f03022e2 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T00:00:09Z |
| publishDate | 2023-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-88f3b0218723447488db6d75f03022e22023-01-10T06:06:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-01-0144227027710.13386/j.issn1002-0306.20220501812022050181-2Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function EvaluationCaiyue WANG0Kangming TIAN1College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaJerusalem artichoke tuber (JAT) is rich in inulin and it is considered as one of the most important feedstocks for fructooligosaccharides (FOS) production. Direct enzymatic production of functional syrup from fresh JAT can benefit comprehensive processing of Jerusalem artichoke. In this study, the effects of various enzymes (endogenous hydrolases, endoinulinase, endoglucanase, xylanase, polygalacturonase and tannase) on fructooligosaccharide production from JAT were systematically studied and enzymatic process were further optimized. The optimal process was: first loading tannase (0.08 U/g) at 50 °C and pH 5 for 4 h, and then loading endoglucanase (0.1 U/g), xylanase (0.08 U/g), polygalacturonase (0.07 U/g) and endoinulinase (12 U/g) for 8 h. The FOS syrup was obtained by concentrated reaction solution for tow folds. The FOS and total polyphenols in FOS syrup reached 53.72 g/L and 3.11 g/L. The DPPH free radical scavenging rate, hydroxyl radical scavenging rate and total antioxidant capacity of FOS syrup were 82.23%, 30.47% and 2.78 μmol/mL, respectively. Replacing glucose in MRS medium with prepared fructooligosaccharide syrup as sole carbon source, the growth rates of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus paracasei increased by 33.33%, 60.47% and 148.15%, respectively, compared with the unenzymatically hydrolyzed Jerusalem artichoke puree as the sole carbon source of MRS medium. The main results of this study provide a theoretical basis for the enzymatic production of prebiotic food from Jerusalem artichoke.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050181jerusalem artichokeenzymatic methodenzymatic hydrolysis processfructooligosaccharidesantioxidantprebiotic function |
| spellingShingle | Caiyue WANG Kangming TIAN Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation Shipin gongye ke-ji jerusalem artichoke enzymatic method enzymatic hydrolysis process fructooligosaccharides antioxidant prebiotic function |
| title | Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation |
| title_full | Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation |
| title_fullStr | Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation |
| title_full_unstemmed | Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation |
| title_short | Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation |
| title_sort | optimization of enzymatic hydrolysis of jerusalem artichoke to produce fructooligosaccharide syrup and its function evaluation |
| topic | jerusalem artichoke enzymatic method enzymatic hydrolysis process fructooligosaccharides antioxidant prebiotic function |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050181 |
| work_keys_str_mv | AT caiyuewang optimizationofenzymatichydrolysisofjerusalemartichoketoproducefructooligosaccharidesyrupanditsfunctionevaluation AT kangmingtian optimizationofenzymatichydrolysisofjerusalemartichoketoproducefructooligosaccharidesyrupanditsfunctionevaluation |