Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo
“Tianma” (Gastrodia) and “gouteng” (Uncaria) are both widely used to treat cerebral ischemia. At the same time, “ezhu” (Curcuma longa) or turmeric, is derived from the dried roots of C. longa. It is a polyphenol known for its anti-inflammatory effects and its promotion of blood vessel endothelial fu...
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IMR Press
2020-09-01
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| 丛编: | Journal of Integrative Neuroscience |
| 主题: | |
| 在线阅读: | https://jin.imrpress.com/fileup/1757-448X/PDF/1601431078751-1817217644.pdf |
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| author | Zhi-Han Wang, Bing-Hong Chen, Ying-Ying Lin, Jin Xing, Zi-Long Wei, Li Ren |
| author_facet | Zhi-Han Wang, Bing-Hong Chen, Ying-Ying Lin, Jin Xing, Zi-Long Wei, Li Ren |
| author_sort | Zhi-Han Wang, Bing-Hong Chen, Ying-Ying Lin, Jin Xing, Zi-Long Wei, Li Ren |
| collection | DOAJ |
| description | “Tianma” (Gastrodia) and “gouteng” (Uncaria) are both widely used to treat cerebral ischemia. At the same time, “ezhu” (Curcuma longa) or turmeric, is derived from the dried roots of C. longa. It is a polyphenol known for its anti-inflammatory effects and its promotion of blood vessel endothelial function. This study explored the neuroprotective effects of a water extract of “tianma”, “gouteng”, and “ezhu” against ischemic injury. Flow cytometry analysis showed that Gastrodia, Uncaria, and Curcuma reduced the proportion of apoptotic cells in CoCl2 induced B35 (P = 0.0027) and SH-SY5Y (P = 0.0006) cell sample relative to the respective control group. Western blot indicated that Gastrodia, Uncaria, and Curcuma upregulated the expression of Bcl-2 and inversely downregulated Bax and Caspase-3 (P< 0.001). The infarct volume observed in the Gastrodia, Uncaria, and Curcuma group was also decreased compared with the control group (P < 0.05). Immunofluorescence detection revealed a lower expression of Caspase-7 in the Gastrodia, Uncaria, and Curcuma group than in the control group, while expression was negligible in the sham group. Gastrodia, Uncaria, and Curcuma confer neuroprotective effects in CoCl2 induced B35/SH-SY5Y cells and a rat model of ischemia by way of its anti-apoptotic effects. |
| first_indexed | 2024-12-10T12:26:53Z |
| format | Article |
| id | doaj.art-88f3da0647ce4ed0a07ea471cb3c2a60 |
| institution | Directory Open Access Journal |
| issn | 1757-448X |
| language | English |
| last_indexed | 2024-12-10T12:26:53Z |
| publishDate | 2020-09-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-88f3da0647ce4ed0a07ea471cb3c2a602022-12-22T01:48:57ZengIMR PressJournal of Integrative Neuroscience1757-448X2020-09-0119351351910.31083/j.jin.2020.03.002Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivoZhi-Han Wang, Bing-Hong Chen, Ying-Ying Lin, Jin Xing, Zi-Long Wei, Li Ren01Department of Neurosurgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, P. R. China;2Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China“Tianma” (Gastrodia) and “gouteng” (Uncaria) are both widely used to treat cerebral ischemia. At the same time, “ezhu” (Curcuma longa) or turmeric, is derived from the dried roots of C. longa. It is a polyphenol known for its anti-inflammatory effects and its promotion of blood vessel endothelial function. This study explored the neuroprotective effects of a water extract of “tianma”, “gouteng”, and “ezhu” against ischemic injury. Flow cytometry analysis showed that Gastrodia, Uncaria, and Curcuma reduced the proportion of apoptotic cells in CoCl2 induced B35 (P = 0.0027) and SH-SY5Y (P = 0.0006) cell sample relative to the respective control group. Western blot indicated that Gastrodia, Uncaria, and Curcuma upregulated the expression of Bcl-2 and inversely downregulated Bax and Caspase-3 (P< 0.001). The infarct volume observed in the Gastrodia, Uncaria, and Curcuma group was also decreased compared with the control group (P < 0.05). Immunofluorescence detection revealed a lower expression of Caspase-7 in the Gastrodia, Uncaria, and Curcuma group than in the control group, while expression was negligible in the sham group. Gastrodia, Uncaria, and Curcuma confer neuroprotective effects in CoCl2 induced B35/SH-SY5Y cells and a rat model of ischemia by way of its anti-apoptotic effects.https://jin.imrpress.com/fileup/1757-448X/PDF/1601431078751-1817217644.pdf|alternative medicine|chinese herbal medicine|gastrodia|uncaria|curcuma|cerebral ischemia|apoptosis |
| spellingShingle | Zhi-Han Wang, Bing-Hong Chen, Ying-Ying Lin, Jin Xing, Zi-Long Wei, Li Ren Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo Journal of Integrative Neuroscience |alternative medicine|chinese herbal medicine|gastrodia|uncaria|curcuma|cerebral ischemia|apoptosis |
| title | Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| title_full | Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| title_fullStr | Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| title_full_unstemmed | Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| title_short | Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| title_sort | herbal decoction of gastrodia uncaria and curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo |
| topic | |alternative medicine|chinese herbal medicine|gastrodia|uncaria|curcuma|cerebral ischemia|apoptosis |
| url | https://jin.imrpress.com/fileup/1757-448X/PDF/1601431078751-1817217644.pdf |
| work_keys_str_mv | AT zhihanwangbinghongchenyingyinglinjinxingzilongweiliren herbaldecoctionofgastrodiauncariaandcurcumaconfersneuroprotectionagainstcerebralischemiainvitroandinvivo |