Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model
Abstract Background An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may al...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-020-01953-7 |
| _version_ | 1811187083478302720 |
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| author | Shintaro Shoji Kentaro Uchida Wataru Satio Hiroyuki Sekiguchi Gen Inoue Masayuki Miyagi Ken Takata Yuji Yokozeki Masashi Takaso |
| author_facet | Shintaro Shoji Kentaro Uchida Wataru Satio Hiroyuki Sekiguchi Gen Inoue Masayuki Miyagi Ken Takata Yuji Yokozeki Masashi Takaso |
| author_sort | Shintaro Shoji |
| collection | DOAJ |
| description | Abstract Background An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for bone morphogenetic protein (BMP)-2 has yet to be examined. Here, we examined the effect of an IFH made of hyaluronic acid (IFH-HA) containing BMP-2 in promoting osteogenesis in a mouse refractory fracture model. Methods Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-HA/PBS or IFH-HA containing 2 μg BMP-2 (IFH-HA/BMP-2) into the fracture site (n = 16, each treatment). Results Fracture sites injected with IFH-HA/BMP-2 showed significantly greater bone volume, bone mineral content, and bone union compared with sites receiving no treatment or treated with IFH-HA/PBS alone (each n = 10). Gene expression levels of osteogenic markers, Alpl, Bglap, and Osx, were significantly raised in the IFH-HA/BMP-2 group compared to the IFH-HA/PBS and control groups (each n = 6). Conclusion IFH-HA/BMP-2 may contribute to the treatment of refractory fractures. |
| first_indexed | 2024-04-11T13:57:11Z |
| format | Article |
| id | doaj.art-88f4a7faab0549f9a41715ad45903c55 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T13:57:11Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-88f4a7faab0549f9a41715ad45903c552022-12-22T04:20:18ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-09-011511810.1186/s13018-020-01953-7Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture modelShintaro Shoji0Kentaro Uchida1Wataru Satio2Hiroyuki Sekiguchi3Gen Inoue4Masayuki Miyagi5Ken Takata6Yuji Yokozeki7Masashi Takaso8Department of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineShonan University of Medical Sciences Research InstituteDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineAbstract Background An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for bone morphogenetic protein (BMP)-2 has yet to be examined. Here, we examined the effect of an IFH made of hyaluronic acid (IFH-HA) containing BMP-2 in promoting osteogenesis in a mouse refractory fracture model. Methods Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-HA/PBS or IFH-HA containing 2 μg BMP-2 (IFH-HA/BMP-2) into the fracture site (n = 16, each treatment). Results Fracture sites injected with IFH-HA/BMP-2 showed significantly greater bone volume, bone mineral content, and bone union compared with sites receiving no treatment or treated with IFH-HA/PBS alone (each n = 10). Gene expression levels of osteogenic markers, Alpl, Bglap, and Osx, were significantly raised in the IFH-HA/BMP-2 group compared to the IFH-HA/PBS and control groups (each n = 6). Conclusion IFH-HA/BMP-2 may contribute to the treatment of refractory fractures.http://link.springer.com/article/10.1186/s13018-020-01953-7In situ-formed hydrogelsHyaluronic acidBone morphogenetic proteinRefractory fracture |
| spellingShingle | Shintaro Shoji Kentaro Uchida Wataru Satio Hiroyuki Sekiguchi Gen Inoue Masayuki Miyagi Ken Takata Yuji Yokozeki Masashi Takaso Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model Journal of Orthopaedic Surgery and Research In situ-formed hydrogels Hyaluronic acid Bone morphogenetic protein Refractory fracture |
| title | Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model |
| title_full | Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model |
| title_fullStr | Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model |
| title_full_unstemmed | Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model |
| title_short | Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model |
| title_sort | acceleration of bone union by in situ formed hydrogel containing bone morphogenetic protein 2 in a mouse refractory fracture model |
| topic | In situ-formed hydrogels Hyaluronic acid Bone morphogenetic protein Refractory fracture |
| url | http://link.springer.com/article/10.1186/s13018-020-01953-7 |
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