Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
Modeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-02-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-017-0011-2 |
| _version_ | 1797641217163395072 |
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| author | Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson |
| author_facet | Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson |
| author_sort | Jennifer Barrila |
| collection | DOAJ |
| description | Modeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D) intestinal co-culture model containing multiple differentiated epithelial cell types and phagocytic macrophages with antibacterial function to study infection by multiple pathovars of Salmonella. This study is the first to show that these pathovars (known to possess different host adaptations, antibiotic resistance profiles and disease phenotypes), display markedly different colonization and intracellular co-localization patterns using this physiologically relevant new 3-D intestinal co-culture model. This advanced model, that integrates a key immune cell type important for Salmonella infection, offers a powerful new tool in understanding enteric pathogenesis and may lead to unexpected pathogenesis mechanisms and therapeutic targets that have been previously unobserved or unappreciated using other intestinal cell culture models. |
| first_indexed | 2024-03-11T13:42:25Z |
| format | Article |
| id | doaj.art-88fd895a1fbb40f4a1f1b233ee036835 |
| institution | Directory Open Access Journal |
| issn | 2373-8065 |
| language | English |
| last_indexed | 2024-03-11T13:42:25Z |
| publishDate | 2017-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj.art-88fd895a1fbb40f4a1f1b233ee0368352023-11-02T11:35:08ZengNature Portfolionpj Microgravity2373-80652017-02-013111210.1038/s41526-017-0011-2Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patternsJennifer Barrila0Jiseon Yang1Aurélie Crabbé2Shameema F. Sarker3Yulong Liu4C. Mark Ott5Mayra A. Nelman-Gonzalez6Simon J. Clemett7Seth D. Nydam8Rebecca J. Forsyth9Richard R. Davis10Brian E. Crucian11Heather Quiriarte12Kenneth L. Roland13Karen Brenneman14Clarence Sams15Christine Loscher16Cheryl A. Nickerson17Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityBiomedical Research and Environmental Sciences Division, NASA Johnson Space CenterWyle Science, Technology and Engineering GroupERC, Inc/Jacobs JETSCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityBiomedical Research and Environmental Sciences Division, NASA Johnson Space CenterJES TechCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityBiomedical Research and Environmental Sciences Division, NASA Johnson Space CenterImmunomodulation Research Group, School of Biotechnology, Dublin City UniversityCenter for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State UniversityModeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D) intestinal co-culture model containing multiple differentiated epithelial cell types and phagocytic macrophages with antibacterial function to study infection by multiple pathovars of Salmonella. This study is the first to show that these pathovars (known to possess different host adaptations, antibiotic resistance profiles and disease phenotypes), display markedly different colonization and intracellular co-localization patterns using this physiologically relevant new 3-D intestinal co-culture model. This advanced model, that integrates a key immune cell type important for Salmonella infection, offers a powerful new tool in understanding enteric pathogenesis and may lead to unexpected pathogenesis mechanisms and therapeutic targets that have been previously unobserved or unappreciated using other intestinal cell culture models.https://doi.org/10.1038/s41526-017-0011-2 |
| spellingShingle | Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns npj Microgravity |
| title | Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns |
| title_full | Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns |
| title_fullStr | Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns |
| title_full_unstemmed | Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns |
| title_short | Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns |
| title_sort | three dimensional organotypic co culture model of intestinal epithelial cells and macrophages to study salmonella enterica colonization patterns |
| url | https://doi.org/10.1038/s41526-017-0011-2 |
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