Comparison of Laboratory Methods for Detection of Pneumocystis carinii
Detection of P. carinii is increased by the use of polymerase chain reaction (PCR), particularly on sputum samples. In this study, sputum samples obtained from 30 immunosuppressed patients with respiratory symptoms (12 HIV infected) were tested by standard cytochemical staining (Giemsa and methenami...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
1998-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1998-3-1-020-025.pdf |
| _version_ | 1797921326685487104 |
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| author | Serdar TUNCER Sibel ERGÜVEN Sesin KOCAGÖZ Serhat ÜNAL |
| author_facet | Serdar TUNCER Sibel ERGÜVEN Sesin KOCAGÖZ Serhat ÜNAL |
| author_sort | Serdar TUNCER |
| collection | DOAJ |
| description | Detection of P. carinii is increased by the use of polymerase chain reaction (PCR), particularly on sputum samples. In this study, sputum samples obtained from 30 immunosuppressed patients with respiratory symptoms (12 HIV infected) were tested by standard cytochemical staining (Giemsa and methenamine silver), immunofluorescense (IF) staining and PCR for detection of P. carinii and the results were compared. P. carinii were detected in 4, 8, and 13 sputum samples by cytological staining, IF test and PCR, respectively. Specific amplification bands were obtained in all sputum samples which were positive by both other tests. All tests gave negative results in sputum samples obtained from 5 HIV infected asymptomatic patients. Our observations suggest that PCR results were found to be well correlated with P. carinii pneumonia (PCP) especially in non-HIV infected patients. However, PCR positivity obtained on HIV infected patients could be misleading in the diagnosis of PCP without careful clinical evaluation. Positive results obtained by Giemsa staining or IF test confirm diagnosis of PCP with high degree correlation. As a result, we suggest testing sputum samples by both PCR and IF techniques for detection of P. carinii. |
| first_indexed | 2024-04-10T14:14:21Z |
| format | Article |
| id | doaj.art-88fde454da114d82843e9305f7c38a14 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T14:14:21Z |
| publishDate | 1998-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-88fde454da114d82843e9305f7c38a142023-02-15T16:09:36ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X1998-03-01312025Comparison of Laboratory Methods for Detection of Pneumocystis cariniiSerdar TUNCER0Sibel ERGÜVEN1Sesin KOCAGÖZ2Serhat ÜNAL3Hacettepe Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Ünitesi,Hacettepe Üniversitesi Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, ANKARAHacettepe Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Ünitesi,Hacettepe Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Ünitesi,Detection of P. carinii is increased by the use of polymerase chain reaction (PCR), particularly on sputum samples. In this study, sputum samples obtained from 30 immunosuppressed patients with respiratory symptoms (12 HIV infected) were tested by standard cytochemical staining (Giemsa and methenamine silver), immunofluorescense (IF) staining and PCR for detection of P. carinii and the results were compared. P. carinii were detected in 4, 8, and 13 sputum samples by cytological staining, IF test and PCR, respectively. Specific amplification bands were obtained in all sputum samples which were positive by both other tests. All tests gave negative results in sputum samples obtained from 5 HIV infected asymptomatic patients. Our observations suggest that PCR results were found to be well correlated with P. carinii pneumonia (PCP) especially in non-HIV infected patients. However, PCR positivity obtained on HIV infected patients could be misleading in the diagnosis of PCP without careful clinical evaluation. Positive results obtained by Giemsa staining or IF test confirm diagnosis of PCP with high degree correlation. As a result, we suggest testing sputum samples by both PCR and IF techniques for detection of P. carinii.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1998-3-1-020-025.pdfPneumocystis cariniDiagnosisImmunofluorescensePCR |
| spellingShingle | Serdar TUNCER Sibel ERGÜVEN Sesin KOCAGÖZ Serhat ÜNAL Comparison of Laboratory Methods for Detection of Pneumocystis carinii Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Pneumocystis carini Diagnosis Immunofluorescense PCR |
| title | Comparison of Laboratory Methods for Detection of Pneumocystis carinii |
| title_full | Comparison of Laboratory Methods for Detection of Pneumocystis carinii |
| title_fullStr | Comparison of Laboratory Methods for Detection of Pneumocystis carinii |
| title_full_unstemmed | Comparison of Laboratory Methods for Detection of Pneumocystis carinii |
| title_short | Comparison of Laboratory Methods for Detection of Pneumocystis carinii |
| title_sort | comparison of laboratory methods for detection of pneumocystis carinii |
| topic | Pneumocystis carini Diagnosis Immunofluorescense PCR |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1998-3-1-020-025.pdf |
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