Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
Matrix production by nucleus pulposus (NP) cells, the cells residing in the center of the intervertebral disc, can be stimulated by growth factors. Bone morphogenetic proteins (BMPs) hold great promise. Although BMP2 and BMP7 have been used most frequently, other BMPs have also shown potential for N...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2720 |
| _version_ | 1797570656755253248 |
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| author | Anita Krouwels Juvita D. Iljas Angela H. M. Kragten Wouter J. A. Dhert F. Cumhur Öner Marianna A. Tryfonidou Laura B. Creemers |
| author_facet | Anita Krouwels Juvita D. Iljas Angela H. M. Kragten Wouter J. A. Dhert F. Cumhur Öner Marianna A. Tryfonidou Laura B. Creemers |
| author_sort | Anita Krouwels |
| collection | DOAJ |
| description | Matrix production by nucleus pulposus (NP) cells, the cells residing in the center of the intervertebral disc, can be stimulated by growth factors. Bone morphogenetic proteins (BMPs) hold great promise. Although BMP2 and BMP7 have been used most frequently, other BMPs have also shown potential for NP regeneration. Heterodimers may be more potent than single homodimers, but it is not known whether combinations of homodimers would perform equally well. In this study, we compared BMP2, BMP4, BMP6, and BMP7, their combinations and heterodimers, for regeneration by human NP cells. The BMPs investigated induced variable matrix deposition by NP cells. BMP4 was the most potent, both in the final neotissue glysosaminoglycan content and incorporation efficiency. Heterodimers BMP2/6H and BMP2/7H were more potent than their respective homodimer combinations, but not the BMP4/7H heterodimer. The current results indicate that BMP4 might have a high potential for regeneration of the intervertebral disc. Moreover, the added value of BMP heterodimers over their respective homodimer BMP combinations depends on the BMP combination applied. |
| first_indexed | 2024-03-10T20:28:16Z |
| format | Article |
| id | doaj.art-890050feee0c4e4aa1e09fbceff95fe3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:28:16Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-890050feee0c4e4aa1e09fbceff95fe32023-11-19T21:37:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218272010.3390/ijms21082720Bone Morphogenetic Proteins for Nucleus Pulposus RegenerationAnita Krouwels0Juvita D. Iljas1Angela H. M. Kragten2Wouter J. A. Dhert3F. Cumhur Öner4Marianna A. Tryfonidou5Laura B. Creemers6Department of Orthopedics, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Orthopedics, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Orthopedics, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsFaculty of Veterinary Medicine, Utrecht University, 3584 CS Utrecht, The NetherlandsDepartment of Orthopedics, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CS Utrecht, The NetherlandsDepartment of Orthopedics, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsMatrix production by nucleus pulposus (NP) cells, the cells residing in the center of the intervertebral disc, can be stimulated by growth factors. Bone morphogenetic proteins (BMPs) hold great promise. Although BMP2 and BMP7 have been used most frequently, other BMPs have also shown potential for NP regeneration. Heterodimers may be more potent than single homodimers, but it is not known whether combinations of homodimers would perform equally well. In this study, we compared BMP2, BMP4, BMP6, and BMP7, their combinations and heterodimers, for regeneration by human NP cells. The BMPs investigated induced variable matrix deposition by NP cells. BMP4 was the most potent, both in the final neotissue glysosaminoglycan content and incorporation efficiency. Heterodimers BMP2/6H and BMP2/7H were more potent than their respective homodimer combinations, but not the BMP4/7H heterodimer. The current results indicate that BMP4 might have a high potential for regeneration of the intervertebral disc. Moreover, the added value of BMP heterodimers over their respective homodimer BMP combinations depends on the BMP combination applied.https://www.mdpi.com/1422-0067/21/8/2720nucleus pulposusregenerationbone morphogenetic proteinheterodimerproteoglycancollagen |
| spellingShingle | Anita Krouwels Juvita D. Iljas Angela H. M. Kragten Wouter J. A. Dhert F. Cumhur Öner Marianna A. Tryfonidou Laura B. Creemers Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration International Journal of Molecular Sciences nucleus pulposus regeneration bone morphogenetic protein heterodimer proteoglycan collagen |
| title | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration |
| title_full | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration |
| title_fullStr | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration |
| title_full_unstemmed | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration |
| title_short | Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration |
| title_sort | bone morphogenetic proteins for nucleus pulposus regeneration |
| topic | nucleus pulposus regeneration bone morphogenetic protein heterodimer proteoglycan collagen |
| url | https://www.mdpi.com/1422-0067/21/8/2720 |
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