Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes
Curcumin is known for its anti-inflammatory, neuroprotective, and antioxidant properties, but its use in biological applications is hindered by its sensitivity to light, oxygen, and temperature. Furthermore, due to its low water solubility, curcumin has a poor pharmacokinetic profile and bioavailabi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/12/2665 |
| _version_ | 1797379721882763264 |
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| author | Amina Ben Mihoub Kamil Elkhoury Janske Nel Samir Acherar Emilie Velot Catherine Malaplate Michel Linder Shahrzad Latifi Cyril Kahn Marion Huguet Frances T. Yen Elmira Arab-Tehrany |
| author_facet | Amina Ben Mihoub Kamil Elkhoury Janske Nel Samir Acherar Emilie Velot Catherine Malaplate Michel Linder Shahrzad Latifi Cyril Kahn Marion Huguet Frances T. Yen Elmira Arab-Tehrany |
| author_sort | Amina Ben Mihoub |
| collection | DOAJ |
| description | Curcumin is known for its anti-inflammatory, neuroprotective, and antioxidant properties, but its use in biological applications is hindered by its sensitivity to light, oxygen, and temperature. Furthermore, due to its low water solubility, curcumin has a poor pharmacokinetic profile and bioavailability. In this study, we evaluated the potential application of curcumin as a neuroprotective agent encapsulated in RGD peptide-PEGylated nanoliposomes developed from salmon-derived lecithin. Salmon lecithin, rich in polyunsaturated fatty acids, was used to formulate empty or curcumin-loaded nanoliposomes. Transmission electron microscopy, dynamic light scattering, and nanoparticle tracking analysis characterizations indicated that the marine-derived peptide-PEGylated nanoliposomes were spherical in shape, nanometric in size, and with an overall negative charge. Cytotoxicity tests of curcumin-loaded nanoliposomes revealed an improved tolerance of neurons to curcumin as compared to free curcumin. Wild-type SH-SY5Y were treated for 24 h with curcumin-loaded nanoliposomes, followed by 24 h incubation with conditioned media of SH-SY5Y expressing the Swedish mutation of APP containing a high ratio of Aβ40/42 peptides. Our results revealed significantly lower Aβ-induced cell toxicity in cells pre-treated with RGD peptide-PEGylated curcumin-loaded nanoliposomes, as compared to controls. Thus, our data highlight the potential use of salmon lecithin-derived RGD peptide PEGylated nanoliposomes for the efficient drug delivery of curcumin as a neuroprotective agent. |
| first_indexed | 2024-03-08T20:27:05Z |
| format | Article |
| id | doaj.art-8901126482904605ae973d5cc0724168 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T20:27:05Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8901126482904605ae973d5cc07241682023-12-22T14:31:54ZengMDPI AGPharmaceutics1999-49232023-11-011512266510.3390/pharmaceutics15122665Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated NanoliposomesAmina Ben Mihoub0Kamil Elkhoury1Janske Nel2Samir Acherar3Emilie Velot4Catherine Malaplate5Michel Linder6Shahrzad Latifi7Cyril Kahn8Marion Huguet9Frances T. Yen10Elmira Arab-Tehrany11LIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceLIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceLIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceLCPM, CNRS, University of Lorraine, F-54000 Nancy, FranceIMoPA, CNRS, University of Lorraine, F-54000 Nancy, FranceUR AFPA Laboratory, Qualivie Team, University of Lorraine, F-54000 Nancy, FranceLIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceDepartment of Neuroscience, Rockefeller Neuroscience Institute, West Virginia University, Morgantown, WV 26506, USALIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceUR AFPA Laboratory, Qualivie Team, University of Lorraine, F-54000 Nancy, FranceUR AFPA Laboratory, Qualivie Team, University of Lorraine, F-54000 Nancy, FranceLIBio Laboratory, University of Lorraine, F-54000 Nancy, FranceCurcumin is known for its anti-inflammatory, neuroprotective, and antioxidant properties, but its use in biological applications is hindered by its sensitivity to light, oxygen, and temperature. Furthermore, due to its low water solubility, curcumin has a poor pharmacokinetic profile and bioavailability. In this study, we evaluated the potential application of curcumin as a neuroprotective agent encapsulated in RGD peptide-PEGylated nanoliposomes developed from salmon-derived lecithin. Salmon lecithin, rich in polyunsaturated fatty acids, was used to formulate empty or curcumin-loaded nanoliposomes. Transmission electron microscopy, dynamic light scattering, and nanoparticle tracking analysis characterizations indicated that the marine-derived peptide-PEGylated nanoliposomes were spherical in shape, nanometric in size, and with an overall negative charge. Cytotoxicity tests of curcumin-loaded nanoliposomes revealed an improved tolerance of neurons to curcumin as compared to free curcumin. Wild-type SH-SY5Y were treated for 24 h with curcumin-loaded nanoliposomes, followed by 24 h incubation with conditioned media of SH-SY5Y expressing the Swedish mutation of APP containing a high ratio of Aβ40/42 peptides. Our results revealed significantly lower Aβ-induced cell toxicity in cells pre-treated with RGD peptide-PEGylated curcumin-loaded nanoliposomes, as compared to controls. Thus, our data highlight the potential use of salmon lecithin-derived RGD peptide PEGylated nanoliposomes for the efficient drug delivery of curcumin as a neuroprotective agent.https://www.mdpi.com/1999-4923/15/12/2665nanoliposomescurcuminPEGtargeting peptidescortical neuronsneuroprotection |
| spellingShingle | Amina Ben Mihoub Kamil Elkhoury Janske Nel Samir Acherar Emilie Velot Catherine Malaplate Michel Linder Shahrzad Latifi Cyril Kahn Marion Huguet Frances T. Yen Elmira Arab-Tehrany Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes Pharmaceutics nanoliposomes curcumin PEG targeting peptides cortical neurons neuroprotection |
| title | Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes |
| title_full | Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes |
| title_fullStr | Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes |
| title_full_unstemmed | Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes |
| title_short | Neuroprotective Effect of Curcumin-Loaded RGD Peptide-PEGylated Nanoliposomes |
| title_sort | neuroprotective effect of curcumin loaded rgd peptide pegylated nanoliposomes |
| topic | nanoliposomes curcumin PEG targeting peptides cortical neurons neuroprotection |
| url | https://www.mdpi.com/1999-4923/15/12/2665 |
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