Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice
The delayed and incomplete healing of diabetic wounds remains a major concern of global healthcare. The complex biological processes within the diabetic wound, such as chronic inflammation, impaired blood vessel growth and immature collagen remodeling, dramatically cause the failure of current treat...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.898231/full |
| _version_ | 1818551457336524800 |
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| author | Limei Wang Kedi Xia Lu Han Min Zhang Jihuan Fan Liu Song Anqi Liao Wenyu Wang Jianfeng Guo |
| author_facet | Limei Wang Kedi Xia Lu Han Min Zhang Jihuan Fan Liu Song Anqi Liao Wenyu Wang Jianfeng Guo |
| author_sort | Limei Wang |
| collection | DOAJ |
| description | The delayed and incomplete healing of diabetic wounds remains a major concern of global healthcare. The complex biological processes within the diabetic wound, such as chronic inflammation, impaired blood vessel growth and immature collagen remodeling, dramatically cause the failure of current treatments. Thus, emerging therapeutic strategies are highly desirable. Ginkgolide B (GB, a natural product extracted from the leaves of Ginkgo biloba L.) has been applied in the treatment of cerebrovascular and cardiovascular disorders, which is mainly due to the anti-oxidative, anti-inflammatory and proliferative effects. In this study, the role of GB in facilitating the anti-inflammatory and pro-healing effects on diabetic wounds was for the first time confirmed using in vitro, ex vivo and in vivo experimental methods. As a consequence, GB was able to significantly achieve the activities of anti-inflammation, re-epithelialization, and pro-angiogenesis. Previously, a hydrogel has been developed using the high molecular weight hyaluronan (hyaluronic acid, HA) in our laboratory. In this study, this hydrogel was utilized in vivo for local administration of GB to the full-thickness wounds of diabetic mice. The resultant hydrogel formulation (HA-GB) resulted in the reduction of inflammation, the enhancement of re-epithelialization and angiogenesis, and the modulation of collagens from type III to type I, significantly promoting the healing outcome as compared with a commercially available wound dressing product (INTRASITE Gel). This study confirms a great therapeutic promise of HA-GB for the chronic wounds of diabetic patients. |
| first_indexed | 2024-12-12T09:00:09Z |
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| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T09:00:09Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-89050d440044438190d00193ee8a19332022-12-22T00:29:51ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-05-011010.3389/fbioe.2022.898231898231Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic MiceLimei Wang0Kedi Xia1Lu Han2Min Zhang3Jihuan Fan4Liu Song5Anqi Liao6Wenyu Wang7Jianfeng Guo8Department of Pharmacy, Jilin Province FAW General Hospital, Changchun, ChinaDepartment of Pharmacy, Jilin Province FAW General Hospital, Changchun, ChinaDepartment of Medical Administration, Jilin Province FAW General Hospital, Changchun, ChinaDepartment of Ophthalmology and Otorhinolaryngology, Jilin Province FAW General Hospital, Changchun, ChinaDepartment of Education and Science Services, Jilin Province FAW General Hospital, Changchun, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, ChinaDepartment of Thoracic Surgery, Jilin Province FAW General Hospital, Changchun, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, ChinaThe delayed and incomplete healing of diabetic wounds remains a major concern of global healthcare. The complex biological processes within the diabetic wound, such as chronic inflammation, impaired blood vessel growth and immature collagen remodeling, dramatically cause the failure of current treatments. Thus, emerging therapeutic strategies are highly desirable. Ginkgolide B (GB, a natural product extracted from the leaves of Ginkgo biloba L.) has been applied in the treatment of cerebrovascular and cardiovascular disorders, which is mainly due to the anti-oxidative, anti-inflammatory and proliferative effects. In this study, the role of GB in facilitating the anti-inflammatory and pro-healing effects on diabetic wounds was for the first time confirmed using in vitro, ex vivo and in vivo experimental methods. As a consequence, GB was able to significantly achieve the activities of anti-inflammation, re-epithelialization, and pro-angiogenesis. Previously, a hydrogel has been developed using the high molecular weight hyaluronan (hyaluronic acid, HA) in our laboratory. In this study, this hydrogel was utilized in vivo for local administration of GB to the full-thickness wounds of diabetic mice. The resultant hydrogel formulation (HA-GB) resulted in the reduction of inflammation, the enhancement of re-epithelialization and angiogenesis, and the modulation of collagens from type III to type I, significantly promoting the healing outcome as compared with a commercially available wound dressing product (INTRASITE Gel). This study confirms a great therapeutic promise of HA-GB for the chronic wounds of diabetic patients.https://www.frontiersin.org/articles/10.3389/fbioe.2022.898231/fullhyaluronic aciddrug deliverybiomaterialsnatural productsregenerative medicine |
| spellingShingle | Limei Wang Kedi Xia Lu Han Min Zhang Jihuan Fan Liu Song Anqi Liao Wenyu Wang Jianfeng Guo Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice Frontiers in Bioengineering and Biotechnology hyaluronic acid drug delivery biomaterials natural products regenerative medicine |
| title | Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice |
| title_full | Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice |
| title_fullStr | Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice |
| title_full_unstemmed | Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice |
| title_short | Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice |
| title_sort | local administration of ginkgolide b using a hyaluronan based hydrogel improves wound healing in diabetic mice |
| topic | hyaluronic acid drug delivery biomaterials natural products regenerative medicine |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.898231/full |
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